Predictive modeling of plant messenger RNA polyadenylation sites [PDF]
, 2007 Background: One of the essential processing events during pre-mRNA maturation is the post-transcriptional addition of a polyadenine [ poly( A)] tail. The 3'-end poly( A) track protects mRNA from unregulated degradation, and indicates the integrity of ...
Davis, Kimberly M. +10 more
core +2 more sources
TAIL-seq: Genome-wide Determination of Poly(A) Tail Length and 3′ End Modifications [PDF]
Molecular Cell, 2014 Global investigation of the 3' extremity of mRNA (3'-terminome), despite its importance in gene regulation, has not been feasible due to technical challenges associated with homopolymeric sequences and relative paucity of mRNA. We here develop a method, TAIL-seq, to sequence the very end of mRNA molecules.
Chang, Hyeshik +3 more
openaire +2 more sources
Molecular Oncology, EarlyView.Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova +14 more
wiley +1 more source
Cell ReportsSummary: Without new transcription, gene expression across the oocyte-to-embryo transition (OET) relies instead on regulation of mRNA poly(A) tails to control translation.
Katherine Lee +3 more
doaj +1 more source
Deadenylation of mRNA by the CCR4-NOT complex in Drosophila: molecular and developmental aspects
Frontiers in Genetics, 2014 Controlled shortening of the poly(A) tail of mRNAs is the first step in eukaryotic mRNA decay and can also be used for translational inactivation of mRNAs.
Claudia eTemme +2 more
doaj +1 more source
The role of cytoplasmic polyadenylation element sequence on mRNA abundance in porcine embryogenesis [PDF]
Title from PDF of title page (University of Missouri--Columbia, viewed on June 4, 2010).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the ...
Dobbs, Kyle Bradley, 1986-
core +1 more source
Self-Assembly of Supramolecules Consisting of Octyl Gallate Hydrogen Bonded to Polyisoprene-block-poly(vinylpyridine) Diblock Copolymers [PDF]
, 2004 Synchrotron radiation was used to investigate the self-assembly in two comb-shaped supramolecules systems consisting of octyl gallate (OG), i.e., 1-octyl-3,4,5-trihydroxybenzoate, hydrogen bonded to the pyridine groups of polyisoprene-block-poly ...
Bondzic, S +10 more
core +2 more sources
Targeting TNBC: core–shell polycationic polyurea dendrimers with inherent anticancer activity
FEBS Open Bio, EarlyView.Core–shell polycationic PURE dendrimers were tested in TNBC‐derived tumor models. Both formulations selectively targeted TNBC and effectively reduced tumor volume. PUREG4‐OEI48 suppressed tumor growth without detectable toxicity, whereas PUREG4‐OCEI24, despite showing efficacy, induced hepatic toxicity.
Adriana Cruz +9 more
wiley +1 more source
Depletion of nuclear poly(A) binding protein PABPN1 produces a compensatory response by cytoplasmic PABP4 and PABP5 in cultured human cells. [PDF]
PLoS ONE, 2012 BACKGROUND: In vertebrates, poly(A) binding protein (PABP) is known to exist in five different isoforms. PABPs are primarily cytosolic with the exception of the nuclear PABP (PABPN1), which is located in the nucleus.
Rumpa Biswas Bhattacharjee +1 more
doaj +1 more source
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources