Results 11 to 20 of about 1,450,683 (312)

A portable low-cost polymerase chain reaction device [PDF]

HardwareX
Polymerase chain reaction (PCR) is a cornerstone technique in molecular biology and clinical diagnostics. However, conventional PCR systems are often bulky and prohibitively expensive, limiting their use in resource-limited settings.
Kan Luo, Wei Cheng, Yu Chen, Qirong Zhang, Chaobing Liang, Jianxing Li, Wu Wang   +6 more
doaj   +2 more sources

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

BMC Bioinformatics, 2012
Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and ...
Jian Ye, G. Coulouris, I. Zaretskaya, I. Cutcutache, S. Rozen, Thomas L. Madden   +5 more
semanticscholar   +1 more source

Polymerase chain displacement reaction

BioTechniques, 2013
Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris, Irma J. Sanchez-Vargas, Ken E. Olson, Luke Alphey, Guoliang Fu   +4 more
doaj   +1 more source

Diagnostic Performance of CT and Reverse Transcriptase-Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis

Radiology, 2020
Background Recent studies have suggested that chest computed tomography (CT) scans could be used as a primary screening or diagnostic tool for coronavirus disease 2019 (COVID-19) in epidemic areas.
Hyungjin Kim, Hyunsook Hong, S. Yoon
semanticscholar   +1 more source

False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Korean Journal of Radiology, 2020
The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide.
Dasheng Li, Dawei Wang, Jianping Dong, Nan Wang, He Huang, Haiwang Xu, Chen Xia   +6 more
semanticscholar   +1 more source

Computational Investigations on Polymerase Actions in Gene Transcription and Replication Combining Physical Modeling and Atomistic Simulations [PDF]

Chin. Phys. B Vol. 25, No. 1 (2016) 018706, 2015
Polymerases are protein enzymes that move along nucleic acid chains and catalyze template-based polymerization reactions during gene transcription and replication. The polymerases also substantially improve transcription or replication fidelity through the non-equilibrium enzymatic cycles.
arxiv   +1 more source

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

Journal of Molecular Endocrinology, 2000
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples.
S. Bustin
semanticscholar   +1 more source

Confidence intervals for nonhomogeneous branching processes and polymerase chain reactions [PDF]

Annals of Probability 2005, Vol. 33, No. 2, 674-702, 2005
We extend in two directions our previous results about the sampling and the empirical measures of immortal branching Markov processes. Direct applications to molecular biology are rigorous estimates of the mutation rates of polymerase chain reactions from uniform samples of the population after the reaction. First, we consider nonhomogeneous processes,
arxiv   +1 more source

Contribution of neutrophil/lymphocyte ratio to the diagnostic efficiency of computed tomography and polymerase chain reaction in COVID-19 patients

SAGE Open Medicine, 2021
Background: 6.5% of the country’s population was diagnosed with COVID-19 disease. Computed tomography scanning and polymerase chain reaction tests are considered reliable methods for the detection of COVID-19.
Serhat Örün, Mustafa Numan Erdem
doaj   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

Frontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin, Pengzhen Jin, Xiaoyang Gao, Xiaoyang Gao, Miaomiao Wang, Miaomiao Wang, Yeqing Qian, Yeqing Qian, Jingjin Yang, Jingjin Yang, Yanmei Yang, Yanmei Yang, Yuqing Xu, Yuqing Xu, Yanfei Xu, Yanfei Xu, Minyue Dong, Minyue Dong   +17 more
doaj   +1 more source