Results 121 to 130 of about 88,763 (155)
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Polynucleotide cellulose as a substrate for a polynucleotide ligase induced by phage T4
Biochemical and Biophysical Research Communications, 1967Abstract An enzyme which joins polydeoxynucleotide strands has been identified in and purified from extracts Escherichia coli infected with phage T4 am N82 and appears to be the same as the ligase described by Weiss and Richardson. A novel method for detecting and assaying such enzymes involves the joining of a strand to one that is ...
N R, Cozzarelli +3 more
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Changes in polynucleotide ligase during rat liver regeneration
Biochemical and Biophysical Research Communications, 1974Abstract The specific activity of polynucleotide ligase in rat liver seems to begin to rise at 16 hours after partial hepatectomy (removal of 70% of the liver). The increases reach their maxima about 24 hours after operation, rising to at least 4 to 5 fold normal levels.
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A new method of assay for polynucleotide ligase
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974Abstract A new method for the assay of polynucleotide ligase is described. It consists of two parallel DNA polymerase reactions with poly(dA) · oligo(dT) as template-primer and d[3H] TTP as precursor; ligase is added to both, but one is supplemented with NMN and the other with NAD.
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Intergenic suppression of amber polynucleotide ligase mutation in bacteriophage T4
Virology, 1970Abstract An intergenic suppressor ( m ) of the amber ligase mutation ( amH 39 x ) was isolated and a number of possible mechanisms of suppression were investigated. The intergenic suppressor does not appear to involve suppression of the amber codon.
V L, Chan, S, Shugar, K, Ebisuzaki
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Induction of polynucleotide ligase in human lymphocytes stimulated by phytohemoagglutinin
Biochemical and Biophysical Research Communications, 1972Abstract The treatment of human lymphocytes with phytohemoagglutinin causes the appearance of the activity of polynucleotide ligase, rising at least 50 fold from levels below the background. This increase takes place in the fourth or fifth day after treatment, and is delayed by one day approximately with respect to the rise of DNA synthesis rate; the
A M, Pedrini +4 more
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The activity of mammalian polynucleotide ligase on X-irradiated DNAs
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978Selected samples of heterogeneous DNA from calf thymus with similar number-average molecular weight, Mn, and a low incidence of single-strand breaks were exposed in aqueous solutions to a mild X-ray dose of 1500 rads. The irradiation produced on the average about 0.2 bihelical and 2.2 monohelical scissions per DNA molecule of 1708 000 Mn.
M, Mathelet +3 more
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Purification and properties of a polynucleotide ligase from calf thymus glands
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract Polynucleotide ligase has been purified over 390-fold from extracts of calf thymus glands. A solid-state model of DNA containing single-strand breaks, namely, the poly(dA) · [poly([ 3 H]dT)-poly(dT)]-cellulose, was used as substrate for the enzymatic assays.
BERTAZZONI, Umberto +2 more
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T4 polynucleotide ligase catalyzed joining on triple-stranded nucleic acids
Biochemistry, 1978dT1O will form triple-stranded complexes with dAn and these complexes can serve as substrate for T4 polynucleotide ligase (EC 6.5.1.1). The rate of phosphodiester formation was found to be approximately the same as for the double-stranded complex and, furthermore, the rate appears to be similar on the two strands in the complex.
A J, Raae, K, Kleppe
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Chemico-Biological Interactions, 1981
Conformationally distinct chromatin populations were utilized as substrates to quantitate the relative amount of and accessibility of internal 5'-phosphomonoester breaks in DNA-chromatin. In these studies, a constant amount of chromatin as well as deproteinized DNA derived from the respective chromatin sample was titrated with increasing quantities of ...
R A, Montagna +3 more
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Conformationally distinct chromatin populations were utilized as substrates to quantitate the relative amount of and accessibility of internal 5'-phosphomonoester breaks in DNA-chromatin. In these studies, a constant amount of chromatin as well as deproteinized DNA derived from the respective chromatin sample was titrated with increasing quantities of ...
R A, Montagna +3 more
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Solid-phase synthesis of oligoribonucleotides using T4 RNA ligase and T4 polynucleotide kinase
Biochimie, 1995The concept of solid-phase synthesis of oligoribonucleotides using T4 RNA ligase and T4 polynucleotide kinase has been proposed and tested with model homo-oligoribonucleotides. The method consists of the immobilization of the first oligomer block at the 3'-terminus on a solid support followed by a chain elongation in the 5'-direction with trinucleoside
L V, Vratskikh +2 more
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