Strategies and mechanisms of precision genome engineering: From gene editing to genome writing
In this review, we examined the progression of genome manipulation from stochastic nuclease‐mediated cutting toward precise editing and programmable genome writing. We discussed tools like multi‐kilobase RNA‐guided integrators and Artificial Intelligence (AI)‐designed effectors and showed how these advances enable researchers to treat genomes as ...
Kerui Huang +19 more
wiley +1 more source
We present two near‐complete triticale genome assemblies and perform pan‐centromere analyses across >200 haplotypes from parental species, synthetic allopolyploids, cultivars, and 337 resequenced accessions, revealing a shared chromatin framework underlying functional centromeres in all three subgenomes, where wheat‐ and rye‐derived CENH3 co‐occupy ...
Yang Liu +7 more
wiley +1 more source
POLYMORPHISM GENE OF CYTOCHROME-b (MT-CYB) MEDAKA FISH Oryzias celebensis ON SOME RIVERS IN SOUTH SULAWESI BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) METHOD [PDF]
\ud \ud The study titled Polymorphism Gene of Cytochrome-b (MT-CYB) Medaka Fish Oryzias celebensis On Some Rivers In South Sulawesi By Using Restriction fragment length polymorphism (RFLP) Method, has been performed.
Sastrawan, Sadhly
core
The regulation of stem cell fate and its application in neural regeneration
Regulating stem cell fate is crucial for neural regeneration. This review summarizes key physical, biological, and chemical strategies and their applications in repairing nerve injuries, providing new insights for regenerative medicine. Abstract Regulating the fate of stem cells (SCs) is a key technical problem in the field of regenerative medicine and
Yuexin He +3 more
wiley +1 more source
Search for Restriction Fragment Length Polymorphism of Phaseolus Vulgaris in Relation to the Immune Gene to Bean Common Mosaic Virus [PDF]
A technique involving Restriction Fragment Length Polymorphism (RFLP) was used to observe the DNA fragment polymorphism between a bean cultivar with I/I genotype and a bean cultivar with i/i genotype.
Masli, Aryananda
core
Reidite Formation From Zircon Subjected to 20, 40, and 60 GPa Shock Experiments
Structure disordering as a result of shock pressure: In zircon shocked from 20 to 60 GPa, the shock‐induced structural disorder increases with increasing shock pressure (gray area). Shock disorder of zircon is an integral part of its transformation to reidite: Reidite occurs after amorphous material.
Dmitry A. Zamyatin, Elizaveta Kovaleva
wiley +1 more source
Abstract BACKGROUND Rice milling characteristics and storage stability are critical traits influencing industrial applications. Floury endosperm rice carrying the flo4‐4 allele is valuable for dry milling but exhibits reduced storage stability associated with altered starch structural characteristics.
Su‐Kyung Ha +11 more
wiley +1 more source
Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential [PDF]
In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo.
Traub, R.J. +7 more
core +1 more source
Radiotherapy‐Induced Otitis Media With Effusion in Nasopharyngeal Carcinoma: A Meta‐Analysis
This meta‐analysis of eight studies comprising 582 NPC patients provides the first quantitative synthesis of radiotherapy‐induced otitis media with effusion (OME), demonstrating a significant overall risk difference of 0.18 (95% CI: −0.06 to 0.42, p < 0.0001) in OME naive ears.
Nevin Yi Meng Chua +3 more
wiley +1 more source
Ion Activation Methods for Top‐Down Proteomics
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source

