Results 51 to 60 of about 122,513 (306)

Crosstalk Between SUMO and Ubiquitin-Like Proteins: Implication for Antiviral Defense

Frontiers in Cell and Developmental Biology, 2021
Interferon (IFN) is a crucial first line of defense against viral infection. This cytokine induces the expression of several IFN-Stimulated Genes (ISGs), some of which act as restriction factors.
Mounira K. Chelbi-Alix, Pierre Thibault, Pierre Thibault   +2 more
doaj   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

Molecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute, Madeleine Birgersson, Paolo Ceriani, Margareta Wilhelm, Ourania N Kostopoulou   +4 more
wiley   +1 more source

Galactose starvation in a bloodstream form Trypanosoma brucei UDP-glucose 4'-epimerase conditional null mutant [PDF]

, 2006
Galactose metabolism is essential for the survival of Trypanosoma brucei, the etiological agent of African sleeping sickness. T. brucei hexose transporters are unable to transport galactose, which is instead obtained through the epimerization of UDP ...
Turnock, Daniel C., Ferguson, Michael A. J., Ferguson, Michael A. J.; id_orcid, Urbaniak, Michael D.   +3 more
core   +1 more source

Poly-sarcosine and Poly(Ethylene-Glycol) Interactions with Proteins Investigated Using Molecular Dynamics Simulations

Computational and Structural Biotechnology Journal, 2018
Nanoparticles coated with hydrophilic polymers often show a reduction in unspecific interactions with the biological environment, which improves their biocompatibility.
Giovanni Settanni, Timo Schäfer, Christian Muhl, Matthias Barz, Friederike Schmid   +4 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Construction and screening of a pea root cDNA library [PDF]

, 1988
A cDNA library was synthesized using poly(A)(^+) RNA purified from the total RNA from roots of pea (Pisum sativum L.). Ten representative clones encoding abundant root proteins were isolated from the library after screening using colony hybridization ...
Choy, Andy Kwan Yan
core  

Organic Electrochemical Transistors and Foreign Body Reaction: First Steps Toward Long‐Term Implantable Biosensing

Advanced Electronic Materials
Organic Electrochemical Transistors (OECT) have been widely used to detect a myriad of analytes and signals, ranging from glucose content in sweat to brain epileptiform activity.
Henrique Frulani de Paula Barbosa, Titinun Nuntapramote, Ankush Kumar, Sander van den Driesche, Michael J. Vellekoop, Dorothea Brüggemann, Björn Lüssem   +6 more
doaj   +1 more source

The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes

Genome Biology, 2017
Background All mRNAs are bound in vivo by proteins to form mRNA–protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how
Olivia S. Rissland, Alexander O. Subtelny, Miranda Wang, Andrew Lugowski, Beth Nicholson, John D. Laver, Sachdev S. Sidhu, Craig A. Smibert, Howard D. Lipshitz, David P. Bartel   +9 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source