Results 251 to 260 of about 65,820 (278)

The biochemistry of polyadenylation

Trends in Biochemical Sciences, 1996
During the synthesis of mRNA in the nucleus, 3'-ends are generated by endonucleolytic cleavage followed by polyadenylation. The machinery responsible for this simple reaction is surprisingly complex. In vitro reconstitution of 3'-end processing has demonstrated the importance of cooperative interactions in RNA recognition and catalysis.
Wahle, E., Keller, W.
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Cleavage of the polyadenylate-rich region of polyadenylate-rich RNA

Biochemical and Biophysical Research Communications, 1972
Abstract The polyadenylate-rich portion of rapidly-labeled polyribosomal RNA isolated from lymphocytes or adrenal cortex was cleaved by a ribonuclease co-purifying with this RNA and retaining activity despite exposure to SDS and phenol. A ribosomal factor which inhibited the digestion of polyriboadenylate-rich RNA by the associated ribonuclease was ...
Michael G. Rosenfeld, Lucille A. Perkins, Itamar B. Abrass   +2 more
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Cytoplasmic Polyadenylation Assays

2014
Basic research in Drosophila melanogaster has benefited from a plethora of powerful genetics tools. Detailed biochemical analysis, however, has often been difficult due to the lack of in vitro systems that faithfully recapitulate the observations made in vivo.
Fátima Gebauer, Olga Coll, Ana Villalba
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Polyadenylation in Bacteria and Organelles

2014
Polyadenylation is a posttranscriptional modification present throughout all the kingdoms of life with important roles in regulation of RNA stability, translation, and quality control. Functions of polyadenylation in prokaryotic and organellar RNA metabolism are still not fully characterized, and poly(A) tails appear to play contrasting roles in ...
Michal Minczuk, Sarah F. Pearce, Joanna Rorbach, Agnieszka Bobrowicz   +3 more
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Aptamer-Mediated Control of Polyadenylation for Gene Expression Regulation in Mammalian Cells.

ACS Synthetic Biology, 2020
Small aptamer-based regulatory devices can be designed to control a range of RNA-dependent cellular processes and emerged as promising tools for fine-tuning gene expression in synthetic biology.
Maike Spöring, Ronja Boneberg, J. Hartig   +2 more
semanticscholar   +1 more source

Regulation of Retroviral Polyadenylation

2013
Cellular and viral preRNAs are extensively cotranscriptionally modified. These modifications include the processing of the 3' end. Most preRNAs are polyadenylated, which is required for nuclear export, RNA stability, and efficient translation. Integrated retroviral genomes are flanked by 3' and 5' long terminal repeats (LTRs).
Jochen Bodem, A Schuch, Eva-Maria Schrom, Rebecca Moschall   +3 more
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POLYADENYLATION OF mRNA IN PROKARYOTES

Annual Review of Biochemistry, 1997
The 3′-ends of both prokaryotic and eukaryotic mRNA are polyadenylated, but the poly(A) tracts of prokaryotic mRNA are generally shorter, ranging from 15 to 60 adenylate residues and associated with only 2–60% of the molecules of a given mRNA species.
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Developmental profile of polyadenylated and non-polyadenylated GABAA receptor subunit mRNAs

Molecular Brain Research, 1992
The ratio of mRNA not selected for polyadenylation (non-poly(A)+ selected) to mRNA selected for polyadenylation (poly(A)+) for the beta 1, alpha 1 and gamma 2 subunits of the GABAA receptor complex was examined in rats as a function of age. RNA was extracted from whole brain of rats that were either 0, 1, 3, 5 or over 60 days of postnatal age. Poly(A)+
Renee J. Primus, Dorothy W. Gallager, Alicia A. Jacobs   +2 more
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Stability of polyadenylic and polyadenylated ribonucleic acids in radish (Raphanus sativus) seedlings

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
The stability of polyadenylic acid and polyadenylated RNA was investigated in young radish (Raphanus sativus) seedlings. We first studied the decay of poly(A) content, using a [3H]poly(U) assay, following a complete block of transcription by cordycepin (200 microgram/ml). Two lifetime classes of polyadenylic acid have been determined in these seedlings:
Richard Cooke, Lorette Aspart, Michel Delseny   +2 more
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Evaluation of a novel short polyadenylation signal as an alternative to the SV40 polyadenylation signal

Plasmid, 2006
The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown.
Trevor J. McFarland, J. Timothy Stout, Binoy Appukuttan, Y. Zhang, La-ongsri Atchaneeyaskul, Peter J. Francis   +5 more
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