Results 81 to 90 of about 8,226 (181)

Deciphering the Function and Structure of PA1216 as an S‐Adenosyl‐l‐Methionine Binding Protein Using Differential Scanning Fluorimetry and Circular Dichroism

Protein Science, Volume 35, Issue 8, August 2026.
Abstract Microbes produce bioactive secondary metabolites as toxins, pigments, or virulence factors. These specialized compounds are produced by nonribosomal peptide synthetases (NRPS), polyketide synthases (PKS), or hybrid NRPS/PKS pathways. The genes encoding NRPS and PKS reside in biosynthetic gene clusters (BGCs), some of which have no identified ...
Amaan M. Fruitwala, Jade X. Tiszler, Daniel Watson, Mansi Sinha, Tony D. Davis   +4 more
wiley   +1 more source

Structure and function of the geldanamycin amide synthase from Streptomyces hygroscopicus

Nature Communications
Amide synthases catalyze the formation of macrolactam rings from aniline-containing polyketide-derived seco-acids as found in the important class of ansamycin antibiotics.
Wiebke Ewert, Christian Bartens, Jekaterina Ongouta, Monika Holmes, Anja Heutling, Anusha Kishore, Tim Urbansky, Carsten Zeilinger, Matthias Preller, Andreas Kirschning   +9 more
doaj   +1 more source

Barley HvBODYGUARD1 controls cuticular specialisations regulated by SHINE transcription factors

New Phytologist, Volume 251, Issue 3, Page 1188-1208, August 2026.
Cuticle defects result from defective HvBDG1 alleles. Summary Land plants secrete a protective outer cuticular layer with diverse functions. Barley (Hordeum vulgare L.) develops two cuticular specialisations: the β‐diketone rich wax bloom on vegetative tissues and an adherent grain surface which sticks to the hulls, leading to barley's distinctive ...
Trisha McAllister, Chiara Campoli, Linsan Liu, Tansy Chia, S. Ronan Fisher, Richard Horsnell, Alan R. Prescott, Jennifer Shoesmith, Mhmoud Eskan, Alasdair Iredale, Mirjam Nuter, Luke Ramsay, Micha M. Bayer, Linda Milne, Miriam Schreiber, Yogeswari Rajarathinam, Vanessa Wahl, Robbie Waugh, James Cockram, Sarah M. McKim   +19 more
wiley   +1 more source

A Polyketide Synthase Gene, ACRTS2, Is Responsible for Biosynthesis of Host-Selective ACR-Toxin in the Rough Lemon Pathotype of Alternaria alternata

Molecular Plant-Microbe Interactions, 2012
The rough lemon pathotype of Alternaria alternata produces host-selective ACR-toxin and causes Alternaria leaf spot disease of rough lemon (Citrus jambhiri). The structure of ACR-toxin I (MW = 496) consists of a polyketide with an α-dihydropyrone ring in
Y. Izumi, K. Ohtani, Y. Miyamoto, A. Masunaka, T. Fukumoto, K. Gomi, Y. Tada, K. Ichimura, T. L. Peever, K. Akimitsu   +9 more
doaj   +1 more source

First Tetraploa Genome and Multi‐Omics Analysis Reveal Key Plant‐Microbe‐Soil Interactions for Salt Tolerance and Yield Improvement of Wheat

Plant Biotechnology Journal, Volume 24, Issue 8, Page 4748-4765, August 2026.
ABSTRACT Salinity is a major threat to global agricultural productivity of staple crops such as wheat. Although microbial‐based solutions hold promise for alleviating salinity stress, practical implementation is hindered by insufficient mechanistic characterization of bioinoculants and their interactions with plants.
Cheng‐Wei Qiu, Shuo Zhang, Zi‐Feng Gao, Zhong‐Hua Chen, Chulong Zhang, Mohamed Abdelalim Ali, Feibo Wu   +6 more
wiley   +1 more source

Strategies in megasynthase engineering – fatty acid synthases (FAS) as model proteins

Beilstein Journal of Organic Chemistry, 2017
Megasynthases are large multienzyme proteins that produce a plethora of important natural compounds by catalyzing the successive condensation and modification of precursor units.
Manuel Fischer, Martin Grininger
doaj   +1 more source

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues

ChemBioChem, Volume 27, Issue 13, 14 July 2026.
A mutasynthetic strategy was developed to prepare pseudomonic acid analogues, and their antimicrobial activities were evaluated against five bacterial strains. Feeding studies with mutant strains of Pseudomonas fluorescens revealed that C7‐desepoxy‐PA‐B was metabolised to C7‐PA‐C with loss of the 8‐hydroxyl group, but no fatty acid chain extension ...
Sarah M. Husain, Luoyi Wang, Li‐Chen Han, James I. Bowen, Zhongshu Song, Felix de Courcy‐Ireland, Ashley J. Winter, Thomas J. Simpson, Paul R. Race, James Spencer, Matthew P. Crump, Christine L. Willis   +11 more
wiley   +1 more source

PKMiner: a database for exploring type II polyketide synthases

BMC Microbiology, 2012
Background Bacterial aromatic polyketides are a pharmacologically important group of natural products synthesized by type II polyketide synthases (type II PKSs) in actinobacteria.
Kim Jinki, Yi Gwan-Su
doaj   +1 more source

Dissection and Engineering of Modular Polyketide Synthase Extender Unit Specificity Motifs

ChemBioChem, Volume 27, Issue 13, 14 July 2026.
Motif‐level engineering of acyltransferase domains in polyketide synthases enables programable extender unit selectivity. Cooperative mutations and motif exchanges reprogram substrate preference, enabling the incorporation of non‐native extender units and expanding polyketide structural diversity, with gains in activity and product yield.
Sydney Welch, Gavin J. Williams
wiley   +1 more source

Molecular basis for depsipeptide HDAC inhibitor combinatorial biosynthesis

Nature Communications
Polyketides and nonribosomal peptides are important natural product classes with wide-ranging medical and agricultural applications. The analogous enzymatic logic employed by bacterial modular polyketide synthases (PKSs) and nonribosomal peptide ...
Munro Passmore, Xinyun Jian, Xinyi Zhao, Emmanuel L. C. de los Santos, Douglas M. Roberts, Józef R. Lewandowski, Matthew Jenner, Lona M. Alkhalaf, Gregory L. Challis   +8 more
doaj   +1 more source