Results 11 to 20 of about 1,993,919 (353)

Microfluidic polymerase chain reaction [PDF]

, 2008
We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of ...
Gomez, Alvaro, Gomez, Frank A., Maltezos, George, Scherer, Axel, Zhong, Jiang   +4 more
core   +1 more source

Effectiveness of rotavirus vaccination in prevention of hospital admissions for rotavirus gastroenteritis among young children in Belgium : case-control study [PDF]

, 2012
Objective : To evaluate the effectiveness of rotavirus vaccination among young children in Belgium. Design : Prospective case-control study. Setting : Random sample of 39 Belgian hospitals, February 2008 to June 2010.
A. Vergison, A.-S. Maernoudt, E. Heylen, H. Capiau, J. De Koster, J. Matthijnssens, J.-Y. Pircon, K. Van Herck, L. Verdonck, M. Azou, M. Raes, M. Soriano-Gabarro, M. Van Ranst, M. Verghote, M. Zeller, N. Meyer, null null, P. Van Damme, T. Braeckman   +18 more
core   +1 more source

Diagnostic Performance of CT and Reverse Transcriptase-Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis

Radiology, 2020
Background Recent studies have suggested that chest computed tomography (CT) scans could be used as a primary screening or diagnostic tool for coronavirus disease 2019 (COVID-19) in epidemic areas.
Hyungjin Kim, Hyunsook Hong, S. Yoon
semanticscholar   +1 more source

Polymerase chain displacement reaction

BioTechniques, 2013
Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris, Irma J. Sanchez-Vargas, Ken E. Olson, Luke Alphey, Guoliang Fu   +4 more
doaj   +1 more source

The polymerase chain reaction [PDF]

, 2003
The Polymerase Chain Reaction (PCR) is a technique for the in vitro synthesis of billions of copies of a specific nucleic acid sequence by performing successive rounds of in vitro nucleic acid replication.
Paulton, M P, Thomas, P C
core   +1 more source

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

Veterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal, Sunit Kumar Mukhopadhayay, Saktipada Pradhan, Samiran Mondal, Chandrakanta Jana, Nimai Chandra Patra, Rabindra Nath Hansda   +6 more
doaj   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

Frontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin, Pengzhen Jin, Xiaoyang Gao, Xiaoyang Gao, Miaomiao Wang, Miaomiao Wang, Yeqing Qian, Yeqing Qian, Jingjin Yang, Jingjin Yang, Yanmei Yang, Yanmei Yang, Yuqing Xu, Yuqing Xu, Yanfei Xu, Yanfei Xu, Minyue Dong, Minyue Dong   +17 more
doaj   +1 more source

P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer. [PDF]

, 1996
Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens ...
Chen, KM, Chui, RM, Dahiya, R, Deng, G, Haughney, PC, Narayan, P   +5 more
core   +2 more sources

False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Korean Journal of Radiology, 2020
The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide.
Dasheng Li, Dawei Wang, Jianping Dong, Nan Wang, He Huang, Haiwang Xu, Chen Xia   +6 more
semanticscholar   +1 more source

Immunological diagnosis as an adjunctive tool for an early diagnosis of tuberculous meningitis of an immune competent child in a low tuberculosis endemic country: A case report [PDF]

, 2017
Background: Pediatric tuberculous meningitis is a highly morbid, often fatal disease. Its prompt diagnosis and treat - ment saves lives, in fact delays in the initiation of therapy have been associated with high mortality rates. Case presentation:
Ajassa, Camilla, Caraffa, Emanuela, Ciardi, Rosa Maria, Colistra, Davide, DI BIASI, Claudio, Lichtner, Miriam, Mancarella, Cristina, Mascia, Claudia, Mastroianni, Claudio Maria, Mengoni, Fabio, Paglia, Maria Grazia, Vita, Serena, Vullo, Vincenzo   +12 more
core   +4 more sources