Results 31 to 40 of about 982,599 (302)
Transformation of the Methylotrophic Actinomycete Amycolatopis methanolica with Plasmid DNA: Stimulatory Effect of a pMEA300-Encoded Gene [PDF]
, 1995 Amycolatopsis methanolica contains a 13.29-kb plasmid (pMEA300) present both in the free state and integrated at a unique genomic location. A pMEA300-free derivative (strain WV1) was selected, allowing further analysis of pMEA300-encoded functions. Whole
Dijkhuizen, L., +2 more
core +2 more sources
Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]
, 2013 The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore +54 more
core +1 more source
On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]
, 1976 An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM +7 more
core +2 more sources
Plasmid DNA-Based Alphavirus Vaccines [PDF]
Vaccines, 2019 Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines, primary cell cultures, and in vivo.
openaire +3 more sources
Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR. [PDF]
PLoS ONE, 2017 Bacteria can be considered as biological nanofactories that manufacture a cornucopia of bioproducts most notably recombinant proteins. As such, they must perfectly match with appropriate plasmid vectors to ensure successful overexpression of target genes.
Magdalena Plotka +2 more
doaj +1 more source
Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems [PDF]
, 2013 Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo ...
Gruber, C.J. +7 more
core +1 more source
Topological Behavior of Plasmid DNA [PDF]
Microbiology Spectrum, 2014 ABSTRACT The discovery of the B-form structure of DNA by Watson and Crick led to an explosion of research on nucleic acids in the fields of biochemistry, biophysics, and genetics. Powerful techniques were developed to reveal a myriad of different structural conformations that change B-DNA as it is transcribed, replicated, and ...
N Patrick, Higgins +1 more
openaire +2 more sources
Polymer induced condensation of dna supercoils [PDF]
, 2008 Macromolecular crowding is thought to be a significant factor driving DNA condensation in prokaryotic cells. Whereas DNA in prokaryotes is supercoiled, studies on crowding-induced DNA condensation have so far focused on linear DNA.
Bessa Ramos Jr., J.E. +2 more
core +2 more sources
Carbon-nanotube for Transient Expression in Rice Calli
Hayati Journal of Biosciences, 2022 Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency.
Paphawarin Pinyokham +5 more
doaj +1 more source
Vaccines, 2023 DNA integrity is a key issue in gene therapy and genetic vaccine approaches based on plasmid DNA. In contrast to messenger RNA that requires a controlled cold chain for efficacy, DNA molecules are considered to be more stable.
Federico D’Alessio +11 more
doaj +1 more source