Multi-layered metabolic remodeling of <i>Pseudomonas putida</i> for efficient conversion of lignocellulosic sugars to the precursors of advanced aviation fuel. [PDF]
Metab Eng CommunKang CW +7 more
europepmc +1 more source
Genome-based classification of Halonovum salinarum gen. nov., sp. nov., and Halonovum rutilum sp. nov., two novel halophilic archaea isolated from a solar saltern. [PDF]
BMC MicrobiolHwang CY +4 more
europepmc +1 more source
Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains. [PDF]
STAR ProtocBaur KS +4 more
europepmc +1 more source
Related searches:
Polyhydroxyalkanoate synthase from Bacillus sp. INT005 is composed of PhaC and PhaR
Journal of Bioscience and Bioengineering, 2002A polyhydroxyalkanoate (PHA) biosynthesis gene locus from Bacillus sp. INT005 strain, which had been isolated from a gas field, was cloned and analyzed at the molecular level. We found that a 3.8-kbp DraI-digested fragment of genomic DNA of Bacillus sp. INT005 conferred PHA-producing ability to Escherichia coli, which was PHA-negative. The DNA fragment
Yasuharu, Satoh +3 more
openaire +4 more sources
Discovery of a new polyhydroxyalkanoate synthase from limestone soil through metagenomic approach
Journal of Bioscience and Bioengineering, 2016PHA synthase (PhaC) is the key enzyme in the production of biodegradable plastics known as polyhydroxyalkanoate (PHA). Nevertheless, most of these enzymes are isolated from cultivable bacteria using traditional isolation method. Most of the microorganisms found in nature could not be successfully cultivated due to the lack of knowledge on their growth ...
Yen Teng, Tai +3 more
openaire +4 more sources
We successfully isolated one microorganism (UMI-21) from Ulva, a green algae that contains starch. The strain UMI-21 can produce polyhydroxyalkanoate (PHA) from starch, maltotriose, or maltose as a sole carbon source. Taxonomic studies and 16S rDNA sequence analysis revealed that strain UMI-21 was phylogenetically related to species of the genus ...
Xuerong Han +7 more
openaire +5 more sources
Polyhydroxyalkanoate (PHA) Homeostasis: The Role of the PHA Synthase
ChemInform, 2003AbstractFor Abstract see ChemInform Abstract in Full Text.
JoAnne, Stubbe, Jiamin, Tian
openaire +2 more sources
Detection of polyhydroxyalkanoate synthase activity on a polyacrylamide gel
Analytical Biochemistry, 2009This study presents a method to detect active polyhydroxyalkanoate (PHA) synthase on a polyacrylamide gel that combines the polyhydroxybutyrate (PHB) polymerization reaction with Sudan Black B staining. After separation of the protein samples on a modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the slab gel was submerged ...
Der-Shyan, Sheu +3 more
openaire +2 more sources
Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus
Applied Microbiology and Biotechnology, 2015This review highlights the recent investigations of class IV polyhydroxyalkanoate (PHA) synthases, the newest classification of PHA synthases. Class IV synthases are prevalent in organisms of the Bacillus genus and are composed of a catalytic subunit PhaC (approximately 40 kDa), which has a PhaC box sequence ([GS]-X-C-X-[GA]-G) at the active site, and ...
Takeharu Tsuge +2 more
openaire +2 more sources
NONTEMPLATE-DEPENDENT POLYMERIZATION PROCESSES: Polyhydroxyalkanoate Synthases as a Paradigm
Annual Review of Biochemistry, 2005▪ Abstract This review focuses on nontemplate-dependent polymerases that use water-soluble substrates and convert them into water-insoluble polymers that form granules or inclusions within the cell. The initial part of the review summarizes briefly the current knowledge of polymer formation catalyzed by starch and glycogen synthases, polyphosphate ...
Joanne, Stubbe +5 more
openaire +2 more sources