Results 61 to 70 of about 44,353 (243)

Different csrA Expression Levels in C versus K-12 E. coli Strains Affect Biofilm Formation and Impact the Regulatory Mechanism Presided by the CsrB and CsrC Small RNAs

open access: yesMicroorganisms, 2021
Escherichia coli C is a strong biofilm producer in comparison to E. coli K-12 laboratory strains due to higher expression of the pgaABCD operon encoding the enzymes for the biosynthesis of the extracellular polysaccharide poly-β-1,6-N-acetylglucosamine ...
Thomas Carzaniga   +5 more
doaj   +1 more source

A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum [PDF]

open access: yes, 2011
Background Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C.
Fan, Keqiang   +6 more
core   +3 more sources

Lack of PNPase activity in Enterococcus faecalis 14 increases the stability of EntDD14 bacteriocin transcripts

open access: yesScientific Reports, 2023
A mutant deficient in polynucleotide phosphorylase (PNPase) activity was previously constructed in Enterococcus faecalis 14; a strain producing a leaderless two-peptide enterocin DD14 (EntDD14).
Rabia Ladjouzi   +3 more
doaj   +1 more source

Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs

open access: yesBio-Protocol, 2018
The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases.
Christian Beren   +4 more
doaj   +1 more source

Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized. [PDF]

open access: yes, 2018
In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial dysfunction to various diseases, and
Gammage, Payam A   +2 more
core   +1 more source

Rat liver mitochondrial polynucleotide phosphorylase

open access: yesFEBS Letters, 1971
We have recently isolated, partially purified and demonstrated the specificity of a polynucleotide phosphorylase from crude guinea-pig liver nuclei [ 1,2] The enzyme was associated with membrane lipids and catalysed the phosphorolysis of polyribonucleotides to ribonucleoside diphosphates.
See, Yew Phew, Fitt, P.S.
openaire   +2 more sources

Sequence motifs that distinguish ATP(CTP) : tRNA nucleotidyl transferases from eubacterial poly(A) polymerases [PDF]

open access: yes, 2004
ATP(CTP):tRNA nucleotidyl transferases, tRNA maturing enzymes found in all organisms, and eubacterial poly(A) polymerases, enzymes involved in mRNA degradation, are so similar that until now their biochemical functions could not be distinguished by their
Keller, W., Martin, G.
core   +2 more sources

RNase E biomolecular condensates stimulate PNPase activity

open access: yesScientific Reports, 2023
Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Michael J. Collins   +4 more
doaj   +1 more source

Insights into Xanthomonas axonopodis pv. citri biofilm through proteomics [PDF]

open access: yes, 2013
Background: Xanthomonas axonopodis pv. citri (X. a. pv. citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and
Garavaglia, Betiana Soledad   +6 more
core   +1 more source

Genome wide association studies for acid phosphatase activity at varying phosphorous levels in Brassica juncea L

open access: yesFrontiers in Plant Science, 2022
Acid phosphatases (Apases) are an important group of enzymes that hydrolyze soil and plant phosphoesters and anhydrides to release Pi (inorganic phosphate) for plant acquisition. Their activity is strongly correlated to the phosphorus use efficiency (PUE)
Priyanka Upadhyay   +7 more
doaj   +1 more source

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