Results 51 to 60 of about 11,317 (212)

ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES

Journal of Biological Chemistry, 1957
SUMMARY The relative activity toward different nucleoside diphosphates remains essentially unchanged after 500-fold purification of polynucleotide phosphorylase of Azotobacter v&elan&i suggesting that a single enzyme is involved. The purified enzyme, virtually free of nuclease, contains small amounts of an oligoribonucleotide (with adenine, guanine ...
Matthys Staehelin, Severo Ochoa, Dewey O. Brummond   +2 more
openaire   +3 more sources

Exploring the Potential and Advancements of Circular RNA Therapeutics

Exploration, EarlyView.
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang, Lianqing Wang, Chunbo Dong, Jialong Liu, Guoxin Cui, Shan Gao, Zhida Liu   +6 more
wiley   +1 more source

Polynucleotide-Chitosan Complex, an Insoluble but Reactive Form of Polynucleotide.

Chemical and Pharmaceutical Bulletin, 1997
DNA formed an insoluble complex on mixing with chitosan (poly-D-glucosamine) in solution. The DNA content of the complex was about 50% and the DNA remained insoluble in aqueous media of pH 2-7%; e.g., on treatment of the DNA-chitosan complex with phosphate-buffered saline at pH 7 and 37 degrees C for 26 h, the DNA released into the aqueous phase was ...
Takashi Kubo, Yuji Tanaka, Kazuo Negishi, Hikoya Hayatsu   +3 more
openaire   +3 more sources

Prediction of Proliferation Rate of Pre‐Osteoblasts Using Raman Spectroscopy and Machine Learning Models

Journal of Raman Spectroscopy, EarlyView.
The main processes involved in predicting the pre–osteoblast proliferation rate using Raman spectroscopy and machine learning. ABSTRACT This novel study focuses on using Raman spectroscopy and machine learning (ML) models to forecast the murine pre‐osteoblast (OB) proliferation rate.
Sai S. Sudha, Angshuman Baradwaz, Ahmad Y. Javaid, Ambalangodage C. Jayasuriya   +3 more
wiley   +1 more source

The transcription factor c-Myc suppresses MiR-23b and MiR-27b transcription during fetal distress and increases the sensitivity of neurons to hypoxia-induced apoptosis. [PDF]

PLoS ONE, 2015
Previous studies reported that the expression of miR-23b-27b cluster was downregulated in embryonic brain cortices during hypoxia-induced neuronal apoptosis. However, the mechanism underlying this downregulation is not completely understood.
Qun Chen, Fan Zhang, Yanbo Wang, Zhengya Liu, Anyang Sun, Ke Zen, Chen-yu Zhang, Qipeng Zhang   +7 more
doaj   +1 more source

Nanoparticle Therapeutics in Clinical Perspective: Classification, Marketed Products, and Regulatory Landscape

Small, EarlyView.
This review presents a detailed overview of clinically approved nanoparticle therapeutics, classifying them by type and discussing their unique advantages in drug delivery. It highlights regulatory challenges across global markets and emphasizes the need for adaptive approval pathways.
Nimeet Desai, Dhwani Rana, Mitali Patel, Neha Bajwa, Rajendra Prasad, Lalitkumar K. Vora   +5 more
wiley   +1 more source

Concentration and isolation of DNA from biological fluids by agarose gel isotachophoresis

BioTechniques, 2005
Isotachophoresis is an electrophoretic method of separation of charged substances. The method is characterized by a discontinuous buffer system, constant velocity of separated molecules, and the distribution of separated components in the form of narrow ...
Valentina N. Kondratova, Ol'ga I. Serd'uk, Valery P. Shelepov, Anatoly V. Lichtenstein   +3 more
doaj   +1 more source

A novel DNA vaccine technology conveying protection against a lethal herpes simplex viral challenge in mice. [PDF]

PLoS ONE, 2013
While there are a number of licensed veterinary DNA vaccines, to date, none have been licensed for use in humans. Here, we demonstrate that a novel technology designed to enhance the immunogenicity of DNA vaccines protects against lethal herpes simplex ...
Julie L Dutton, Bo Li, Wai-Ping Woo, Joshua O Marshak, Yan Xu, Meei-li Huang, Lichun Dong, Ian H Frazer, David M Koelle   +8 more
doaj   +1 more source

The Reaction of Formaldehyde with Polynucleotides

Journal of Biological Chemistry, 1961
Formaldehyde has been used extensively for chemically modifying proteins and for inactivating toxins and viruses, although the precise mode of its action is little understood. In the case of tobacco mosaic virus, however, Fraenkel-Conrat (1) and Staehelin (2) have shown that inactivation of the virus is due to interaction of formaldehyde with the amino
Robert Haselkorn, Paul Doty
openaire   +3 more sources

Organic Reactivity Matters for the Emergence of Life: Kinetic Barriers and Molecular Diversity Are Suggested as Crucial Factors by Emerging Autonomous System Models

ChemSystemsChem, EarlyView.
Self‐organisation may result from organic systems fed with free energy provided that reactivity becomes a determining factor. The propensity of carbon to form covalent bonds can give rise to kinetically stable thermodynamically activated compounds possibly undergoing non‐linear kinetic processes.
Robert Pascal
wiley   +1 more source