Results 11 to 20 of about 1,395,900 (346)

Identification of group A rotavirus gene 4 types by polymerase chain reaction [PDF]

open access: bronzeJournal of Clinical Microbiology, 1992
Jon R. Gentsch   +7 more
openalex   +2 more sources

Spoligotyping and polymerase chain reaction based mycobacterium bovis strains typing with methods (enterobacterial repetitive intergenic consensus-polymerase chain reaction, randomly amplified polymorphic dnas-polymerase chain reaction and out polymerase chain reaction)

open access: yesInternational Journal of Mycobacteriology, 2022
Background: In this study, it was aimed to investigate Mycobacterium bovis strains isolated from lungs and lymph nodes of slaughtered animals on clonal level by using different methods such as spoligotyping, enterobacterial repetitive intergenic ...
Funda Sahin   +4 more
doaj   +1 more source

Polymerase Chain Reaction [PDF]

open access: yesJournal of Investigative Dermatology, 2013
The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, 1990). For the first time, it allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project.
Nidhi Avashia, Lilit Garibyan
openaire   +2 more sources

Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction–Based SARS-CoV-2 Tests by Time Since Exposure

open access: yesAnnals of Internal Medicine, 2020
Background: Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on reverse transcriptase polymerase chain reaction (RT-PCR) are being used to “rule out” infection among high-risk persons, such as exposed inpatients and health ...
L. Kucirka   +4 more
semanticscholar   +1 more source

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

open access: yesBMC Bioinformatics, 2012
Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and ...
Jian Ye   +5 more
semanticscholar   +1 more source

Polymerase chain reaction [PDF]

open access: yesBMJ, 1997
The polymerase chain reaction (PCR) was devised just over a decade ago, yet it is already an integral part of much biological and medical research. A glance at current journal articles shows that it is also being used to develop new diagnostic tests, which are already having an impact on clinical practice.
openaire   +3 more sources

Polymerase Chain Reaction [PDF]

open access: yesCold Spring Harbor Protocols, 2019
The polymerase chain reaction (PCR) underlies almost all of modern molecular cloning. Using PCR, a defined target sequence that occurs once within a DNA of high complexity and large size—an entire mammalian genome, for example—can be rapidly and selectively amplified in a quasi-exponential chain reaction that generates millions of copies.
Michael R. Green, Joseph Sambrook
openaire   +3 more sources

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

open access: yesVeterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal   +6 more
doaj   +1 more source

Diagnostic Performance of CT and Reverse Transcriptase-Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis

open access: yesRadiology, 2020
Background Recent studies have suggested that chest computed tomography (CT) scans could be used as a primary screening or diagnostic tool for coronavirus disease 2019 (COVID-19) in epidemic areas.
Hyungjin Kim, Hyunsook Hong, S. Yoon
semanticscholar   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

open access: yesFrontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin   +17 more
doaj   +1 more source

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