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Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control ...
Tuti Susanti Legiastuti, Tri Aminingsih
doaj +1 more source
Detection of Brucella canis in blood, urine and seminal fluid of a naturally infected dog by PCR
A brucelose canina é uma doença bacteriana, infectocontagiosa que acomete cães de todo o mundo.O diagnóstico laboratorial é baseado em métodos diretos e indiretos e deve ser realizado quando há evidências de sinais clínicos e epidemiológicos.
Fabiana Sperb Volkweis+5 more
doaj +1 more source
Semi-quantitative digital analysis of polymerase chain reactionelectrophoresis gel: Potential applications in low-income veterinary laboratories [PDF]
Aim: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a ...
John F. Antiabong+2 more
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The polymerase chain reaction (PCR) underlies almost all of modern molecular cloning. Using PCR, a defined target sequence that occurs once within a DNA of high complexity and large size-an entire mammalian genome, for example-can be rapidly and ...
Michael R Green, J. Sambrook
semanticscholar +2 more sources
Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt [PDF]
Aim: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella ...
Hazem Mohammed Ibrahim+3 more
doaj +1 more source
Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.
Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro.
R. Horton, Z. Cai, S. Ho, L. Pease
semanticscholar +1 more source
Background Determining the infectiousness of patients with coronavirus disease 2019 is crucial for patient management. Medical staff usually refer to the results of reverse transcription polymerase chain reaction tests in conjunction with clinical ...
Masafumi Kanamoto+3 more
doaj +1 more source
Eradication of Helicobacter pylori Infection Using 7-day PCR-based Tailored Therapy [PDF]
Background/Aims Standard triple therapy (STT; proton pump inhibitor [PPI]+clarithromycin+amoxicillin) used for Helicobacter pylori (H. pylori) eradication has shown low treatment success rates in recent years, which is most likely attributable to ...
Youngwoo Kim+6 more
doaj +1 more source
Nanozyme-based biosensing for clinical diagnosis of COVID-19: A mini review [PDF]
Several clinical methods had been utilized for diagnosis of COVID-19 for instance, real-time reverse transcription-polymerase chain reaction (rRT-PCR), hematology examination, polymerase chain reaction (PCR), diagnostic guidelines based on clinical features, and Chest CT scans.
arxiv
Rickettsia prowazekii and Real-time Polymerase Chain Reaction
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka+4 more
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