Results 21 to 30 of about 982,599 (302)

Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]

, 1987
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
A Crossway, A Deshayes, B Reiss, BR Thomas, C-L Hsiao, CP Meredith, DT Stinchcomb, EA Shahin, FA Krens, G Scangos, HC Birnboim, HE Ruley, I Potrykus, J Messing, J Paszkowski, Jacques Hille, JD Rochaix, K Struhl, K Wernars, KC Reed, KR Folger, M Koornneef, M Perucho, Maarten Jongsma, Maarten Koornneef, MG Murray, MW Bevan, O Smithies, P Zabel, Pim Zabel, PW Rigby, R Hain, R Peerbolte, RA Anderson, RD Shillito, S Kato, SN Cohen, T Maniatis, TL Adams   +38 more
core   +4 more sources

Endocytosis of DNA-Hsp65 alters the pH of the late endosome/lysosome and interferes with antigen presentation. [PDF]

PLoS ONE, 2007
BACKGROUND: Experimental models using DNA vaccine has shown that this vaccine is efficient in generating humoral and cellular immune responses to a wide variety of DNA-derived antigens.
Ana Paula F Trombone, Célio L Silva, Karla M Lima, Constance Oliver, Maria Célia Jamur, Alan R Prescott, Arlete A M Coelho-Castelo   +6 more
doaj   +1 more source

A one-step miniprep for the isolation of plasmid DNA and lambda phage particles. [PDF]

PLoS ONE, 2011
Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format.
George Lezin, Yasuhiro Kosaka, H Joseph Yost, Michael R Kuehn, Luca Brunelli   +4 more
doaj   +1 more source

Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensis

Bio-Protocol, 2023
High yield of good quality plasmid DNA from gram -ve bacteria (Agrobacterium tumefaciens, A. rhizogenes, and Rhizobium sp.) and gram +ve bacterium (Bacillus thuringiensis) is difficult. The widely used plasmid extraction kits for Escherichia coli yield a
Preshobha Kodackattumannil, Shina Sasi, Saranya Krishnan, Geetha Lekshmi, Martin Kottackal, Khaled Amiri   +5 more
doaj   +1 more source

Multiple pathways of plasmid DNA transfer in Helicobacter pylori [PDF]

, 2012
Many Helicobacter pylori (Hp) strains carry cryptic plasmids of different size and gene content, the function of which is not well understood. A subgroup of these plasmids (e.g.
Benghezal, Mohammed, Fischer, Wolfgang, Haas, Rainer, Holsten, Lea, Rohrer, Stefanie, Weiss, Evelyn   +5 more
core   +3 more sources

A New Technique With Calcium Phosphate Precipitate Enhances Efficiency of In Vivo Plasmid DNA Gene Transfer

Journal of Pharmacological Sciences, 2005
In vivo gene transfer with plasmid vector has been applied experimentally and clinically; however, the low level of gene transfer efficiency with plasmid vector is a problem.
Shinji Kuroda, Hisatomo Kondo, Keiichi Ohya, Shohei Kasugai   +3 more
doaj   +1 more source

Scientific advice on the suitability of data for the assessment of DNA integration into the fish genome of a genetically modified DNA plasmid-based veterinary vaccine [PDF]

EFSA Journal, 2013
Pancreas disease caused by salmonid alphavirus in farmed Atlantic salmon (Salmo salar) leads to high mortality rates post infection and histopathological lesions in several organs. As protection against pancreas disease, Novartis developed a prophylactic
European Food Safety Authority
doaj   +1 more source

Evidence that the 36kb plasmid of Brachyspira hyodysenteriae contributes to virulence [PDF]

, 2011
Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Recently the genome of virulent Australian B. hyodysenteriae strain WA1 was sequenced, and a 36. kilobase (kb)
Bellgard, M.I., Hampson, D.J., La, T., O'Hara, A.J., Phillips, N.D., Wanchanthuek, P.   +5 more
core   +2 more sources

Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

Synthetic and Systems Biotechnology, 2019
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories.
Lorna Suckling, Ciaran McFarlane, Chelsea Sawyer, Stephen P. Chambers, Richard I. Kitney, David W. McClymont, Paul S. Freemont   +6 more
doaj   +1 more source

Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells. [PDF]

PLoS Genetics, 2009
Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA.
Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann   +7 more
doaj   +1 more source