Results 161 to 170 of about 4,583 (217)

Multiplex-terminal restriction fragment length polymorphism

Nature Protocols, 2006
A novel method called "multiplex-terminal restriction fragment length polymorphism (M-TRFLP)" has been recently developed which can be used for simultaneous analysis of the community composition of two or more microbial taxa (up to four). This method can also be used for microbial diagnostic purposes. For M-TRFLP analysis, primers specific to different
Singh, Brajesh K. (R15253), Thomas, Nadine   +1 more
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Restriction Fragment Length Polymorphism Analysis

Current Protocols in Human Genetics, 1994
AbstractThis unit describes Restriction Fragment Length Polymorphism (RFLP) analysis, which utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA regions of interest. When investigating families for inheritance of an RFLP, aliquots of genomic DNA from individual family members are digested to completion with
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Definition of DRw10 by restriction fragment length polymorphism

Tissue Antigens, 1989
To better define the presence of the DRw10 haplotype which has sometimes proved difficult to type by using serologic reagents, Southern blot analysis was performed on seven DRw10 heterozygous individuals with rheumatoid arthritis. Using the restriction enzymes Taq I or BamH I, the restriction fragment length polymorphism (RFLP) pattern for the DRw10 ...
S, Lee, A, Nuñez-Roldan, E, Dwyer, L, Pompeo, R, Winchester   +4 more
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Restriction fragment length polymorphism diversity in soybean

Theoretical And Applied Genetics, 1989
Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers
P, Keim, R C, Shoemaker, R G, Palmer
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Restriction fragment length polymorphisms of Anisakinae larvae

Journal of Helminthology, 1989
ABSTRACTThe analysis of restriction fragment length polymorphisms (RFLPs) was applied to distinguish several kinds of Anisakinae larvae, Anisakis larvae (type I) collected from two different paratenic hosts, Anisakis larvae (type II) and Contracaecum larvae. The patterns of the two different paratenic host-derived DNA of Anisakis larva (I) were exactly
K, Sugane, L, Qing, T, Matsuura
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Restriction Fragment Length Polymorphism of the D1S1 Locus in Italy

Human Heredity, 1988
Three Italian populations were examined for a restriction fragment length polymorphism probing with a DNA sequence of unknown function located on chromosome 1. No difference was observed between the samples. The allele frequencies in Italy were: D1S1 BS = 0.82, D1S1 BF = 0.18.
Palmarino R, Mantuano E, Lucarelli P
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Restriction fragment length polymorphism

1988
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA.
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Length polymorphisms of restriction fragments of rat mitochondrial DNAs

Biochemical and Biophysical Research Communications, 1981
Abstract Differences were found in the HpaII cleavage patterns of two types of rat ( Rattus norvegicus ) mtDNA, types A and B. One HpaII fragment, Hpa5, of type A was about 30 base pairs smaller than that of type B, but no 30-base pair fragment was detectable in an HpaII digest of type A mtDNA.
J I, Hayashi, O, Gotoh, Y, Tagashira
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Linkage of restriction fragment length polymorphisms and isozymes in Citrus

Theoretical and Applied Genetics, 1992
Genetic linkage analysis was performed using two segregating populations of citrus. One population arose from an intergeneric backcross of Citrus grandis (L.) Osb. cv 'Thong Dee' and Poncirus trifoliata (L.) Raf. cv 'Pomeroy', using the former as the recurrent (female) parent. The other population came from an interspecific backcross of C.
R E, Durham, P C, Liou, F G, Gmitter, G A, Moore   +3 more
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The Application of Restriction Fragment Length Polymorphism to Plant Breeding

1983
With the advent of the use of restriction endonucleases to map gene sequences, molecular geneticists have encountered widespread polymorphism for the location of restriction sites among individuals of a species. This observation is not surprising considering the number of supposedly neutral mutations encountered at the protean level in recent years ...
Burr, B., Evola, S. V., Burr, F. A., Beckmann, J. S.   +3 more
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