Results 61 to 70 of about 3,900 (192)
Deinococcus radiodurans RNA ligase exemplifies a novel ligase clade with a distinctive N-terminal module that is important for 5′-PO(4) nick sealing and ligase adenylylation but dispensable for phosphodiester formation at an adenylylated nick [PDF]
, 2007 Deinococcus radiodurans RNA ligase (DraRnl) is a template-directed ligase that seals nicked duplexes in which the 3′-OH strand is RNA. DraRnl is a 342 amino acid polypeptide composed of a C-terminal adenylyltransferase domain fused to a distinctive 126 ...
Raymond, Amy, Shuman, Stewart
core +2 more sources
ChemCatChem, Volume 17, Issue 20, October 20, 2025.Thioxanthone is an efficient visible‐light photosensitizer and facilitates reactions that are not known from natural enzymes. It is now possible to incorporate thioxanthone as a noncanonical amino acid (thioX) into proteins by an engineered amino acyl tRNA synthetase.
Marco Seifert +3 more
wiley +1 more source
The Lrs14‐Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid‐Organizing Protein
MicrobiologyOpen, Volume 14, Issue 5, October 2025.Nucleoid organization in Crenarchaeota involves diverse small DNA‐binding proteins. The Lrs14‐type protein AbfR1 from Metallosphaera sedula binds nonsequence specifically across the genome and induces DNA condensation. These findings suggest a structural role for AbfR1Ms in chromatin architecture, functionally resembling bacterial nucleoid‐associated ...
Veerke De Kock +5 more
wiley +1 more source
Mechanisms of base selection by the E.coli mispaired uracil glycosylase [PDF]
, 2008 The repair of the multitude of single-base lesions formed daily in the cells of all living organisms is accomplished primarily by the base-excision repair (BER) pathway that initiates repair through a series of lesion-selective glycosylases.
Darwanto, Agus +6 more
core +2 more sources
High-efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing [PDF]
, 2013 Small RNA cloning and sequencing is uniquely positioned as a genome-wide approach to quantify miRNAs with single-nucleotide resolution. However, significant biases introduced by RNA ligation in current protocols lead to inaccurate miRNA quantification by
Emily M Anderson +4 more
core +1 more source
Autosomal Recessive Cerebellar Ataxias: Translating Genes to Therapies
Annals of Neurology, Volume 98, Issue 3, Page 448-470, September 2025.[Color figure can be viewed at www.annalsofneurology.org] Autosomal recessive cerebellar ataxias are disabling neurodegenerative genetic conditions affecting balance and coordination. Advancements in genomic testing have improved diagnosis, leading to a new focus on the development of targeted precision therapeutics addressing cellular, biochemical ...
Brent L. Fogel +10 more
wiley +1 more source
Mechanisms of DNA damage, repair, and mutagenesis [PDF]
, 2017 Living organisms are continuously exposed to a myriad of DNA damaging agents that can impact health and modulate disease-states. However, robust DNA repair and damage-bypass mechanisms faithfully protect the DNA by either removing or tolerating the ...
Chatterjee, Nimrat, Walker, Graham C
core +1 more source
CRISPR/Cas9‐Mediated Gene Knockout in Cereal Crops
Current Protocols, Volume 5, Issue 9, September 2025.Abstract High‐precision genome editing tools, such as programmable nucleases, are poised to transform crop breeding and significantly impact fundamental plant research. Among these tools, the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR‐associated 9) system is a programmable, RNA‐guided nuclease that introduces ...
Dibyajyoti Pramanik +2 more
wiley +1 more source
T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis [PDF]
, 2011 Background T4 RNA ligases 1 and 2 are useful tools for RNA analysis. Their use upstream of RNA analyses such as high-throughput RNA sequencing and microarrays has recently increased their importance.
Fuchs, Ryan T +4 more
core +3 more sources
Mammalian Base Excision Repair: the Forgotten Archangel [PDF]
, 2017 Base excision repair (BER) is a frontline repair system that is responsible for maintaining genome integrity and thus preventing premature aging, cancer and many other human diseases by repairing thousands of DNA lesions and strand breaks continuously ...
Dianov, Grigory L., Hübscher, Ulrich
core