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Cytoplasmic Polyadenylation Assays

2014
Basic research in Drosophila melanogaster has benefited from a plethora of powerful genetics tools. Detailed biochemical analysis, however, has often been difficult due to the lack of in vitro systems that faithfully recapitulate the observations made in vivo.
Fátima Gebauer, Olga Coll, Ana Villalba
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Polyadenylation in Bacteria and Organelles

2014
Polyadenylation is a posttranscriptional modification present throughout all the kingdoms of life with important roles in regulation of RNA stability, translation, and quality control. Functions of polyadenylation in prokaryotic and organellar RNA metabolism are still not fully characterized, and poly(A) tails appear to play contrasting roles in ...
Michal Minczuk   +3 more
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Regulation of Retroviral Polyadenylation

2013
Cellular and viral preRNAs are extensively cotranscriptionally modified. These modifications include the processing of the 3' end. Most preRNAs are polyadenylated, which is required for nuclear export, RNA stability, and efficient translation. Integrated retroviral genomes are flanked by 3' and 5' long terminal repeats (LTRs).
Jochen Bodem   +3 more
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POLYADENYLATION OF mRNA IN PROKARYOTES

Annual Review of Biochemistry, 1997
The 3′-ends of both prokaryotic and eukaryotic mRNA are polyadenylated, but the poly(A) tracts of prokaryotic mRNA are generally shorter, ranging from 15 to 60 adenylate residues and associated with only 2–60% of the molecules of a given mRNA species.
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Stability of polyadenylic and polyadenylated ribonucleic acids in radish (Raphanus sativus) seedlings

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
The stability of polyadenylic acid and polyadenylated RNA was investigated in young radish (Raphanus sativus) seedlings. We first studied the decay of poly(A) content, using a [3H]poly(U) assay, following a complete block of transcription by cordycepin (200 microgram/ml). Two lifetime classes of polyadenylic acid have been determined in these seedlings:
Richard Cooke   +2 more
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Developmental profile of polyadenylated and non-polyadenylated GABAA receptor subunit mRNAs

Molecular Brain Research, 1992
The ratio of mRNA not selected for polyadenylation (non-poly(A)+ selected) to mRNA selected for polyadenylation (poly(A)+) for the beta 1, alpha 1 and gamma 2 subunits of the GABAA receptor complex was examined in rats as a function of age. RNA was extracted from whole brain of rats that were either 0, 1, 3, 5 or over 60 days of postnatal age. Poly(A)+
Renee J. Primus   +2 more
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Evaluation of a novel short polyadenylation signal as an alternative to the SV40 polyadenylation signal

Plasmid, 2006
The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown.
Trevor J. McFarland   +5 more
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The isolation of polyadenylate with unreacted cellulose

Biochemical and Biophysical Research Communications, 1972
Abstract Cellulose, unesterified with polydeoxythymidylate, polyuridylate or acetate selectively binds polyadenylate (poly [A]) and poly [A]- containing RNAs in high salt solution or in low salt solution at pH values of 5.5 or less. It does not bind heteropolymeric RNA but does have some affinity for DNA, particularly in its single-stranded form ...
P.A. Kitos, G. Saxon, Harold Amos
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Bacteriophage T7 mRNA is polyadenylated

Molecular Microbiology, 1998
To determine whether the RNA of bacterial viruses is polyadenylated like bacterial mRNAs, pulse‐labelled as well as the steady‐state population of bacteriophage T7‐specific transcripts were examined for the presence of poly(A) tracts by binding to oligo(dT) cellulose followed by hybridization with specific gene probes. Representatives of all classes of
J. Popowski   +6 more
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In Vivo Analysis of Polyadenylation in Prokaryotes [PDF]

open access: possible, 2014
Polyadenylation at the 3' ends of mRNAs, tRNAs, rRNAs, and sRNAs plays important roles in RNA metabolism in both prokaryotes and eukaryotes. However, the nature of poly(A) tails in prokaryotes is distinct compared to their eukaryotic counterparts. Specifically, depending on the organism, eukaryotic poly(A) tails average between 50 and >200 nt and can ...
Sidney R. Kushner, Bijoy K. Mohanty
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