Results 41 to 50 of about 122,513 (306)
The poly(A)‐binding protein facilitates in vitro translation of poly(A)‐rich mRNA [PDF]
To investigate the role of the 73‐kDa poly(A)‐binding protein in protein synthesis, the effect of the addition of homo‐polyribonucleotides on the translation of polyadenylated and non‐adenylated mRNA was studied in the rabbit reticulocyte lysate. Poly(A) was found to be the most effective polynucleotide in inhibiting duck‐globin mRNA translation ...
M F, Grossi de Sa +5 more
openaire +2 more sources
Two homologous EF-G proteins from Pseudomonas aeruginosa exhibit distinct functions. [PDF]
Genes encoding two proteins corresponding to elongation factor G (EF-G) were cloned from Pseudomonas aeruginosa. The proteins encoded by these genes are both members of the EFG I subfamily. The gene encoding one of the forms of EF-G is located in the str
Stephanie O Palmer +4 more
doaj +1 more source
The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro. [PDF]
Using an in vitro mRNA decay system, we investigated how poly(A) and its associated poly(A)-binding protein (PABP) affect mRNA stability. Cell extracts used in the decay reactions were depleted of functional PABP either by adding excess poly(A) competitor or by passing the extracts over a poly(A)-Sepharose column.
P, Bernstein, S W, Peltz, J, Ross
openaire +2 more sources
Pickering emulsions have been widely used for biphasic catalysis in the past decade. However, it remains a great challenge to achieve simple product collection and enzyme recovery.
Xin Guan, Zhili Wan, To Ngai
doaj +1 more source
Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
The localisation of poly(A) RNA in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus, Arabidopsis thaliana) nuclei was studied through in situ hybridisation. In both types of nuclei, the amount of poly(A) RNA was much
Janusz Niedojadło +3 more
doaj +1 more source
Polymersomes, smaller than you think: ferrocene as a TEM probe to determine core structure
By incorporating ferrocene into the hydrophobic membrane of PEG-b-PCL polymersome nanoparticles it is possible to selectively visualize their core using Transmission Electron Microscopy (TEM).
University of Southampton +6 more
core +1 more source
Identification of a Human Cytoplasmic Poly(A) Nuclease Complex Stimulated by Poly(A)-binding Protein [PDF]
The poly(A) tail shortening in mRNA, called deadenylation, is the first rate-limiting step in eukaryotic mRNA turnover, and the polyadenylate-binding protein (PABP) appears to be involved in the regulation of this step. However, the precise role of PABP remains largely unknown in higher eukaryotes.
Naoyuki, Uchida +2 more
openaire +2 more sources
Background Poly-ADP-ribosylation, a reversible post-translational modification of primarily chromosomal proteins, is involved in various cellular and molecular processes including carcinogenesis.
Sharan Rajeshwar N +2 more
doaj +1 more source
Ros A, Hellmich W, Duong TT, Anselmetti D. Towards single molecule analysis in PDMS microdevices: from the detection of ultra low dye concentrations to single DNA molecule studies. Journal of biotechnology. 2004;112(1-2):65-72.In this paper, we report on
Hellmich, Wibke +3 more
core +1 more source
Nanoparticles (NPs) based on amphiphilic block copolymers of polyethylene glycol (PEG) and biodegradable polyesters are of particular current interest in drug nanodelivery due to their easily manipulated properties.
Claudia Conte +7 more
doaj +1 more source

