Results 41 to 50 of about 23,262 (184)

Gating mechanism of elongating β-ketoacyl-ACP synthases

Nature Communications, 2020
The formation of C-C bonds in fatty acid and polyketide biosynthesis depends on β-ketoacyl-acyl carrier protein (ACP) synthases (KSs). Here, the authors present structures of E.coli KSs bound to substrate mimetic bearing ACPs, providing insights into the
Jeffrey T. Mindrebo, Ashay Patel, Woojoo E. Kim, Tony D. Davis, Aochiu Chen, Thomas G. Bartholow, James J. La Clair, J. Andrew McCammon, Joseph P. Noel, Michael D. Burkart   +9 more
doaj   +1 more source

Synthetic biology of fungal natural products [PDF]

, 2015
This work was supported by the DFG-funded excellence graduate school Jena School for Microbial Communication (JSMC; DJM, AAB), the collaborative research center (SFB) 1127 Chemical Mediators in Complex Biosystems (ChemBioSys—AAB, project B02) and the ...
Vito Valiante, Unkles, S.E., Mattern, D.J., Valiante, V., Brakhage, A.A., Derek J. Mattern, Axel A. Brakhage, Shiela E. Unkles   +7 more
core   +1 more source

Broad‐Spectrum, Cell Envelope‐Active Marinocyclin Antibiotics From a Coral‐Derived Bacterium Are Effective Against Colistin‐Resistant Bacteria

Angewandte Chemie, EarlyView.
Marinocyclins, polycationic macrocyclic depsipeptides, were discovered by genome mining from the marine coral‐derived bacterium Aquimarina megaterium. The compounds have broad‐spectrum activity against gram‐positive and gram‐negative ESKAPE bacteria.
Clara Chepkirui, Sandra Silva, Jan Straetener, Chandrashekhar Padhi, Tina Keller‐Costa, Tess Lamer, Laura Reinke, Heike Brötz‐Oesterhelt, Rodrigo Costa, Jörn Piel   +9 more
wiley   +2 more sources

Chemoinformatic-guided engineering of polyketide synthases [PDF]

Journal of the American Chemical Society, 2019
Abstract Polyketide synthase (PKS) engineering is an attractive method to generate new molecules such as commodity, fine and specialty chemicals. A significant challenge in PKS design is engineering a partially reductive module to produce a saturated β-carbon through a reductive loop exchange.
Amin Zargar, Ravi Lal, Luis Valencia, Jessica Wang, Tyler William H. Backman, Pablo Cruz-Morales, Ankita Kothari, Miranda Werts, Andrew R. Wong, Constance B. Bailey, Arthur Loubat, Yuzhong Liu, Yan Chen, Samantha Chang, Veronica T. Benites, Amanda C. Hernández, Jesus F. Barajas, Mitchell G. Thompson, Carolina Barcelos, Rasha Anayah, Hector Garcia Martin, Aindrila Mukhopadhyay, Christopher J. Petzold, Edward E. K. Baidoo, Leonard Katz, Jay D. Keasling   +25 more
openaire   +4 more sources

Manipulating polyketide stereochemistry by exchange of polyketide synthase modules

Chemical Communications, 2020
Exchange of polyketide synthase (PKS) modules by genetic engineering leads to efficient modification of polyketide stereochemistry.
Massicard, Jean-Malo, Soligot, Claire, Weissman, Kira J., Jacob, Christophe   +3 more
openaire   +4 more sources

Structure-activity relationship studies on the macrolide exotoxin mycolactone of Mycobacterium ulcerans [PDF]

, 2013
BACKGROUND: Mycolactones are a family of polyketide-derived macrolide exotoxins produced by Mycobacterium ulcerans, the causative agent of the chronic necrotizing skin disease Buruli ulcer.
Gerd Pluschke, Claudio Bomio, Philipp Gersbach, Scherr, Nicole, Jun Li, Claudio Bomio (396151), Nicole Scherr, Karl-Heinz Altmann, Jean-Pierre Dangy (42481), Gersbach, Philipp, Gersbach Philipp, Dangy Jean-Pierre, Jean-Pierre Dangy, Jun Li (6494), Pluschke Gerd, Li Jun, Bomio Claudio, Altmann, Karl-Heinz, Gerd Pluschke (7855), Philipp Gersbach (396150), Li, Jun, Scherr Nicole, Karl-Heinz Altmann (396152), Bomio, Claudio, Altmann Karl-Heinz, Pluschke, Gerd, Dangy, Jean-Pierre, Nicole Scherr (396149)   +27 more
core   +2 more sources

Emulating evolutionary processes to morph aureothin-type modular polyketide synthases and associated oxygenases

Nature Communications, 2019
The wealth of complex polyketides is an essential source for drug discovery. Here, the authors report an evolution-guided rational morphing of modular polyketide synthases (PKSs) for aurothin and neoaurothin biosynthesis, and reveal engineering site ...
Huiyun Peng, Keishi Ishida, Yuki Sugimoto, Holger Jenke-Kodama, Christian Hertweck   +4 more
doaj   +1 more source

Don't Classify Polyketide Synthases [PDF]

Chemistry & Biology, 2004
Polyketide synthases are intensively studied as metabolite factories generating diverse biologically active natural products. Contrary to their current classification as different "types," there is now a growing body of evidence illustrating that nature realized limitless transitional stages during evolution.
openaire   +2 more sources

Biocatalytic Synthesis of Stereospecific Triketide Lactones using Polyketide Synthases [PDF]

, 2016
Polyketide synthases are modular enzymes that create and modify large acyl chains. The domains and modules of polyketide synthases allow us to create molecules that resemble naturally occurring products by applying a biocatalytic in vitro in vivo ...
Alexander, Bradley
core   +1 more source

Probing Selectivity and Creating Structural Diversity Through Hybrid Polyketide Synthases [PDF]

, 2020
Engineering polyketide synthases (PKS) to produce new metabolites requires an understanding of catalytic points of failure during substrate processing. Growing evidence indicates the thioesterase (TE) domain as a significant bottleneck within engineered ...
Jennifer J. Schmidt, Andrew N. Lowell, Aaron A. Koch, Coburn, Katherine M., Koch, Aaron A., Sherman, David H., Katherine M. Coburn, Lowell, Andrew N., Chemler, Joseph A., Schmidt, Jennifer J., Hansen, Douglas A., Joseph A. Chemler, David H. Sherman, Douglas A. Hansen   +13 more
core   +1 more source