Results 31 to 40 of about 15,660 (211)

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis

BMC Biology, 2022
Background Type I polyketide synthases (PKSs) are multifunctional enzymes responsible for the biosynthesis of a group of diverse natural compounds with biotechnological and pharmaceutical interest called polyketides.
Cécile Bon, Stéphanie Cabantous, Sylviane Julien, Valérie Guillet, Christian Chalut, Julie Rima, Yoann Brison, Wladimir Malaga, Angelique Sanchez-Dafun, Sabine Gavalda, Annaïk Quémard, Julien Marcoux, Geoffrey S. Waldo, Christophe Guilhot, Lionel Mourey   +14 more
doaj   +1 more source

The role of the phosphopantetheinyltransferase enzyme, PswP, in the biosynthesis of antimicrobial secondary metabolites by Serratia marcescens Db10 [PDF]

, 2014
Phosphopantetheinyltransferase (PPTase) enzymes fulfil essential roles in primary and secondary metabolism in prokaryotes, archaea and eukaryotes. PPTase enzymes catalyse the essential modification of the carrier protein domain of fatty acid synthases ...
Amy J. Gerc, Elovson, Flyg, Herrero, Matsuyama, Nicola R. Stanley-Wall, Perego, Persmark, Sarah J. Coulthurst, Seyedsayamdost   +9 more
core   +4 more sources

Module-Based Polyketide Synthase Engineering for de Novo Polyketide Biosynthesis [PDF]

ACS Synthetic Biology, 2023
Polyketide retrobiosynthesis, where the biosynthetic pathway of a given polyketide can be reversibly engineered due to the colinearity of the polyketide synthase (PKS) structure and function, has the potential to produce millions of organic molecules. Mixing and matching modules from natural PKSs is one of the routes to produce many of these molecules.
Alberto A. Nava, Jacob Roberts, Robert W. Haushalter, Zilong Wang, Jay D. Keasling   +4 more
openaire   +4 more sources

Expanding the Utility of Bioinformatic Data for the Full Stereostructural Assignments of Marinolides A and B, 24- and 26-Membered Macrolactones Produced by a Chemically Exceptional Marine-Derived Bacterium

Marine Drugs, 2023
Marinolides A and B, two new 24- and 26-membered bacterial macrolactones, were isolated from the marine-derived actinobacterium AJS-327 and their stereostructures initially assigned by bioinformatic data analysis.
Min Cheol Kim, Jaclyn M. Winter, Reiko Cullum, Alexander J. Smith, William Fenical   +4 more
doaj   +1 more source

Mining microbial genomes for new natural products and biosynthetic pathways [PDF]

, 2008
Analyses of microbial genome sequences have revealed numerous examples of ‘cryptic’ or ‘orphan’ biosynthetic gene clusters, with the potential to direct the production of novel, structurally complex natural products.
Austin, Austin, Banskota, Banskota, Barona-Gómez, Barona-Gómez, Bentley, Bergmann, Bok, Caffrey, Chain, Challis, Challis, Challis, Challis, Chen, Chen, Corre, de Bruijn, Drake, Erb, Fischbach, Fleischmann, Fleming, Funa, Gregory L. Challis, Gross, Gross, Grüschow, Gust, Günter, Haydock, Haynes, Hojati, Hornung, Ikeda, Izumikawa, Kadi, Keller, Koehn, Larsen, Lautru, Lautru, Li, Li, Lin, Lin, McAlpine, Miethke, Minowa, Miyanaga, Muller, Nguyen, Oliynyk, Omura, Paulsen, Petersen, Pfeifer, Rausch, Reid, Song, Stachelhaus, Sudek, Tohyama, Udwary, Wilkinson, Zhao, Zirkle   +67 more
core   +2 more sources

A single amino acid residue controls acyltransferase activity in a polyketide synthase from Toxoplasma gondii

iScience, 2022
Summary: Type I polyketide synthases (PKSs) are multidomain, multimodule enzymes capable of producing complex polyketide metabolites. These modules contain an acyltransferase (AT) domain, which selects acyl-CoA substrates to be incorporated into the ...
Hannah K. D’Ambrosio, Jack G. Ganley, Aaron M. Keeler, Emily R. Derbyshire   +3 more
doaj   +1 more source

Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening [PDF]

, 2003
New secondary mesenchyme specific genes, expressed exclusively in pigment cells, were isolated from sea urchin embryos using a differential screening of a macroarray cDNA library. The comparison was performed between mRNA populations of embryos having an
Calestani, Cristina, Davidson, Eric H., Rast, Jonathan P.   +2 more
core   +1 more source

Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens.

PLoS ONE, 2018
Usnic acid is a unique polyketide produced by lichens. To characterize usnic acid biosynthesis, the transcriptome of the usnic-acid-producing lichen-forming fungus Nephromopsis pallescens was sequenced using Illumina NextSeq technology.
Yi Wang, Changan Geng, Xiaolong Yuan, Mei Hua, Fenghua Tian, Changtian Li   +5 more
doaj   +1 more source

Expansion and Conservation of Biosynthetic Gene Clusters in Pathogenic Pyrenophora spp.

Toxins, 2020
Pyrenophora is a fungal genus responsible for a number of major cereal diseases. Although fungi produce many specialised or secondary metabolites for defence and interacting with the surrounding environment, the repertoire of specialised metabolites (SM)
Paula M. Moolhuijzen, Mariano Jordi Muria-Gonzalez, Robert Syme, Catherine Rawlinson, Pao Theen See, Caroline S. Moffat, Simon R. Ellwood   +6 more
doaj   +1 more source

Suzuki–Miyaura Reaction in the Presence of N-Acetylcysteamine Thioesters Enables Rapid Synthesis of Biomimetic Polyketide Thioester Surrogates for Biosynthetic Studies

Chemistry, 2023
Biomimetic N-acetylcysteamine thioesters are essential for the study of polyketide synthases, non-ribosomal peptide synthetases and fatty acid synthases.
Sebastian Derra, Luca Schlotte, Frank Hahn   +2 more
doaj   +1 more source