Results 251 to 260 of about 101,655 (293)

Activation of Val442-plasminogen (mini-plasminogen) by urokinase, streptokinase and tissue plasminogen activator

Thrombosis Research, 1988
Glu-plasminogen (Glu-plg), Lys-plg and Val442-plg (mini-plg) were activated by urokinase (UK), streptokinase (SK) or tissue plasminogen activator (t-PA). Their activation rates were kinetically analyzed. UK activated Lys-plg with smaller Km and nearly identical Vmax as Glu-plg.
Yoshiaki Sugawara, Akikazu Takada, Yumiko Takada   +2 more
openaire   +2 more sources

Importance of the interaction between plasminogen and fibrin for plasminogen activation by tissue-type plasminogen activator

Thrombosis Research, 1984
The potentiating effect of fibrin monomer on plasminogen activation by tissue-type plasminogen activator is much more important with lys-plasminogen than with mini-plasminogen (which lacks the high affinity lysine-binding site important for binding to fibrin).
Claudine Soria, F. W. Dunn, Jeannette Soria, K. Deguchi, J.P. Caen, H.R. Lijnen, Gérard Tobelem   +6 more
openaire   +3 more sources

Fluorescence polarization assay of plasmin, plasminogen, and plasminogen activator

Analytical Biochemistry, 1980
Abstract We describe two assay methods for plasminogen activator (urokinase) employing the fluorescence polarization technique. One method utilizes fluoroscein isothiocyanate-labeled plasminogen as the substrate for urokinase (direct method), and the other utilizes the new plasmin generated from plasminogen by urokinase, for which fluoroscein ...
Hiroshi Maeda, Yorio Hinuma, Kazuo Kinoshita   +2 more
openaire   +3 more sources

[22] Plasminogen and plasmin

1976
Publisher Summary Plasmin is a serine protase with trypsin-like specificity. It cleaves or hydrolyzes proteins and peptides at arginyl and lysyl peptide bonds, and basic amino acid esters, and amides. Plasmin appears to have a preference for lysyl bonds in both protein and ester substrates.
Kenneth C. Robbins, Louis Summaria
openaire   +2 more sources

Activation of Bovine Plasminogen by the Plasminogen Activator in Animal Tissue

Thrombosis and Haemostasis, 1960
Summary1. The activation of bovine plasminogen by means of the plasminogen activator from pig heart tissue has been studied on heated fibrin and on casein.2. Small, but varying, amounts of activator can be extracted from crude activator powder during the experiment and interfere therefore with estimations on normal, plasminogen containing fibrin.3. The
Takeshi Abe, Tage Astrup
openaire   +3 more sources

Plasminogen activation by tissue plasminogen activator in the presence of platelets

Thrombosis Research, 1988
Platelets were found to provide a surface for activation of plasminogen by the tissue-type plasminogen activator (t-PA) at an optimum concentration and to potentiate the generation of plasmin by the amidolytic method, fibrin lysis time and fibrin plate method.
Katsumi Deguchi, Shigeru Shirakawa
openaire   +3 more sources

Conformational Studies of Human Plasminogen and Plasminogen Fragments: Evidence for a Novel Third Conformation of Plasminogen

Biochemistry, 1994
The conformations of Glu-plasminogen and defined proteolytic fragments, in the presence and absence of 6-aminohexanoic acid (6-AHA), trans-4-(aminomethyl)cyclohexanecarboxylic acid (t-AMCHA), and benzamidine, were studied using three methods: size-exclusion high-performance liquid chromatography (SE-HPLC), small-angle X-ray scattering (SAXS), and ...
Chris P. Ponting, Adrian J. Brown, Julian M. Marshall   +2 more
openaire   +3 more sources

Annexin II: a plasminogen-plasminogen activator co-receptor

Frontiers in Bioscience, 2002
Fibrinolysis is a precisely orchestrated process in which fibrin-containing thrombi are solubilized. Several receptors regulate this process by localizing proteolytic activity to the cell surface. One such receptor is annexin II, a calcium and phospholipid-binding protein.
Katherine A. Hajjar, Jiyun Kim
openaire   +3 more sources

Tissue Activator of Plasminogen

Nature, 1964
SINCE Astrup and Permin1 reported that animal tissue cells contain an activator of plasminogen, various authors have confirmed their results, and comparisons of the activator content of different human2 and animal tissues3,4 have been published. Most authors have used whole tissue or aqueous tissue extracts and have estimated activity by the fibrin ...
Charles H. Lack, S. Yousuf Ali
openaire   +3 more sources

Studies on plasminogen. VII. Mechanism of activation of bovine plasminogen

Canadian Journal of Biochemistry, 1969
Native bovine plasminogen is converted to an altered plasminogen and then to plasmin I by either urokinase, plasmin I, or plasmin II. Plasmin I is converted by self-catalysis to plasmin II. Plasmin II degrades itself to the inactive fragment peptide A.
Edwin T. Mertz, Lynn M. Hoepfinger
openaire   +3 more sources