Results 201 to 210 of about 20,043 (225)

Analysis of single-strand DNA conformation polymorphism by capillary electrophoresis

Journal of Chromatography A, 1996
Analysis of single-strand conformation polymorphism (SSCP) by capillary electrophoresis (CE) was developed. The conformational change of single-strand DNA is caused by a mutation in a DNA fragment. The change is detected as mobility shift in CE.
H, Arakawa, S, Nakashiro, M, Maeda, A, Tsuji   +3 more
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Single-Strand Conformation Polymorphism Analysis

1996
Nucleotide sequences of genomic DNA in cells can be altered by a variety of factors. If the alterations do not influence the growth and development of cells, the nucleotide sequences present in germinal cells can be transferred to progenies. If the nucleotide sequence changes influence normal cellular functions, they result in diverse diseases, such as
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Development of a Sensitive Deaminated Single-Strand Conformation Polymorphism (DSSCP)

Applied Biochemistry and Biotechnology, 2009
The single-strand conformation polymorphism (SSCP), accompanied by sequencing, is a useful methods for identifying mutations in a DNA fragment. In this study, we have developed a modified SSCP with the aid of sodium bisulfite treatment. The corresponding PCR products for exon 3 of Hb gene were sequenced and samples with homozygote and heterozygote ...
Sadeq, Vallian, Isar, Nassiri
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Single-stranded conformation polymorphism and heteroduplex analysis

1998
Abstract Single-stranded conformation polymorphism (SSCP) alone or in combination with heteroduplex analysis (HA) is one of the most widely used and practical approaches for mutation detection (1). The principal advantage of this method is that it is rapid to perform and can be carried out using equipment available in most molecular ...
Bernard Gerrard, Michael Dean
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Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

2012
Single-strand conformation polymorphism (SSCP) analysis is a sensitive and rapid technique for detecting DNA polymorphisms and mutations in PCR-amplified fragments. Due to its technical simplicity, it is widely used as a screening tool in various investigations, ranging from clinical diagnosis of human hereditary diseases to the characterization of ...
Šeruga Musić, Martina, Škorić, Dijana   +1 more
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Human SP-A: genotype and single-strand conformation polymorphism analysis

American Journal of Physiology-Lung Cellular and Molecular Physiology, 1994
We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A1. These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination ...
L, Krizkova, R, Sakthivel, S A, Olowe, P K, Rogan, J, Floros   +4 more
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Rapid identification of bacteria by PCR-single-strand conformation polymorphism

Journal of Clinical Microbiology, 1994
A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation polymorphism (SSCP).
M N, Widjojoatmodjo, A C, Fluit, J, Verhoef   +2 more
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Identification of mycobacteria by nonradioisotopic single-strand conformation polymorphism analysis

Diagnostic Microbiology and Infectious Disease, 1995
Clinical isolates of mycobacteria were identified to species levels using nonradioisotopic single-strand conformation polymorphism (non-RI SSCP) analysis of 16S rRNA gene fragments amplified by polymerase chain reaction with primers common to all of mycobacterial species. The method is based on a hypervariable region within the 16S rRNA in mycobacteria,
Y, Tokue, K, Sugano, T, Noda, D, Saito, Y, Shimosato, H, Ohkura, T, Kakizoe, T, Sekiya   +7 more
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[11] Mutational detection by single-strand conformational polymorphism

1995
Summary In addition to providing valuable information on the possible location of mutations within particular genes, PCR-SSCP is a powerful method for converting random DNA fragments to polymorphic markers for linkage analysis. In addition, once a given mutation is identified, a specific oligonucleotide primer can be designed such that the 3′ most ...
Nabeel Bardeesy, Jerry Pelletier
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Single-Strand Conformational Polymorphism Analysis: Basic Principles and Routine Practice

2005
Single-strand conformational polymorphism (SSCP) analysis is a simple and sensitive technique for mutation detection and genotyping. The principle of SSCP analysis is based on the fact that single-stranded DNA has a defined conformation. Altered conformation due to a single base change in the sequence can cause single-stranded DNA to migrate ...
Yanbin, Dong, Haidong, Zhu
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