Results 51 to 60 of about 65,820 (278)

Cdk12 regulates DNA repair genes by suppressing intronic polyadenylation

Nature, 2018
Mutations that attenuate homologous recombination (HR)-mediated repair promote tumorigenesis and sensitize cells to chemotherapeutics that cause replication fork collapse, a phenotype known as ‘BRCAness’1.
S. Dubbury, P. Boutz, P. Sharp
semanticscholar   +1 more source

A novel cause of DKC1‐related bone marrow failure: Partial deletion of the 3′ untranslated region

eJHaem, 2021
Telomere biology disorders (TBDs), including dyskeratosis congenita (DC), are a group of rare inherited diseases characterized by very short telomeres. Mutations in the components of the enzyme telomerase can lead to insufficient telomere maintenance in ...
Jonathan W. Arthur, Hilda A. Pickett, Pasquale M. Barbaro, Tatjana Kilo, Raja S. Vasireddy, Traude H. Beilharz, David R. Powell, Emma L. Hackett, Bruce Bennetts, Julie A. Curtin, Kristi Jones, John Christodoulou, Roger R. Reddel, Juliana Teo, Tracy M. Bryan   +14 more
doaj   +1 more source

RNA binding protein Caprin-2 is a pivotal regulator of the central osmotic defense response

eLife, 2015
In response to an osmotic challenge, the synthesis of the antidiuretic hormone arginine vasopressin (AVP) increases in the hypothalamus, and this is accompanied by extension of the 3′ poly(A) tail of the AVP mRNA, and the up-regulation of the expression ...
Agnieszka Konopacka, Mingkwan Greenwood, Su-Yi Loh, Julian Paton, David Murphy   +4 more
doaj   +1 more source

Reconstitution of CPSF active in polyadenylation: recognition of the polyadenylation signal by WDR33

Genes & Development, 2014
Cleavage and polyadenylation specificity factor (CPSF) is the central component of the 3′ processing machinery for polyadenylated mRNAs in metazoans: CPSF recognizes the polyadenylation signal AAUAAA, providing sequence specificity in both pre-mRNA cleavage and polyadenylation, and catalyzes pre-mRNA cleavage.
Schönemann, Lars, Kühn, Uwe, Martin, Georges, Schäfer, Peter, Gruber, Andreas R., Keller, Walter, Zavolan, Mihaela, Wahle, Elmar   +7 more
openaire   +5 more sources

Alternative polyadenylation of cyclooxygenase-2 [PDF]

Nucleic Acids Research, 2005
A biologically important human gene, cyclooxygenase-2 (COX-2), has been proposed to be regulated at many levels. While COX-1 is constitutively expressed in cells, COX-2 is inducible and is upregulated in response to many signals. Since increased transcriptional activity accounts for only part of the upregulation of COX-2, we chose to explore other RNA ...
Carol S. Lutz, Haibo Zhang, Tyra Hall-Pogar, Bin Tian   +3 more
openaire   +3 more sources

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Detection of Differentially Expressed Cleavage Site Intervals Within 3′ Untranslated Regions Using CSI-UTR Reveals Regulated Interaction Motifs

Frontiers in Genetics, 2019
The length of untranslated regions at the 3′ end of transcripts (3′UTRs) is regulated by alternate polyadenylation (APA). 3′UTRs contain regions that harbor binding motifs for regulatory molecules. However, the mechanisms that coordinate the 3′UTR length
Benjamin J. Harrison, Benjamin J. Harrison, Benjamin J. Harrison, Juw Won Park, Juw Won Park, Cynthia Gomes, Jeffrey C. Petruska, Jeffrey C. Petruska, Jeffrey C. Petruska, Matthew R. Sapio, Michael J. Iadarola, Julia H. Chariker, Julia H. Chariker, Eric C. Rouchka, Eric C. Rouchka   +14 more
doaj   +1 more source

Unraveling LINE‐1 retrotransposition in head and neck squamous cell carcinoma

Molecular Oncology, EarlyView.
The novel RetroTest method allows the detection of L1 activation in clinical samples with low DNA input, providing global L1 activity and the identification of the L1 source element. We applied RetroTest to a real‐world cohort of HNSCC patients where we reported an early L1 activation, with more than 60% of T1 patients showing L1 activity.
Jenifer Brea‐Iglesias, Ana Oitabén, Sonia Zumalave, Bernardo Rodriguez‐Martin, María Gallardo‐Gómez, Martín Santamarina, Ana Pequeño‐Valtierra, Laura Juaneda‐Magdalena, Ramón García‐Escudero, José Luis López‐Cedrún, Máximo Fraga, José M. C. Tubio, Mónica Martínez‐Fernández   +12 more
wiley   +1 more source

Nuclear Polyadenylate-Binding Protein [PDF]

Molecular and Cellular Biology, 1985
Polyadenylate-binding activity can be detected in eluates from sodium dodecyl sulfate gels by a nitrocellulose filter-binding assay. Nuclear extracts from rat liver show a single peak of binding activity at 50 to 55 kilodaltons; cytoplasmic extracts show a single peak at 70 to 80 kilodaltons, corresponding to a 75-kilodalton protein previously ...
Roger D. Kornberg, Alan B. Sachs
openaire   +3 more sources

QAPA: a new method for the systematic analysis of alternative polyadenylation from RNA-seq data

Genome Biology, 2018
Alternative polyadenylation (APA) affects most mammalian genes. The genome-wide investigation of APA has been hampered by an inability to reliably profile it using conventional RNA-seq. We describe ‘Quantification of APA’ (QAPA), a method that infers APA
Kevin C. H. Ha, B. Blencowe, Q. Morris
semanticscholar   +1 more source