Results 31 to 40 of about 8,226 (181)

Suzuki–Miyaura Reaction in the Presence of N-Acetylcysteamine Thioesters Enables Rapid Synthesis of Biomimetic Polyketide Thioester Surrogates for Biosynthetic Studies

Chemistry, 2023
Biomimetic N-acetylcysteamine thioesters are essential for the study of polyketide synthases, non-ribosomal peptide synthetases and fatty acid synthases.
Sebastian Derra, Luca Schlotte, Frank Hahn   +2 more
doaj   +1 more source

Cleaning up Polyketide Synthases

Chemistry & Biology, 2012
Complex biosynthetic enzymes such as polyketide synthases make mistakes. In this issue of Chemistry & Biology, Jensen et al. report that a discrete family of acyltransferases is responsible for error correction, hydrolyzing key biosynthetic intermediates from a multi-enzyme complex. This activity might find use in understanding polyketide biosynthesis,
Kwan, Jason C., Schmidt, Eric W.
openaire   +2 more sources

A Wagner–Meerwein‐Like Rearrangement Generates a Vinyl Group in the Biosynthesis of the Polychlorinated Lipopeptides Fischerazoles

Angewandte Chemie, EarlyView.
The fischerazoles, unusual cyanobacterial chlorinated lipopeptides are reported. During their biosynthesis, a linear fatty acyl‐acyl carrier protein (ACP) precursor is rearranged through the action of the S‐adenosyl‐methionine (SAM)‐dependent methyltransferase FshF, to generate a pendant vinyl group.
Sandra A. C. Figueiredo, Kathleen Abt, Konrad Viehrig, Peter J. Jervis, Teresa P. Martins, Iñaki Lacomba, Chuhan Li, Daniele Castagnolo, Pedro N. Leão   +8 more
wiley   +2 more sources

New Kendomycin Derivative Isolated from Streptomyces sp. Cl 58-27

Molecules, 2021
In the course of screening new streptomycete strains, the strain Streptomyces sp. Cl 58-27 caught our attention due to its interesting secondary metabolite production profile.
Constanze Paulus, Oleksandr Gromyko, Andriy Luzhetskyy   +2 more
doaj   +1 more source

Gating mechanism of elongating β-ketoacyl-ACP synthases

Nature Communications, 2020
The formation of C-C bonds in fatty acid and polyketide biosynthesis depends on β-ketoacyl-acyl carrier protein (ACP) synthases (KSs). Here, the authors present structures of E.coli KSs bound to substrate mimetic bearing ACPs, providing insights into the
Jeffrey T. Mindrebo, Ashay Patel, Woojoo E. Kim, Tony D. Davis, Aochiu Chen, Thomas G. Bartholow, James J. La Clair, J. Andrew McCammon, Joseph P. Noel, Michael D. Burkart   +9 more
doaj   +1 more source

Chemoinformatic-guided engineering of polyketide synthases [PDF]

Journal of the American Chemical Society, 2019
Abstract Polyketide synthase (PKS) engineering is an attractive method to generate new molecules such as commodity, fine and specialty chemicals. A significant challenge in PKS design is engineering a partially reductive module to produce a saturated β-carbon through a reductive loop exchange.
Amin Zargar, Ravi Lal, Luis Valencia, Jessica Wang, Tyler William H. Backman, Pablo Cruz-Morales, Ankita Kothari, Miranda Werts, Andrew R. Wong, Constance B. Bailey, Arthur Loubat, Yuzhong Liu, Yan Chen, Samantha Chang, Veronica T. Benites, Amanda C. Hernández, Jesus F. Barajas, Mitchell G. Thompson, Carolina Barcelos, Rasha Anayah, Hector Garcia Martin, Aindrila Mukhopadhyay, Christopher J. Petzold, Edward E. K. Baidoo, Leonard Katz, Jay D. Keasling   +25 more
openaire   +4 more sources

Emulating evolutionary processes to morph aureothin-type modular polyketide synthases and associated oxygenases

Nature Communications, 2019
The wealth of complex polyketides is an essential source for drug discovery. Here, the authors report an evolution-guided rational morphing of modular polyketide synthases (PKSs) for aurothin and neoaurothin biosynthesis, and reveal engineering site ...
Huiyun Peng, Keishi Ishida, Yuki Sugimoto, Holger Jenke-Kodama, Christian Hertweck   +4 more
doaj   +1 more source

Manipulating polyketide stereochemistry by exchange of polyketide synthase modules

Chemical Communications, 2020
Exchange of polyketide synthase (PKS) modules by genetic engineering leads to efficient modification of polyketide stereochemistry.
Massicard, Jean-Malo, Soligot, Claire, Weissman, Kira J., Jacob, Christophe   +3 more
openaire   +4 more sources

Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase

Nature Communications, 2021
Engineering efforts have focused on acyltransferase (AT) domains of modular polyketide synthases (PKSs) to site-selectively modify the resulting polyketides, but critical AT residues involved in substrate selection have not been fully elucidated.
Edward Kalkreuter, Kyle S. Bingham, Aaron M. Keeler, Andrew N. Lowell, Jennifer J. Schmidt, David H. Sherman, Gavin J. Williams   +6 more
doaj   +1 more source

Evolutionary Histories of Type III Polyketide Synthases in Fungi

Frontiers in Microbiology, 2020
Type III polyketide synthases (PKSs) produce secondary metabolites with diverse biological activities, including antimicrobials. While they have been extensively studied in plants and bacteria, only a handful of type III PKSs from fungi has been ...
Jorge Carlos Navarro-Muñoz, Jérôme Collemare   +1 more
doaj   +1 more source