Lipopolysaccharide Is a 4‑Aminoarabinose Donor to Exogenous Polyisoprenyl Phosphates through the Reverse Reaction of the Enzyme ArnT [PDF]
ACS Omega, 2021 Modification of the lipid A portion of LPS with cationic monosaccharides provides resistance to polymyxins, which are often employed as a last resort to treat multidrug-resistant bacterial infections. Here, we describe the use of fluorescent polyisoprenoids, liquid chromatography-mass spectrometry, and bacterial genetics to probe the activity of ...
Beth A. Scarbrough +4 more
doaj +7 more sources
Regulation of phosphatidylinositol 3–kinase by polyisoprenyl phosphates in neutrophil-mediated tissue injury [PDF]
Journal of Experimental Medicine, 2006 Neutrophils play a central role in host defense, inflammation, and tissue injury. Recent findings indicate a novel role for polyisoprenyl phosphates (PIPPs) as natural down-regulatory signals in neutrophils. The relationship between PIPPs and neutrophil early activating signals, such as phosphoinositides, has not been previously determined.
Caroline Bonnans +2 more
exaly +11 more sources
Novel polyisoprenyl phosphates block phospholipase D and human neutrophil activation in vitro and murine peritoneal inflammation in vivo [PDF]
British Journal of Pharmacology, 2005 Leukocyte production of reactive oxygen species (ROS) is an essential component of the antimicrobial armament mounted during host defense, but when released to the extracellular milieu ROS can also injure host tissues and provoke inflammation. Polyisoprenyl phosphates (PIPPs) are constituents of human leukocyte membranes that regulate pivotal ...
Bruce D Levy +2 more
exaly +8 more sources
Determination of the anomeric configuration of glycosyl esters of nucleoside pyrophosphates and polyisoprenyl phosphates by fast-atom bombardment tandem mass spectrometry [PDF]
Journal of the American Society for Mass Spectrometry, 1996 Collision-induced dissociation of the deprotonated molecules of glycosyl esters of nucleoside pyrophosphates and polyisoprenyl (dolichyl and polyprenyl) phosphates results in distinct fragmentation patterns that depend on cis-trans configuration of the phosphodiester and 2″ (or 2', respectively)-hydroxyl groups of the glycosyl residue. At the collision-
Edmond De Hoffmann +2 more
exaly +5 more sources
Polyisoprenyl phosphates: natural antiinflammatory lipid signals [PDF]
Cellular and Molecular Life Sciences, 2002 Lipoxins (LX) and aspirin-triggered 15-epimer LX are leukocyte-derived eicosanoids generated during host defense that serve as down-regulatory signals. The specific intracellular events that govern cellular responses to inhibitory extracellular signals are of wide interest in order to understand pivotal intracellular events in diseases characterized by
Charles N Serhan, Bruce D Levy
exaly +5 more sources
Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis [PDF]
Nature Communications, 2016 Abstract The attachment of a sugar to a hydrophobic polyisoprenyl carrier is the first step for all extracellular glycosylation processes. The enzymes that perform these reactions, polyisoprenyl-glycosyltransferases (PI-GTs) include dolichol phosphate mannose synthase (DPMS), which generates the mannose donor for ...
Chiara Ardiccioni +2 more
exaly +6 more sources
The synthesis and chemical properties of polyisoprenyl beta-D-mannopyranosyl phosphates.
Journal of Biological Chemistry, 1975 2,3,4,6-Tetra-O-acetyl-beta-D-mannopyranosyl phosphate, free of the alpha anomer, was coupled with citronellol and dolichol in the presence of triisopropylbenzenesulfonyl chloride to give, after chromatographic purification and deacetylation, the respective polyisoprenyl beta-D-mannopyranosyl phosphates.
A Herscovics
exaly +4 more sources
Structural insights into polyisoprenyl-binding glycosyltransferases [PDF]
StructureGlycosyltransferases (GTs) catalyze the addition of sugars to diverse substrates facilitating complex glycoconjugate biosynthesis across all domains of life.
Ryan T Morgan +2 more
exaly +3 more sources
iScience, 2022 Summary: Lipid phosphate phosphatases are a family of enzymes with diverse cellular metabolic functions. Phospholipid phosphatase 6 (PLPP6) is a regulator of cellular polyisoprenyl phosphates; however, its in vivo functions remain to be determined. Here,
Thayse R. Brüggemann +11 more
doaj +2 more sources
Topological analysis of the Escherichia coli WcaJ protein reveals a new conserved configuration for the polyisoprenyl-phosphate hexose-1-phosphate transferase family [PDF]
Scientific Reports, 2015 AbstractWcaJ is an Escherichia coli membrane enzyme catalysing the biosynthesis of undecaprenyl-diphosphate-glucose, the first step in the assembly of colanic acid exopolysaccharide. WcaJ belongs to a large family of polyisoprenyl-phosphate hexose-1-phosphate transferases (PHPTs) sharing a similar predicted topology consisting of an N-terminal domain ...
Furlong, Sarah E. +3 more
openaire +5 more sources