Results 151 to 160 of about 166,439 (190)

NONTEMPLATE-DEPENDENT POLYMERIZATION PROCESSES: Polyhydroxyalkanoate Synthases as a Paradigm

Annual Review of Biochemistry, 2005
▪ Abstract  This review focuses on nontemplate-dependent polymerases that use water-soluble substrates and convert them into water-insoluble polymers that form granules or inclusions within the cell. The initial part of the review summarizes briefly the current knowledge of polymer formation catalyzed by starch and glycogen synthases, polyphosphate ...
Joanne, Stubbe, Jiamin, Tian, Aimin, He, Anthony J, Sinskey, Adam G, Lawrence, Pinghua, Liu   +5 more
openaire   +2 more sources

Polyhydroxyalkanoate synthase from Bacillus sp. INT005 is composed of PhaC and PhaR

Journal of Bioscience and Bioengineering, 2002
A polyhydroxyalkanoate (PHA) biosynthesis gene locus from Bacillus sp. INT005 strain, which had been isolated from a gas field, was cloned and analyzed at the molecular level. We found that a 3.8-kbp DraI-digested fragment of genomic DNA of Bacillus sp. INT005 conferred PHA-producing ability to Escherichia coli, which was PHA-negative. The DNA fragment
Yasuharu Satoh, Kenji Tajima, Masanobu Munekata   +2 more
exaly   +3 more sources

Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus

Applied Microbiology and Biotechnology, 2015
This review highlights the recent investigations of class IV polyhydroxyalkanoate (PHA) synthases, the newest classification of PHA synthases. Class IV synthases are prevalent in organisms of the Bacillus genus and are composed of a catalytic subunit PhaC (approximately 40 kDa), which has a PhaC box sequence ([GS]-X-C-X-[GA]-G) at the active site, and ...
Takeharu Tsuge, Manami Hyakutake, Kouhei Mizuno   +2 more
openaire   +2 more sources

Polyhydroxyalkanoate production by a novel bacterium Massilia sp. UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene [PDF]

Journal of Bioscience and Bioengineering, 2014
We successfully isolated one microorganism (UMI-21) from Ulva, a green algae that contains starch. The strain UMI-21 can produce polyhydroxyalkanoate (PHA) from starch, maltotriose, or maltose as a sole carbon source.
Xuerong Han, Yasuharu Satoh, Kenji Tajima   +2 more
exaly   +2 more sources

Characterization of the Polyhydroxyalkanoate Synthase Gene Locus of Rhodobacter Sphaeroides

Biotechnology Letters, 1993
The Rhodobacter sphaeroides polyhydroxyalkanoate synthase structural gene (phaCRs) was identified as a 1,806-bp open reading frame. It encoded a PHA synthase of 601 amino acids with a relative molecular mass of 66,742, which exhibited 25.9 to 39.0 % amino acid identity with PHA synthases from other bacteria.
Eilert Hustede, Alexander Steinbüchel
openaire   +1 more source

Regulation of polyhydroxyalkanoate synthases (phaC1 and phaC2) gene expression in Pseudomonas corrugata

Applied Microbiology and Biotechnology, 2006
In this study we examined polyhydroxyalkanoate (PHA) synthases phaC1 and phaC2 gene expression in two strains of Pseudomonas corrugata (Pc) grown in a minimum mineral medium with related (oleic acid and octanoate) or unrelated (glucose) carbon sources.
CONTE E, CATARA, VITTORIA, GRECO S, RUSSO M, ALICATA R, STRANO L, LOMBARDO A, DI SILVESTRO S, CATARA A.   +8 more
openaire   +3 more sources

Characterization of polyhydroxyalkanoate synthases from the marine bacterium Neptunomonas concharum JCM17730

Journal of Biotechnology, 2020
Neptunomonas concharum JCM17730 was isolated from an ark clam sample and characterized as a mesophilic bacterium. The genome of N. concharum JCM17730 contains thirteen genes related to polyhydroxyalkanoates (PHA) metabolism. Three PHA synthase encoding genes were identified, and phylogenetic analysis of enzyme sequences suggested the presence of two ...
Nan Pu, Meng-Ru Wang, Zheng-Jun Li
openaire   +2 more sources

Organization of Polyhydroxyalkanoate Synthase for In Vitro Polymerization as Revealed by Atomic Force Microscopy

Macromolecular Bioscience, 2005
AbstractSummary: Individual polyhydroxyalkanoate synthase molecules from Ralstonia eutropha (PhaCRe) were directly visualized on highly oriented pyrolytic graphite (HOPG) by atomic force microscopy (AFM). PhaCRe molecule was observed as a spherical particle of 2.9 ± 0.4 nm in height and 28 ± 4 nm in width.
Yoshihiro Kikkawa, Masaharu Narike, Tomohiro Hiraishi, Masatoshi Kanesato, Kumar Sudesh, Yoshiharu Doi, Takeharu Tsuge   +6 more
openaire   +2 more sources

Thiolysis of Poly(3-hydroxybutyrate) with Polyhydroxyalkanoate Synthase from Ralstonia eutropha

The Journal of Biochemistry, 2006
Poly(3-hydroxybutyrate) (PHB) is synthesized from 3-hydroxybutyryl-CoA by polyhydroxyalkanoate synthase and hydrolyzed by PHB depolymerase. In this study, we focused on the reverse reaction of polyhydroxyalkanoate synthase, and propose the possibility that PHB can be degraded through a novel process, that is thiolysis of PHB with CoASH ...
Keiichi, Uchino, Terumi, Saito
openaire   +2 more sources

Cloning and characterization of the Methylobacterium extorquens polyhydroxyalkanoic-acid-synthase structural gene

Applied Microbiology and Biotechnology, 1993
A cosmid gene bank of partially EcoRI-digested genomic DNA from Methylobacterium extorquens IBT no. 6 was screened for DNA fragments restoring polyhydroxyalkanoic-acid (PHA) accumulation in the PHA-negative mutant Alkaligenes eutrophus H16 PHB-4. The M.
H E, Valentin, A, Steinbüchel
openaire   +2 more sources