Results 51 to 60 of about 27,712 (217)
ChemCatChem, EarlyView.Thioxanthone is an efficient visible‐light photosensitizer and facilitates reactions that are not known from natural enzymes. It is now possible to incorporate thioxanthone as a noncanonical amino acid (thioX) into proteins by an engineered amino acyl tRNA synthetase.
Marco Seifert +3 more
wiley +1 more source
Ribozyme Activity of RNA Nonenzymatically Polymerized from 3′,5′-Cyclic GMP
Entropy, 2013 3′,5′-Cyclic GMP spontaneously nonenzymatically polymerizes in a base-catalyzed reaction affording G oligonucleotides. When reacted with fully or partially sequence-complementary RNA (oligo C), the abiotically generated oligo G RNA displays a typical ...
Samanta Pino +5 more
doaj +1 more source
A personal 360° view of applications of ‘biomimetic’ molecular recognition
Journal of Chemical Technology &Biotechnology, EarlyView.Abstract Molecular recognition between biological molecules has formed the basis for innumerable applications in biotechnology for the last seven decades or so. Techniques such as affinity chromatography, solid‐phase and aqueous two‐phase extraction, affinity precipitation, biomimetic catalytic systems, biosensors and molecular imprinting all exploit ...
Christopher R Lowe
wiley +1 more source
Translocation of structured polynucleotides through nanopores [PDF]
Physical Biology, 2003 We investigate theoretically the translocation of structured RNA/DNA molecules through narrow pores which allow single but not double strands to pass. The unzipping of basepaired regions within the molecules presents significant kinetic barriers for the ...
U. Gerland, R. Bundschuh, T. Hwa
semanticscholar +1 more source
The FEBS Journal, EarlyView.When overexpressed in Staphylococcus aureus, the type I toxins SprA1 and SprA2 form membrane pores, with SprA1 creating stable pores and SprA2 forming transient ones. Both induce concomitant membrane depolarization, ATP release, and growth arrest, while only SprA1 causes membrane permeabilization due to its stable pore formation.
Laurence Fermon +10 more
wiley +1 more source
Study on Interaction of Cationic Porphyrazine with Synthetic Polynucleotides
Analytical Cellular Pathology, 2013 Interactions of cationic tetrakis (N, N′, N″, N‴- tetramethyltetra-3, 4-pyridinoporphyrazinatozinc (II) (Zn (tmtppa)) with synthetic polynucleotides, poly (G-C) and poly (A-T), and calf thymus DNA have been characterized in 7.5 mM phosphate buffer of pH ...
Hamid Dezhampanah, Soghra Fyzolahjani
doaj +1 more source
The transcription factor c-Myc suppresses MiR-23b and MiR-27b transcription during fetal distress and increases the sensitivity of neurons to hypoxia-induced apoptosis. [PDF]
PLoS ONE, 2015 Previous studies reported that the expression of miR-23b-27b cluster was downregulated in embryonic brain cortices during hypoxia-induced neuronal apoptosis. However, the mechanism underlying this downregulation is not completely understood.
Qun Chen +7 more
doaj +1 more source
Human FASTK preferentially binds single‐stranded and G‐rich RNA
The FEBS Journal, EarlyView.Although FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Daria M. Dawidziak +7 more
wiley +1 more source
Concentration and isolation of DNA from biological fluids by agarose gel isotachophoresis
BioTechniques, 2005 Isotachophoresis is an electrophoretic method of separation of charged substances. The method is characterized by a discontinuous buffer system, constant velocity of separated molecules, and the distribution of separated components in the form of narrow ...
Valentina N. Kondratova +3 more
doaj +1 more source
A novel DNA vaccine technology conveying protection against a lethal herpes simplex viral challenge in mice. [PDF]
PLoS ONE, 2013 While there are a number of licensed veterinary DNA vaccines, to date, none have been licensed for use in humans. Here, we demonstrate that a novel technology designed to enhance the immunogenicity of DNA vaccines protects against lethal herpes simplex ...
Julie L Dutton +8 more
doaj +1 more source