Gating mechanism of elongating β-ketoacyl-ACP synthases [PDF]
Nature Communications, 2020 The formation of C-C bonds in fatty acid and polyketide biosynthesis depends on β-ketoacyl-acyl carrier protein (ACP) synthases (KSs). Here, the authors present structures of E.coli KSs bound to substrate mimetic bearing ACPs, providing insights into the
Jeffrey T. Mindrebo +9 more
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Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis [PDF]
Nature Communications, 2022 Cocaine is a tropane alkaloid produced by Erythroxylum novogranatense. Here the authors identify two polyketide synthases involved in cocaine biosynthesis and provide insight into the parallel evolution of these enzymes in tropane alkaloids-producing ...
Tian Tian +9 more
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Genomic mining and diversity of assembly line polyketide synthases [PDF]
Open Biology, 2023 Assembly line polyketide synthases (PKSs) are a large family of multifunctional enzymes responsible for synthesizing many medicinally relevant natural products with remarkable structural variety and biological activity.
Shreya Kishore, Chaitan Khosla
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Gene conversion-associated successive engineering of modular polyketide synthases [PDF]
Communications ChemistryModular polyketide synthases (PKSs) can produce various secondary metabolites in a collinearity fashion. Although rational engineering of modular PKS can ultimately create a diverse array of compounds, de novo generation of defined structures usually ...
Wenzheng Jin +4 more
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Basidiomycete non-reducing polyketide synthases function independently of SAT domains [PDF]
Fungal Biology and Biotechnology, 2023 Background Non-reducing polyketide synthases (NR-PKSs) account for a major share of natural product diversity produced by both Asco- and Basidiomycota. The present evolutionary diversification into eleven clades further underscores the relevance of these
Nikolai A. Löhr +5 more
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An in vitro platform for engineering and harnessing modular polyketide synthases [PDF]
Nature Communications, 2020 A robust platform to study modular polyketide synthases (PKSs) in vitro is still unavailable. Here, the authors report the reconstitution of the venemycin PKS, engineer hybrid venemycin/pikromycin PKSs, and obtain much improved yields through employing ...
Takeshi Miyazawa +3 more
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Diversity and Distribution of Non-Reducing Polyketide Synthases (NR-PKSs) in Ascomycota (Fungi) [PDF]
Journal of Fungi(1) Background: This study highlights the diversity and distribution of non-reducing polyketide synthases (NR-PKSs) in Ascomycota and their role in producing bioactive aromatic polyketides.
Pritam Chattopadhyay, Goutam Banerjee
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Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases [PDF]
STAR ProtocolsSummary: Acyl carrier proteins (ACPs) are key domains in fatty acid and polyketide synthases (PKSs), shuttling intermediates to catalytic partners. Here, we present a protocol for purifying and analyzing ACPs from fatty acid synthases (FASs) and PKSs ...
Christian Gusenda +6 more
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Polyketides, Toxins and Pigments in Penicillium marneffei [PDF]
Toxins, 2015 Penicillium marneffei (synonym: Talaromyces marneffei) is the most important pathogenic thermally dimorphic fungus in China and Southeastern Asia. The HIV/AIDS pandemic, particularly in China and other Southeast Asian countries, has led to the emergence ...
Emily W. T. Tam +3 more
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ASMPKS: an analysis system for modular polyketide synthases [PDF]
BMC Bioinformatics, 2007 Background Polyketides are secondary metabolites of microorganisms with diverse biological activities, including pharmacological functions such as antibiotic, antitumor and agrochemical properties. Polyketides are synthesized by serialized reactions of a
Kong Eun-Bae, Tae Hongseok, Park Kiejung
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