Results 1 to 10 of about 444 (104)

The Stereochemistry of Complex Polyketide Biosynthesis by Modular Polyketide Synthases

Molecules, 2011
Polyketides are a diverse class of medically important natural products whose biosynthesis is catalysed by polyketide synthases (PKSs), in a fashion highly analogous to fatty acid biosynthesis.
David Kwan, Frank Schulz
exaly   +3 more sources

C–N bond formation by a polyketide synthase

Nature Communications, 2023
Assembly-line polyketide synthases (PKSs) are molecular factories that produce diverse metabolites with wide-ranging biological activities. PKSs usually work by constructing and modifying the polyketide backbone successively. Here, we present the cryo-EM
Jialiang Wang, Xiaojie Wang, Xixi Li, LiangLiang Kong, Zeqian Du, Dandan Li, Lixia Gou, Hao Wu, Wei Cao, Xiaozheng Wang, Shuangjun Lin, Ting Shi, Zixin Deng, Zhijun Wang, Jingdan Liang   +14 more
doaj   +1 more source

Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis

Nature Communications, 2022
Cocaine is a tropane alkaloid produced by Erythroxylum novogranatense. Here the authors identify two polyketide synthases involved in cocaine biosynthesis and provide insight into the parallel evolution of these enzymes in tropane alkaloids-producing ...
Tian Tian, Yong-Jiang Wang, Jian-Ping Huang, Jie Li, Bingyan Xu, Yin Chen, Li Wang, Jing Yang, Yijun Yan, Sheng-Xiong Huang   +9 more
doaj   +1 more source

In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives

Nature Communications, 2020
Several different genetic strategies have been reported for the modification of polyketide synthases but the highly repetitive modular structure makes this difficult.
Kei Kudo, Takuya Hashimoto, Junko Hashimoto, Ikuko Kozone, Noritaka Kagaya, Reiko Ueoka, Takehiro Nishimura, Mamoru Komatsu, Hikaru Suenaga, Haruo Ikeda, Kazuo Shin-ya   +10 more
doaj   +1 more source

Engineering the stambomycin modular polyketide synthase yields 37-membered mini-stambomycins

Nature Communications, 2022
Genetic engineering of the type I polyketide synthases (PKSs) to produce desirable analogous remains largely inefficient. Here, the authors leverage multiple approaches to delete seven internal modules from the stambomycin PKS and generate 37-membered ...
Li Su, Laurence Hôtel, Cédric Paris, Clara Chepkirui, Alexander O. Brachmann, Jörn Piel, Christophe Jacob, Bertrand Aigle, Kira J. Weissman   +8 more
doaj   +1 more source

Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli

Metabolic Engineering Communications, 2020
Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical ...
Kai P. Yuet, Chaitan Khosla
doaj   +1 more source

Evolution of combinatorial diversity in trans-acyltransferase polyketide synthase assembly lines across bacteria

Nature Communications, 2021
Trans-acyltransferase polyketide synthases are multimodular enzymes that synthesise diverse polyketides. Here, the authors present an algorithm for the global study of their diversity, showing exchange of conserved consecutive modules as a driver of ...
Eric J. N. Helfrich, Reiko Ueoka, Marc G. Chevrette, Franziska Hemmerling, Xiaowen Lu, Stefan Leopold-Messer, Hannah A. Minas, Adrien Y. Burch, Steven E. Lindow, Jörn Piel, Marnix H. Medema   +10 more
doaj   +1 more source

Decrypting the programming of β-methylation in virginiamycin M biosynthesis

Nature Communications, 2023
Biosynthesis of complex polyketides by polyketide synthases often relies on trans-acting enzymes to modify the intermediates. Here, the authors elucidate how β-methylation enzymes identify their substrates.
Sabrina Collin, Russell J. Cox, Cédric Paris, Christophe Jacob, Benjamin Chagot, Kira J. Weissman, Arnaud Gruez   +6 more
doaj   +1 more source

Polymorphism of the Polyketide Synthase Gene phlD in Biocontrol Fluorescent Pseudomonads Producing 2,4-Diacetylphloroglucinol and Comparison of PhlD with Plant Polyketide Synthases

Molecular Plant-Microbe Interactions, 2001
Many biocontrol fluorescent pseudomonads can protect plants from soilborne fungal pathogens through production of the antifungal secondary metabolite 2,4-diacetylphloroglucinol (Phl). One of the phl biosynthetic genes, phlD, encodes a polyketide synthase
Alban Ramette, Yvan Moënne-Loccoz, Geneviève Défago   +2 more
doaj   +1 more source

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis

BMC Biology, 2022
Background Type I polyketide synthases (PKSs) are multifunctional enzymes responsible for the biosynthesis of a group of diverse natural compounds with biotechnological and pharmaceutical interest called polyketides.
Cécile Bon, Stéphanie Cabantous, Sylviane Julien, Valérie Guillet, Christian Chalut, Julie Rima, Yoann Brison, Wladimir Malaga, Angelique Sanchez-Dafun, Sabine Gavalda, Annaïk Quémard, Julien Marcoux, Geoffrey S. Waldo, Christophe Guilhot, Lionel Mourey   +14 more
doaj   +1 more source