Results 161 to 170 of about 166,439 (190)

Increased synthesis of poly(3-hydroxydodecanoate) by random mutagenesis of polyhydroxyalkanoate synthase

Applied Microbiology and Biotechnology, 2018
Poly(3-hydroxydodecanoate) [P(3HDD)], a medium-chain-length polyhydroxyalkanoate (PHA), is expected to be used as a novel type of bioplastic characterized by a soft and transparent nature. In this study, to achieve a high yield of P(3HDD), PHA synthase was modified through random mutagenesis of a region of the PHA synthase 1 gene from Pseudomonas ...
Ayaka Hiroe, Suzuran Watanabe, Makoto Kobayashi, Christopher T. Nomura, Takeharu Tsuge   +4 more
openaire   +2 more sources

Cloning, characterization and comparison of the Pseudomonas mendocina polyhydroxyalkanoate synthases PhaC1 and PhaC2

Applied Microbiology and Biotechnology, 2002
This study describes a comparison of the polyhydroxyalkanoate (PHA) synthases PhaC1 and PhaC2 of Pseudomonas mendocina. The P mendocina pha gene locus, encoding two PHA synthase genes [phaC1Pm and phaC2pm flanking a PHA depolymerase gene (phaZ)], was cloned, and the nucleotide sequences of phaC1Pm (1,677 bp), phaZ (1,034 bp), and phaC2pm (1,680 bp ...
S, Hein, J R J, Paletta, A, Steinbüchel
openaire   +2 more sources

Natural and engineered polyhydroxyalkanoate (PHA) synthase: key enzyme in biopolyester production

Applied Microbiology and Biotechnology, 2017
With the finite supply of petroleum and increasing concern with environmental issues associated with their harvest and processing, the development of more eco-friendly, sustainable alternative biopolymers that can effectively fill the role of petro-polymers has become a major focus.
Huibin, Zou, Mengxun, Shi, Tongtong, Zhang, Lei, Li, Liangzhi, Li, Mo, Xian   +5 more
openaire   +2 more sources

Genetics and Biochemistry of Polyhydroxyalkanoate Granule Self-assembly: The Key Role of Polyester Synthases

Biotechnology Letters, 2006
PHAs (polyhydroxyalkanoates = biopolyester) composed of hydroxy fatty acids represent a rather complex class of storage polymers synthesized by various bacteria and archaea and are deposited as water-insoluble cytoplasmic nano-sized inclusions. These spherical particles are composed of a polyester core surrounded by phospholipids and proteins.
openaire   +3 more sources

The polyhydroxyalkanoate (PHA) synthase 1 of Pseudomonas sp. H9 synthesized a 3-hydroxybutyrate-dominant hybrid of short- and medium-chain-length PHA

Enzyme and Microbial Technology, 2021
Der-Shyan Sheu, Yuh-Ling Lee Chen, Houng-Yung Chen   +2 more
exaly  

In Vivo Characterization and Application of the PHA Synthase from Azotobacter vinelandii for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate

Polymers, 2021
Nazalan Najimudin, Kumar Sudesh
exaly  

Evaluation of BP-M-CPF4 polyhydroxyalkanoate (PHA) synthase on the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil using Cupriavidus necator transformants

International Journal of Biological Macromolecules, 2020
Manoj Lakshmanan, Hua Tiang Tan, Toshio Hakoshima   +2 more
exaly  

Identification of regions affecting enzyme activity, substrate binding, dimer stabilization and polyhydroxyalkanoate (PHA) granule morphology in the PHA synthase of Aquitalea sp. USM4

International Journal of Biological Macromolecules, 2021
Toshio Hakoshima, Min Fey Chek, Kumar Sudesh   +2 more
exaly  

Mutant polyhydroxyalkanoate synthase, gene thereof and transformant, and method for producing polyhydroxyalkanoate

2021
KOBAYASHI SHINGO, YOSHIDA SHINICHI, SATO SHUNSUKE, TAOKA NAOAKI   +3 more
openaire   +1 more source

Unveiling the 30‐year mystery of polyhydroxyalkanoate (PHA) synthase

Biotechnology Journal, 2016
openaire   +2 more sources