Results 61 to 70 of about 525,236 (336)

A role for glyceraldehyde-3-phosphate dehydrogenase in the development of thermotolerance in Xenopus laevis embryos [PDF]

, 1988
During heat shock, Xenopus laevis embryos exhibit an increase in the rate of accumulation of lactate and a loss of ATP relative to non-heat- shocked control embryos.
Browder, Leon W., Nickells, Robert W.
core   +2 more sources

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

Elution of proteins from polyacrylamide eels: a simple and economic procedure

Revista do Instituto de Medicina Tropical de São Paulo, 1989
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
doaj   +1 more source

Biologic activity in a fragment of recombinant human interferon α [PDF]

, 1984
To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-α2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-α2-(1-110) and ...
Ackerman, Samuel K., Heintzelman, Mark, Hunkapiller, Michael, Zoon, Kathryn, Zur Nedden, Dorothy   +4 more
core  

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Biochemical genetic polymorphism in the Indian mackerel Rastrelliger kanagurta from Mangalore region [PDF]

, 1994
Electrophoresis is the modern and most popular technique used for studying the genetic variability within and among the populations of plants and animals. Genetically controlled tissue enzymes are the most suitable parameters for genetic variability
George, M K, Gupta, I D, Thomas, P C, Verma, N K   +3 more
core  

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Evaluation of arboviruses of public health interest in free-living non-human primates (Alouatta spp., Callithrix spp., Sapajus spp.) in Brazil

Revista da Sociedade Brasileira de Medicina Tropical, 2015
INTRODUCTION: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso
Tatiana Carneiro da Rocha, Paulo Mira Batista, Renato Andreotti, Ana Caroline Dalla Bona, Mário Antônio Navarro da Silva, Rogério Lange, Walfrido Kühl Svoboda, Eliane Carneiro Gomes   +7 more
doaj   +1 more source

Comparative Analysis of the Major Polypeptides from Liver Gap Junctions and Lens Fiber Junctions [PDF]

, 1982
Gap junctions from rat liver and fiber junctions from bovine lens have similar septilaminar profiles when examined by thin-section electron microscopy and differ only slightly with respect to the packing of intramembrane particles in freeze-fracture ...
Anderson, David J., Friedlander, Martin, Gilula, Norton B., Hertzberg, Elliot L.   +3 more
core   +2 more sources