European Cells & Materials, 2023 Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović +9 more
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Repository-based plasmid design [PDF]
, 2020 There was an explosion in the amount of commercially available DNA in sequence repositories over the last decade. The number of such plasmids increased from 12,000 to over 300,000 among three of the largest repositories: iGEM, Addgene, and DNASU.
Densmore, Doug, Timmons, Joshua J.
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Transcription of plasmid DNA: Influence of plasmid DNA/polyethylenimine complex formation [PDF]
Journal of Controlled Release, 2005 Polyethylenimine (PEI) is one of the most potent non-viral vectors. We have developed a lactosylated PEI (Lac-PEI) to enhance cell-specific transfection and have shown that Lac-PEI is more efficient than unsubstituted PEI for gene transfer into immortalized cystic fibrosis airway epithelial SigmaCFTE29o-cells.
Honoré, I. +5 more
openaire +2 more sources
Sending Plasmid DNA by Mail [PDF]
BioTechniques, 2001 Michael W. Mortimer, Michael L. Kahn
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The integrity of chemically treated plasmid DNA as a chemical-based choice for prion clearance
Regenerative Therapy, 2020 In regenerative medical products for clinical applications, a major concern is the risk of ruminant-derived materials developing transmissible spongiform encephalopathy (TSE) in the manufacturing process. Because of the risk of TSE causing prion disease,
Nozomi Takada +5 more
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RpoS regulates a novel type of plasmid DNA transfer in Escherichia coli. [PDF]
PLoS ONE, 2012 Spontaneous plasmid transformation of Escherichia coli is independent of the DNA uptake machinery for single-stranded DNA (ssDNA) entry. The one-hit kinetic pattern of plasmid transformation indicates that double-stranded DNA (dsDNA) enters E. coli cells
Yanmei Zhang +4 more
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Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]
, 2012 Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA +4 more
core +1 more source
Comparison of the effectiveness of chlorine, ozone, and photocatalytic disinfection in reducing the risk of antibiotic resistance pollution [PDF]
, 2011 Effectiveness of conventional chlorine and ozone disinfection on reduction of antibiotic resistance was compared with less commonly applied heterogeneous photocatalytic process.
Balcioglu, Isil Akmehmet +5 more
core +1 more source
Regulatory T cells suppress natural killer cells during plasmid DNA vaccination in mice, blunting the CD8+ T cell immune response by the cytokine TGFbeta. [PDF]
PLoS ONE, 2010 CD4(+)CD25(+) regulatory T cells (Tregs) suppress adaptive T cell-mediated immune responses to self- and foreign-antigens. Tregs may also suppress early innate immune responses to vaccine antigens and might decrease vaccine efficacy. NK and NKT cells are
Kwesi Frimpong-Boateng +2 more
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