Effects of Argonaute on Gene Expression in Thermus thermophilus. [PDF]
PLoS ONE, 2015 Eukaryotic Argonaute proteins mediate RNA-guided RNA interference, allowing both regulation of host gene expression and defense against invading mobile genetic elements.
Daan C Swarts +4 more
doaj +6 more sources
Rapid method for plasmid DNA recombination (Murakami-system) [PDF]
MethodsX, 2023 DNA recombination is a useful technology for cloning and subsequent functional analysis, while standard techniques for plasmid DNA recombination have remained unchanged.
Agnieszka M. Murakami +5 more
doaj +2 more sources
Induction of translation-suppressive G3BP1+ stress granules and interferon-signaling cGAS condensates by transfected plasmid DNA [PDF]
hLifePlasmid DNA transfection is one of the fundamental tools of biomedical research. Here, we found that plasmid DNA transfection mediated by liposomes activates multiple innate immune responses in several widely used cell lines.
Vladimir Majerciak, Zhi-Ming Zheng
doaj +2 more sources
A new device-mediated miniprep method
AMB Express, 2022 Keypoints New devise-mediated plasmid miniprep method (DM) performs all mixing steps without operator intervention. The DM method produces plasmid DNAs free of the dCCC form and significantly reduces the contamination with genomic DNA in the low-copy ...
Baryshev Mikhail +2 more
doaj +1 more source
Separation Properties of Plasmid DNA Using a Two-Stage Particle Adsorption-Microfiltration Process
Membranes, 2023 Plasmid DNA is used as a vector for gene therapy and DNA vaccination; therefore, the establishment of a mass production method is required. Membrane filtration is widely employed as a separation method suitable for the mass production of plasmid DNA ...
Nobuyuki Katagiri +4 more
doaj +1 more source
Application of gene therapy in the treatment of superficial digital flexor tendon injury in horses [PDF]
Open Veterinary Journal, 2020 Background: Tendon injuries are one of the most common causes of orthopedic disorders in horses. Such injuries involve a long course of treatment and recovery.
Alexandr Aimaletdinov +8 more
doaj +1 more source
Quantification bias caused by plasmid DNA conformation in quantitative real-time PCR assay. [PDF]
PLoS ONE, 2011 Quantitative real-time PCR (qPCR) is the gold standard for the quantification of specific nucleic acid sequences. However, a serious concern has been revealed in a recent report: supercoiled plasmid standards cause significant over-estimation in qPCR ...
Chih-Hui Lin +2 more
doaj +1 more source
The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields. [PDF]
PLoS ONE, 2016 Plasmid purification is a basic tool of molecular biologists. Although the development of plasmid isolation kits utilizing silica spin columns reduced the time and labor spent on plasmid purification, achieving large plasmid DNA yields still requires ...
Mira I Pronobis +2 more
doaj +1 more source
Сибирский научный медицинский журнал, 2023 The aim of this study was to investigate the possibility of using apolipoprotein A-I (apo A-I) as a transport form of the green fluorescent protein (GFP) gene into rat hepatocytes.Material and methods.
L. M. Polyakov +4 more
doaj +1 more source
Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]
, 1993 The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini +3 more
core +2 more sources