Results 91 to 100 of about 693,927 (396)

Equi‐MOI ratio for rapid baculovirus‐mediated multiprotein co‐expression in insect cells integrating selenomethionine for structural studies

open access: yesFEBS Open Bio, EarlyView.
This study presents a versatile protocol for producing selenomethionine‐labeled multiprotein complexes, addressing challenges in structural biology such as experimental phasing in X‐ray crystallography. Its efficiency and adaptability make it valuable for researchers studying de novo, dynamic, or complex protein structures, promoting advancements in ...
Andrej Bitala   +3 more
wiley   +1 more source

Silver-staining of proteins in polyacrylamide gels: a general overview [PDF]

open access: yesCellular and Molecular Biology (Noisy-le-Grand, France) 40, 1 (1994) 57-75, 2009
On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested.
arxiv  

Epitope Mapping of Anti‐Neurofascin 155 Antibody in a Large Cohort of Autoimmune Nodopathy Patients

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Autoimmune nodopathy (AN), a newly recognized disease entity, is an immune‐mediated polyneuropathy involving autoantibodies against cell adhesion molecules located in nodes of Ranvier and paranodal regions, such as neurofascin 186 (NF186) and neurofascin 155 (NF155). The present study aimed to identify the epitopes for autoantibodies
Amina A. Abdelhadi   +6 more
wiley   +1 more source

GENETIC DIFFERENTIATION OF COTTON CULTIVARS BY POLYACRYLAMIDE GEL ELECTROPHORESIS [PDF]

open access: yesJournal of Central European Agriculture, 2005
Information about genetic diversity and relationships among the breeding materials has a signifi cant impact on crop improvement. Association between parental divergence and progeny performance has not been well documented in cotton.
NAVEED Murtaza   +2 more
doaj  

Replacing Acid-Heat Precipitation with Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis Improves Detecting Bence-Jones proteinuria [PDF]

open access: yes, 2019
Background: Detecting Bence-Jones protein in urine is essential for determining plasma cell dyscrasia and multiple myeloma. Conventionally, acid-heat precipitation assay is used for detecting Bence-jones protein in most medical laboratories; however ...
amirzargar, aliakbar
core   +2 more sources

Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis

open access: yesJournal of Virology, 1968
Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis.
A. Shatkin, J. Sipe, P. Loh
semanticscholar   +1 more source

Spider Silk: Rapid, Bottom‐Up Self‐Assembly of MaSp1 into Hierarchically Structured Fibers Through Biomimetic Processing

open access: yesAdvanced Functional Materials, EarlyView.
Spider dragline silk has extraordinary mechanical properties due to the underlying hierarchical organization of the fiber across multiple length scales. A biomimetic platform for MaSp1 is developed, the main protein component of dragline silk, and thereby elucidates the parameters governing its rapid self‐assembly from soluble protein into complex ...
Ali D. Malay   +3 more
wiley   +1 more source

Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1973
A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison
L. R. Orrick, M. Olson, H. Busch
semanticscholar   +1 more source

A versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins [PDF]

open access: yesMethods in molecular biology (Clifton, N.J.) 355 (2006) 111-20, 2006
A new, versatile, multiphasic buffer system for high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins in the relative molecular weight range of 300 000-3000 Da is described. The system, based on the theory of multiphasic zone electrophoresis, allows complete stacking and destacking of proteins in the above M(r) range ...
arxiv  

Sustainable Spinning of Artificial Spider Silk Fibers with Excellent Toughness and Inherent Potential for Functionalization

open access: yesAdvanced Functional Materials, EarlyView.
A sustainable aqueous wet spinning method enables an efficient production of artificial spider silk fibers which show excellent extensibility and remarkable toughness. A highly specific and efficient biomolecular click reaction is employed to functionalize these fibers under mild conditions, which offers a promising method to construct multi‐functional
Ruxia Fan   +6 more
wiley   +1 more source

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