Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis [PDF]
Nature Communications, 2022 Cocaine is a tropane alkaloid produced by Erythroxylum novogranatense. Here the authors identify two polyketide synthases involved in cocaine biosynthesis and provide insight into the parallel evolution of these enzymes in tropane alkaloids-producing ...
Tian Tian +9 more
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Genomic mining and diversity of assembly line polyketide synthases [PDF]
Open Biology, 2023 Assembly line polyketide synthases (PKSs) are a large family of multifunctional enzymes responsible for synthesizing many medicinally relevant natural products with remarkable structural variety and biological activity.
Shreya Kishore, Chaitan Khosla
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Gene conversion-associated successive engineering of modular polyketide synthases [PDF]
Communications ChemistryModular polyketide synthases (PKSs) can produce various secondary metabolites in a collinearity fashion. Although rational engineering of modular PKS can ultimately create a diverse array of compounds, de novo generation of defined structures usually ...
Wenzheng Jin +4 more
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Basidiomycete non-reducing polyketide synthases function independently of SAT domains [PDF]
Fungal Biology and Biotechnology, 2023 Background Non-reducing polyketide synthases (NR-PKSs) account for a major share of natural product diversity produced by both Asco- and Basidiomycota. The present evolutionary diversification into eleven clades further underscores the relevance of these
Nikolai A. Löhr +5 more
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Diversity and Distribution of Non-Reducing Polyketide Synthases (NR-PKSs) in Ascomycota (Fungi) [PDF]
Journal of Fungi(1) Background: This study highlights the diversity and distribution of non-reducing polyketide synthases (NR-PKSs) in Ascomycota and their role in producing bioactive aromatic polyketides.
Pritam Chattopadhyay, Goutam Banerjee
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Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases [PDF]
STAR ProtocolsSummary: Acyl carrier proteins (ACPs) are key domains in fatty acid and polyketide synthases (PKSs), shuttling intermediates to catalytic partners. Here, we present a protocol for purifying and analyzing ACPs from fatty acid synthases (FASs) and PKSs ...
Christian Gusenda +6 more
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The Ketosynthase Domain Controls Chain Length in Mushroom Oligocyclic Polyketide Synthases [PDF]
ChemBioChem, 2023 Markus Gressler +2 more
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C–N bond formation by a polyketide synthase
Nature Communications, 2023 Assembly-line polyketide synthases (PKSs) are molecular factories that produce diverse metabolites with wide-ranging biological activities. PKSs usually work by constructing and modifying the polyketide backbone successively. Here, we present the cryo-EM
Jialiang Wang +14 more
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Methyltransferase Domain‐Focused Genome Mining for Fungal Polyketide Synthases [PDF]
Small MethodsYudai Matsuda, Dexiu Yan
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Nature Communications, 2020 Several different genetic strategies have been reported for the modification of polyketide synthases but the highly repetitive modular structure makes this difficult.
Kei Kudo +10 more
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