Results 21 to 30 of about 1,398,764 (327)

DNA extraction and polymerase chain reaction

Journal of Cytology, 2019
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
doaj   +1 more source

Contribution of neutrophil/lymphocyte ratio to the diagnostic efficiency of computed tomography and polymerase chain reaction in COVID-19 patients

SAGE Open Medicine, 2021
Background: 6.5% of the country’s population was diagnosed with COVID-19 disease. Computed tomography scanning and polymerase chain reaction tests are considered reliable methods for the detection of COVID-19.
Serhat Örün, Mustafa Numan Erdem
doaj   +1 more source

Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction (Detection of Endophytic Fungi Using Polymerase Chain Reaction Technique)

Jurnal Fitopatologi Indonesia, 2013
Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control ...
Tuti Susanti Legiastuti, Tri Aminingsih
doaj   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

Frontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin, Pengzhen Jin, Xiaoyang Gao, Xiaoyang Gao, Miaomiao Wang, Miaomiao Wang, Yeqing Qian, Yeqing Qian, Jingjin Yang, Jingjin Yang, Yanmei Yang, Yanmei Yang, Yuqing Xu, Yuqing Xu, Yanfei Xu, Yanfei Xu, Minyue Dong, Minyue Dong   +17 more
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On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Detection of Brucella canis in blood, urine and seminal fluid of a naturally infected dog by PCR

Brazilian Journal of Veterinary Medicine, 2018
A brucelose canina é uma doença bacteriana, infectocontagiosa que acomete cães de todo o mundo.O diagnóstico laboratorial é baseado em métodos diretos e indiretos e deve ser realizado quando há evidências de sinais clínicos e epidemiológicos.
Fabiana Sperb Volkweis, Lilian Cristina Cavalcanti, Hélio Blume, Helvécio Leal Santos Júnior, Andrea Lazzari, Fernanda Mulinari   +5 more
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Semi-quantitative digital analysis of polymerase chain reactionelectrophoresis gel: Potential applications in low-income veterinary laboratories [PDF]

Veterinary World, 2016
Aim: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a ...
John F. Antiabong, Mafora G. Ngoepe, Adakole S. Abechi   +2 more
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Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt [PDF]

Veterinary World, 2016
Aim: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella ...
Hazem Mohammed Ibrahim, Dalia Ahmed Mohammed Abd El-moaty, Hanan Ali Ahmed, Mona Ibrahim El-Enbaawy   +3 more
doaj   +1 more source

Eradication of Helicobacter pylori Infection Using 7-day PCR-based Tailored Therapy [PDF]

The Korean Journal of Helicobacter and Upper Gastrointestinal Research, 2023
Background/Aims Standard triple therapy (STT; proton pump inhibitor [PPI]+clarithromycin+amoxicillin) used for Helicobacter pylori (H. pylori) eradication has shown low treatment success rates in recent years, which is most likely attributable to ...
Youngwoo Kim, Heejun Kang, Sang-Gon Moon, Bohyoung Kim, Hyeyeon Lee, Chul-Hyun Lim, Jung-Hwan Oh   +6 more
doaj   +1 more source

COVID-19 with repeated positive test results for SARS-CoV-2 by PCR and then negative test results twice during intensive care: a case report

Journal of Medical Case Reports, 2020
Background Determining the infectiousness of patients with coronavirus disease 2019 is crucial for patient management. Medical staff usually refer to the results of reverse transcription polymerase chain reaction tests in conjunction with clinical ...
Masafumi Kanamoto, Masaru Tobe, Tomonori Takazawa, Shigeru Saito   +3 more
doaj   +1 more source