Results 131 to 140 of about 1,930 (169)

A possible mechanism for the antiviral activity of pokeweed antiviral protein

Physiological and Molecular Plant Pathology, 1993
Abstract The mechanism by which pokeweed antiviral protein (PAP) inhibits the infection of tobacco by TMV was investigated. The N-glycosidase activity of PAP was measured using a combination of aniline treatment to cleave the rRNA at the depurinated site, gel electrophoresis and Northern blot hybridization using a probe specific for the 3′ end of ...
Zhao-chun Chen, John F. Antoniw, Raymond F. White   +2 more
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Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds

Acta Crystallographica Section D Biological Crystallography, 1998
Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine.
H M, Li, Z H, Zeng, Z, Hu, D C, Wang
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Reversal of the inhibitory effects of the pokeweed antiviral protein upon protein synthesis

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
Protein synthesis directed by natural mRNA is more sensitive to the inhibitory action of the pokeweed antiviral protein than synthesis directed by poly-(uridylic acid). Investigations into the nature of this difference revealed that pokeweed antiviral protein does not inhibit the initiation stage of protein synthesis and that the expression of pokeweed
T L, Rodes, J D, Irvin
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Cytotoxicity of pokeweed antiviral protein.

Cytobios, 1989
Pokeweed antiviral protein, a plant protein which inactivates eukaryotic ribosomes, was found to be cytotoxic to both HeLa and Vero cells. Cellular protein synthesis was inhibited by exposure of the cells to microM concentrations of the antiviral protein for 24 h periods or longer.
G M, Aron, J D, Irvin
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Isolation and analysis of a genomic clone encoding a pokeweed antiviral protein

Plant Molecular Biology, 1992
Partial cDNAs encoding a pokeweed antiviral protein were obtained by polymerase chain reaction from the poly(A)+ RNA of seeds, leaves, and roots using two specific primers based on the amino acid sequence of a pokeweed antiviral protein from the seeds (PAP-S).
J, Kataoka, N, Habuka, C, Masuta, M, Miyano, A, Koiwai   +4 more
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Mucosal Toxicity Studies of a Gel Formulation of Native Pokeweed Antiviral Protein

Toxicologic Pathology, 2004
Pokeweed antiviral protein (PAP), a 29-kDa plant-derived protein isolated from Phytolacca americana, is a promising nonspermicidal broad-spectrum antiviral microbicide. This study evaluated the mucosal toxicity potential of native PAP in the in vivo rabbit vaginal irritation model as well as the in vitro reconstituted human vaginal epithelial tissue ...
Osmond J, D'Cruz, Barbara, Waurzyniak, Fatih M, Uckun   +2 more
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Plant resistance to fungal infection induced by nontoxic pokeweed antiviral protein mutants

Nature Biotechnology, 1997
Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca americana inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. Transgenic plants expressing PAP are resistant to a broad spectrum of plant viruses.
O, Zoubenko, F, Uckun, Y, Hur, I, Chet, N, Tumer   +4 more
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High-Level Expression and Purification of Biologically Active Recombinant Pokeweed Antiviral Protein

Protein Expression and Purification, 1999
Pokeweed antiviral protein (PAP) from the leaves of the pokeweed plant, Phytolacca americana, is a naturally occurring single-chain ribosome-inactivating protein, which catalytically inactivates both prokaryotic and eukaryotic ribosomes. The therapeutic potential of PAP has gained considerable interest in recent years due to the clinical use of native ...
F, Rajamohan, C R, Engstrom, T J, Denton, L A, Engen, I, Kourinov, F M, Uckun   +5 more
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Expression characteristics of pokeweed antiviral proteins (PAPs): Two distinct types of proteins

Journal of Plant Biology, 1997
Pokeweed antiviral proteins (PAPs) become novel therapeutic agents in relation to application in human viral diseases and cancer, as well as potent tools in plant system for defending viral infection. We have studied the expression characteristics of PAPs in pokeweed plants by western blot analysis.
Hur Yoonkang, Ching -Tack Han, Jueson Maeng   +2 more
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Expression of pokeweed (Phytolacca americana) antiviral protein cDNA in Escherichia coli and its antiviral activity

Physiological and Molecular Plant Pathology, 1993
Abstract Two DNA constructs encoding the pokeweed antiviral protein (PAP), one encoding PAP and its signal peptide and the other encoding PAP, were made using polymerase chain reactions (PCR), inserted into the expression vector pKK233-2 and transformed into Escherichia coli JM109 cells.
Zhao-chun Chen, John F. Antoniw, Quan Lin, Raymond F. White   +3 more
openaire   +1 more source