Results 161 to 170 of about 2,304 (189)

NONTEMPLATE-DEPENDENT POLYMERIZATION PROCESSES: Polyhydroxyalkanoate Synthases as a Paradigm

Annual Review of Biochemistry, 2005
▪ Abstract  This review focuses on nontemplate-dependent polymerases that use water-soluble substrates and convert them into water-insoluble polymers that form granules or inclusions within the cell. The initial part of the review summarizes briefly the current knowledge of polymer formation catalyzed by starch and glycogen synthases, polyphosphate ...
Joanne, Stubbe, Jiamin, Tian, Aimin, He, Anthony J, Sinskey, Adam G, Lawrence, Pinghua, Liu   +5 more
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Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus

Applied Microbiology and Biotechnology, 2015
This review highlights the recent investigations of class IV polyhydroxyalkanoate (PHA) synthases, the newest classification of PHA synthases. Class IV synthases are prevalent in organisms of the Bacillus genus and are composed of a catalytic subunit PhaC (approximately 40 kDa), which has a PhaC box sequence ([GS]-X-C-X-[GA]-G) at the active site, and ...
Takeharu Tsuge, Manami Hyakutake, Kouhei Mizuno   +2 more
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Characterization of polyhydroxyalkanoate synthases from the marine bacterium Neptunomonas concharum JCM17730

Journal of Biotechnology, 2020
Neptunomonas concharum JCM17730 was isolated from an ark clam sample and characterized as a mesophilic bacterium. The genome of N. concharum JCM17730 contains thirteen genes related to polyhydroxyalkanoates (PHA) metabolism. Three PHA synthase encoding genes were identified, and phylogenetic analysis of enzyme sequences suggested the presence of two ...
Nan Pu, Meng-Ru Wang, Zheng-Jun Li
openaire   +2 more sources

Regulation of polyhydroxyalkanoate synthases (phaC1 and phaC2) gene expression in Pseudomonas corrugata

Applied Microbiology and Biotechnology, 2006
In this study we examined polyhydroxyalkanoate (PHA) synthases phaC1 and phaC2 gene expression in two strains of Pseudomonas corrugata (Pc) grown in a minimum mineral medium with related (oleic acid and octanoate) or unrelated (glucose) carbon sources.
CONTE E, CATARA, VITTORIA, GRECO S, RUSSO M, ALICATA R, STRANO L, LOMBARDO A, DI SILVESTRO S, CATARA A.   +8 more
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Cloning, characterization and comparison of the Pseudomonas mendocina polyhydroxyalkanoate synthases PhaC1 and PhaC2

Applied Microbiology and Biotechnology, 2002
This study describes a comparison of the polyhydroxyalkanoate (PHA) synthases PhaC1 and PhaC2 of Pseudomonas mendocina. The P mendocina pha gene locus, encoding two PHA synthase genes [phaC1Pm and phaC2pm flanking a PHA depolymerase gene (phaZ)], was cloned, and the nucleotide sequences of phaC1Pm (1,677 bp), phaZ (1,034 bp), and phaC2pm (1,680 bp ...
S, Hein, J R J, Paletta, A, Steinbüchel
openaire   +2 more sources

Organization of Polyhydroxyalkanoate Synthase for In Vitro Polymerization as Revealed by Atomic Force Microscopy

Macromolecular Bioscience, 2005
AbstractSummary: Individual polyhydroxyalkanoate synthase molecules from Ralstonia eutropha (PhaCRe) were directly visualized on highly oriented pyrolytic graphite (HOPG) by atomic force microscopy (AFM). PhaCRe molecule was observed as a spherical particle of 2.9 ± 0.4 nm in height and 28 ± 4 nm in width.
Yoshihiro Kikkawa, Masaharu Narike, Tomohiro Hiraishi, Masatoshi Kanesato, Kumar Sudesh, Yoshiharu Doi, Takeharu Tsuge   +6 more
openaire   +2 more sources

Thiolysis of Poly(3-hydroxybutyrate) with Polyhydroxyalkanoate Synthase from Ralstonia eutropha

The Journal of Biochemistry, 2006
Poly(3-hydroxybutyrate) (PHB) is synthesized from 3-hydroxybutyryl-CoA by polyhydroxyalkanoate synthase and hydrolyzed by PHB depolymerase. In this study, we focused on the reverse reaction of polyhydroxyalkanoate synthase, and propose the possibility that PHB can be degraded through a novel process, that is thiolysis of PHB with CoASH ...
Keiichi, Uchino, Terumi, Saito
openaire   +2 more sources

Cloning and characterization of the Methylobacterium extorquens polyhydroxyalkanoic-acid-synthase structural gene

Applied Microbiology and Biotechnology, 1993
A cosmid gene bank of partially EcoRI-digested genomic DNA from Methylobacterium extorquens IBT no. 6 was screened for DNA fragments restoring polyhydroxyalkanoic-acid (PHA) accumulation in the PHA-negative mutant Alkaligenes eutrophus H16 PHB-4. The M.
H E, Valentin, A, Steinbüchel
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Natural and engineered polyhydroxyalkanoate (PHA) synthase: key enzyme in biopolyester production

Applied Microbiology and Biotechnology, 2017
With the finite supply of petroleum and increasing concern with environmental issues associated with their harvest and processing, the development of more eco-friendly, sustainable alternative biopolymers that can effectively fill the role of petro-polymers has become a major focus.
Huibin, Zou, Mengxun, Shi, Tongtong, Zhang, Lei, Li, Liangzhi, Li, Mo, Xian   +5 more
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Increased synthesis of poly(3-hydroxydodecanoate) by random mutagenesis of polyhydroxyalkanoate synthase

Applied Microbiology and Biotechnology, 2018
Poly(3-hydroxydodecanoate) [P(3HDD)], a medium-chain-length polyhydroxyalkanoate (PHA), is expected to be used as a novel type of bioplastic characterized by a soft and transparent nature. In this study, to achieve a high yield of P(3HDD), PHA synthase was modified through random mutagenesis of a region of the PHA synthase 1 gene from Pseudomonas ...
Ayaka Hiroe, Suzuran Watanabe, Makoto Kobayashi, Christopher T. Nomura, Takeharu Tsuge   +4 more
openaire   +2 more sources