Relevance of micrometastases detected by reverse transcriptase-polymerase chain reaction for melanoma recurrence: systematic review and meta-analysis [PDF]
São Paulo Medical Journal, 2003 CONTEXT: Cutaneous melanoma presents significant morbidity and mortality. Nowadays, about 90% of them are diagnosed by clinical examination and most are localized melanomas.
Allisson Monteiro da Silva +3 more
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Microfluidic polymerase chain reaction [PDF]
, 2008 We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of ...
Gomez, Alvaro +4 more
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The polymerase chain reaction [PDF]
, 2003 The Polymerase Chain Reaction (PCR) is a technique for the in vitro synthesis of billions of copies of a specific nucleic acid sequence by performing successive rounds of in vitro nucleic acid replication.
Paulton, M P, Thomas, P C
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Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]
Veterinary World, 2017 Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal +6 more
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P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer. [PDF]
, 1996 Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens ...
Chen, KM +5 more
core +2 more sources
Frontiers in Genetics, 2021 Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin +17 more
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Effectiveness of rotavirus vaccination in prevention of hospital admissions for rotavirus gastroenteritis among young children in Belgium : case-control study [PDF]
, 2012 Objective : To evaluate the effectiveness of rotavirus vaccination among young children in Belgium. Design : Prospective case-control study. Setting : Random sample of 39 Belgian hospitals, February 2008 to June 2010.
A. Vergison +18 more
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On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]
, 2007 We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
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Polymerase Chain Reaction [PDF]
SSRN Electronic Journal, 2020 The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a ...
Aakanchha Jain, Richa Jain, Sourabh Jain
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DNA extraction and polymerase chain reaction
Journal of Cytology, 2019 DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
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