Results 121 to 130 of about 5,147 (230)

Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes

BioTechniques, 2012
A coupled enzyme assay able to monitor the kinetics of reactions catalyzed by phosphate- or pyrophosphate-releasing enzymes is presented here.
Antonio S. Guillén Suárez, Alessandra Stefan, Silvia Lemma, Emanuele Conte, Alejandro Hochkoeppler   +4 more
doaj   +1 more source

Antibiotic influx and efflux in Pseudomonas aeruginosa: Regulation and therapeutic implications

Microbial Biotechnology, Volume 17, Issue 5, May 2024.
This work comprehensively summarized the regulatory networks of the major porin proteins and efflux pumps that play critical roles in antibiotic influx and efflux in Pseudomonas aeruginosa. It also discussed promising therapeutic approaches to combat multidrug‐resistant P.
Weiyan Wu, Jiahui Huang, Zeling Xu
wiley   +1 more source

Insights into Xanthomonas axonopodis pv. citri biofilm through proteomics [PDF]

, 2013
Background: Xanthomonas axonopodis pv. citri (X. a. pv. citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and
Garavaglia, Betiana Soledad, Gehring, Chris, Gottig Schor, Natalia, Marondedze, Claudius, Ottado, Jorgelina, Thomas, Zimaro, Tamara   +6 more
core   +1 more source

The protein‐only RNase Ps, endonucleases that cleave pre‐tRNA: Biological relevance, molecular architectures, substrate recognition and specificity, and protein interactomes

WIREs RNA, Volume 15, Issue 2, March/April 2024.
Three distinct classes of PRORP evolved to cleave the 5′ leader sequence from pre‐tRNA. Beyond available structure, kinetic, and thermodynamic data, there is more to learn about the protein‐interaction network, substrate specificity, and disease‐linked mutations related to PRORP.
Catherine A. Wilhelm, Kipchumba Kaitany, Abigail Kelly, Matthew Yacoub, Markos Koutmos   +4 more
wiley   +1 more source

Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli

BMC Genomics, 2015
The RNA steady-state levels in the cell are a balance between synthesis and degradation rates. Although transcription is important, RNA processing and turnover are also key factors in the regulation of gene expression. In Escherichia coli there are three
V. Pobre, C. Arraiano
semanticscholar   +1 more source

Erythrocyte metabolism

Acta Physiologica, Volume 240, Issue 3, March 2024.
Abstract Our aim is to present an updated overview of the erythrocyte metabolism highlighting its richness and complexity. We have manually collected and connected the available biochemical pathways and integrated them into a functional metabolic map.
Panagiotis N. Chatzinikolaou, Nikos V. Margaritelis, Vassilis Paschalis, Anastasios A. Theodorou, Ioannis S. Vrabas, Antonios Kyparos, Angelo D'Alessandro, Michalis G. Nikolaidis   +7 more
wiley   +1 more source

Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria

Nature Communications, 2018
G-rich RNAs encoded in mitochondrial DNA are prone to form four-stranded structures called G-quadruplexes (G4s). Here the authors show using in vitro and in vivo approaches that GRSF1 promotes melting of G4 RNA structures in mtRNAs, thus leading to their
Zbigniew Pietras, Magdalena A. Wojcik, Lukasz S. Borowski, Maciej Szewczyk, Tomasz M. Kulinski, Dominik Cysewski, Piotr P. Stepien, Andrzej Dziembowski, Roman J. Szczesny   +8 more
doaj   +1 more source

Oxygen consumption and usage during physical exercise: the balance between oxidative stress and ROS-dependent adaptive signaling. [PDF]

, 2013
The complexity of human DNA has been affected by aerobic metabolism, including endurance exercise and oxygen toxicity. Aerobic endurance exercise could play an important role in the evolution of Homo sapiens, and oxygen was not important just for ...
Atalay, Mustafa, Koltai, Erika, Ohno, Hideki, Radak, Zsolt, Zhao, Zhongfu   +4 more
core   +1 more source

Characterization of the RNase R association with ribosomes [PDF]

, 2014
BACKGROUND: In this study we employed the TAP tag purification method coupled with mass spectrometry analysis to identify proteins that co-purify with Escherichia coli RNase R during exponential growth and after temperature downshift.
Cecilia M Arraiano, Cátia Bárria, Michal Malecki   +2 more
core   +1 more source

Purine nucleoside phosphorylase inhibition ameliorates age-associated lower urinary tract dysfunctions

JCI Insight, 2020
In the aging population, lower urinary tract (LUT) dysfunction is common and often leads to storage and voiding difficulties classified into overlapping symptom syndromes.
Lori A. Birder, Amanda Wolf-Johnston, Alan J. Wein, Fangzhou Cheng, Mara Grove-Sullivan, Anthony J. Kanai, Alan M. Watson, Donna Stoltz, Simon C. Watkins, Anne M. Robertson, Diane Newman, Roger R. Dmochowski, Edwin K. Jackson   +12 more
doaj   +1 more source