Results 251 to 260 of about 982,599 (302)
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Current Drug Targets - Immune, Endocrine & Metabolic Disorders, 2001
Genes that code for the production of protein antigens have been cloned and recombined with plasmids. Gene-plasmid constructs have been amplified in a bacterial host, purified and administered to a mammalian host. The gene is expressed in the host and the antigen that is produced induces an immune response.
G, Mor, M, Eliza
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Genes that code for the production of protein antigens have been cloned and recombined with plasmids. Gene-plasmid constructs have been amplified in a bacterial host, purified and administered to a mammalian host. The gene is expressed in the host and the antigen that is produced induces an immune response.
G, Mor, M, Eliza
openaire +4 more sources
Dephosphorylation of Plasmid DNA
Cold Spring Harbor Protocols, 2020This protocol presents a method to remove 5′-phosphate residues from protruding or blunt termini of linearized plasmids. This suppresses the recircularization of plasmid DNA during subsequent ligation procedures.
Michael R, Green, Joseph, Sambrook
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The Journal of Gene Medicine, 2004
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non-viral over viral vectors.A prerequisite for the success of plasmid-based therapies is the development of cost-effective and ...
Joachim, Stadler +2 more
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The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non-viral over viral vectors.A prerequisite for the success of plasmid-based therapies is the development of cost-effective and ...
Joachim, Stadler +2 more
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Journal of Chromatography A, 2005
Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purification step, it is also very useful as an analytical tool to monitor and control pDNA quality during processing and in final formulations.
M M, Diogo +2 more
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Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purification step, it is also very useful as an analytical tool to monitor and control pDNA quality during processing and in final formulations.
M M, Diogo +2 more
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Plasmid DNA Manufacturing Technology
Recent Patents on Biotechnology, 2007Today, plasmid DNA is becoming increasingly important as the next generation of biotechnology products (gene medicines and DNA vaccines) make their way into clinical trials, and eventually into the pharmaceutical marketplace. This review summarizes recent patents and patent applications relating to plasmid manufacturing, in the context of a ...
Aaron E, Carnes, James A, Williams
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Quick Transformation with Plasmid DNA
Cold Spring Harbor Protocols, 2022Genomic engineering of Escherichia coli and Salmonella often requires introducing plasmids into strains obtained during the intermediate stages of the process. Such strains are typically transformed only once, making the preparation of large batches of competent cells for storage purposes unnecessary.
Nara Figueroa-Bossi +2 more
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Preparing Plasmid DNA from Bacteria
Cold Spring Harbor Protocols, 2022The most common method for isolating plasmid DNA is derived from an alkaline lysis procedure. The procedure exploits the differential partitioning of plasmid and chromosomal DNA when denatured by alkali and subsequently renatured by neutralization of the medium.
Nara Figueroa-Bossi +2 more
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Annual Review of Microbiology, 1983
The study of yeast DNA plasmids has been initiated with the discovery of the 2-micron DNA in Saccharomyces cerevisiae. This multiple copy plasmid, organized into chromatin structure in vivo, probably exists in the nucleus and provides a good system to obtain information on eukaryotic DNA replication.
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The study of yeast DNA plasmids has been initiated with the discovery of the 2-micron DNA in Saccharomyces cerevisiae. This multiple copy plasmid, organized into chromatin structure in vivo, probably exists in the nucleus and provides a good system to obtain information on eukaryotic DNA replication.
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Phytoplasma Plasmid DNA Extraction
2012Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl-bisbenzimide gradients that exploit the low G+C content of ...
Mark T, Andersen, Lia W, Liefting
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Microbes and Infection, 2005
Plasmid DNA vaccination against tuberculosis is a very powerful and easy method for the induction of strong humoral responses, CD4+ mediated secretion of Th1 cytokines and CD8+ mediated CTL activity in mice. Tuberculosis DNA vaccines have not been assessed so far in humans, and clinical trials with DNA in general have been somewhat disappointing ...
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Plasmid DNA vaccination against tuberculosis is a very powerful and easy method for the induction of strong humoral responses, CD4+ mediated secretion of Th1 cytokines and CD8+ mediated CTL activity in mice. Tuberculosis DNA vaccines have not been assessed so far in humans, and clinical trials with DNA in general have been somewhat disappointing ...
openaire +2 more sources