Results 171 to 180 of about 6,098 (217)

Cathepsin D: Screening for new polymorphisms using single-strand conformation polymorphism analysis

International Journal of Molecular Medicine, 2002
Cathepsin D (CTSD) is a lysosomal protease involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer's disease (AD). Previous findings revealed a significant association between the T allele of the 224 C/T (A58V) polymorphism in exon 2 of the CTSD gene and late onset AD.
Majores, Micael, Kolsch, Heike, Bagli, Metin, Papassotiropoulos, Andreas, Lohmann, Petra Louise, Schmitz, Sandra, Rao, Marie Luise, Maier, Wolfgang, Heun, Reinhard   +8 more
openaire   +2 more sources

Screening Hypermethylated Regions by Methylation-Sensitive Single-Strand Conformational Polymorphism

Analytical Biochemistry, 2000
DNA methylation is an important epigenetic modification that alters transcription in those genes containing CpG islands. In this report we demonstrate that the familiar technique single-strand conformation polymorphism (SSCP) can be easily applied to bisulfite-treated DNA to detect methylation differences over large ( approximately 250 bp) CG-enriched ...
H, Kinoshita, Y, Shi, C, Sandefur, D F, Jarrard   +3 more
openaire   +2 more sources

Analysis of single-strand DNA conformation polymorphism by capillary electrophoresis

Journal of Chromatography A, 1996
Analysis of single-strand conformation polymorphism (SSCP) by capillary electrophoresis (CE) was developed. The conformational change of single-strand DNA is caused by a mutation in a DNA fragment. The change is detected as mobility shift in CE.
H, Arakawa, S, Nakashiro, M, Maeda, A, Tsuji   +3 more
openaire   +2 more sources

Single-Strand Conformation Polymorphism Analysis

1996
Nucleotide sequences of genomic DNA in cells can be altered by a variety of factors. If the alterations do not influence the growth and development of cells, the nucleotide sequences present in germinal cells can be transferred to progenies. If the nucleotide sequence changes influence normal cellular functions, they result in diverse diseases, such as
openaire   +1 more source

Development of a Sensitive Deaminated Single-Strand Conformation Polymorphism (DSSCP)

Applied Biochemistry and Biotechnology, 2009
The single-strand conformation polymorphism (SSCP), accompanied by sequencing, is a useful methods for identifying mutations in a DNA fragment. In this study, we have developed a modified SSCP with the aid of sodium bisulfite treatment. The corresponding PCR products for exon 3 of Hb gene were sequenced and samples with homozygote and heterozygote ...
Sadeq, Vallian, Isar, Nassiri
openaire   +2 more sources

Single-stranded conformation polymorphism and heteroduplex analysis

1998
Abstract Single-stranded conformation polymorphism (SSCP) alone or in combination with heteroduplex analysis (HA) is one of the most widely used and practical approaches for mutation detection (1). The principal advantage of this method is that it is rapid to perform and can be carried out using equipment available in most molecular ...
Bernard Gerrard, Michael Dean
openaire   +1 more source

Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

2012
Single-strand conformation polymorphism (SSCP) analysis is a sensitive and rapid technique for detecting DNA polymorphisms and mutations in PCR-amplified fragments. Due to its technical simplicity, it is widely used as a screening tool in various investigations, ranging from clinical diagnosis of human hereditary diseases to the characterization of ...
Šeruga Musić, Martina, Škorić, Dijana   +1 more
openaire   +2 more sources

Human SP-A: genotype and single-strand conformation polymorphism analysis

American Journal of Physiology-Lung Cellular and Molecular Physiology, 1994
We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A1. These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination ...
L, Krizkova, R, Sakthivel, S A, Olowe, P K, Rogan, J, Floros   +4 more
openaire   +2 more sources

Rapid identification of bacteria by PCR-single-strand conformation polymorphism

Journal of Clinical Microbiology, 1994
A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation polymorphism (SSCP).
M N, Widjojoatmodjo, A C, Fluit, J, Verhoef   +2 more
openaire   +2 more sources

Identification of mycobacteria by nonradioisotopic single-strand conformation polymorphism analysis

Diagnostic Microbiology and Infectious Disease, 1995
Clinical isolates of mycobacteria were identified to species levels using nonradioisotopic single-strand conformation polymorphism (non-RI SSCP) analysis of 16S rRNA gene fragments amplified by polymerase chain reaction with primers common to all of mycobacterial species. The method is based on a hypervariable region within the 16S rRNA in mycobacteria,
Y, Tokue, K, Sugano, T, Noda, D, Saito, Y, Shimosato, H, Ohkura, T, Kakizoe, T, Sekiya   +7 more
openaire   +2 more sources