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Development of Dimethylsulfonium Probes for Broad Profiling of Methyllysine Reader Proteins
Development of oligoglycine‐based dimethylsulfonium probes for unbiased crosslinking to methyllysine readers. The general probe facilitates profiling of site‐specific methyllysine readers, evaluation of selectivity and activity of reader inhibitors, and global profiling of methyllysine readers.
Jinyu Yang +3 more
wiley +1 more source
MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection
MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani +6 more
wiley +1 more source
Electrophoretic Profile of Urinary Proteins in Goats During the Peripartum Period. [PDF]
Teklehaymanot BG +8 more
europepmc +1 more source
Brief guide to western blot assays. [PDF]
Ha SG, Park JH, Kim MY, Lee SV.
europepmc +1 more source
Advancing 2-DE Techniques: High-Efficiency Protein Extraction From Lupine Roots. [PDF]
Burchardt S +4 more
europepmc +1 more source
Correction to "Utilization of Fish Byproduct in Edible Films: A Sustainable Approach to Improving Fillet Shelf Life". [PDF]
europepmc +1 more source
Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms.
Fomitcheva Khartchenko A +2 more
europepmc +1 more source
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Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
A, Chrambach, D, Rodbard
openaire +2 more sources
High concentration polyacrylamide gel electrophoresis
Analytical Biochemistry, 1986A simple technique is described for easy removal of high concentration polyacrylamide gels after electrophoresis from glass tubes without breaking them.
V S, Dhamankar +2 more
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