Results 51 to 60 of about 1,013 (157)

Protein purification via consecutive histidine–polyphosphate interaction

open access: yesProtein Science, Volume 33, Issue 6, June 2024.
Abstract While nickel‐nitrilotriacetic acid (Ni‐NTA) has greatly advanced recombinant protein purification, its limitations, including nonspecific binding and partial purification for certain proteins, highlight the necessity for additional purification such as size exclusion and ion exchange chromatography.
Zihao Zhou   +3 more
wiley   +1 more source

InflammamiRs in focus: Delivery strategies and therapeutic approaches

open access: yesThe FASEB Journal, Volume 38, Issue 5, 15 March 2024.
microRNAs (miRNAs) are non‐coding RNAs which regulate gene expression at the post‐transcriptional level. Dysregulated expression of miRNAs has been linked to the pathogenesis of inflammatory diseases. miRNAs that play a role in inflammatory pathways have been termed inflammamiRs.
Yeliz Z. Akkaya‐Ulum   +3 more
wiley   +1 more source

Application of enzymatic activity measurements to biological phosphate removal from wastewater

open access: yes, 1997
Activity measurements of enzymes have been used in the research. Properties of microorganism populations of activated sludge have been estimated by this method.
Andreas Franz   +3 more
core   +1 more source

Visible Light Activates Coumarin‐Caged mRNA for Cytoplasmic Cap Methylation in Cells

open access: yesChemistry – A European Journal, Volume 30, Issue 2, January 8, 2024.
A 5′ cap with coumarin at the N7G was prepared in two different ways. It can be used to make mRNAs and to activate the process of cytoplasmic cap methylation in cells by visible light. Abstract Protein synthesis is important and regulated by various mechanisms in the cell.
Amarnath Bollu   +5 more
wiley   +1 more source

THE STAINING OF TRIPHOSPHATASES BY THE CHELATE REMOVAL METHOD

open access: yes, 1964
In the histochemical staining of enzymes by the chelate removal method, the substrate is a chelate, and the enzyme unmasks bound ions which are precipitated by suitable capturing ions in the incubating mixture.
GEORGE G. BERG
core   +1 more source

Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants

open access: yes, 2019
International audienceEukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF).
Hothorn, M.   +33 more
core   +1 more source

Inositol phosphatase INPP4B sustains ILC1s and intratumoral NK cells through an AKT-driven pathway [PDF]

open access: yes
Innate lymphoid cells (ILCs) are a heterogeneous population of lymphocytes that coordinate early immune responses and maintain tissue homeostasis. Type 1 innate immune responses are mediated by natural killer (NK) cells and group 1 ILCs (ILC1s).
Sudan, Raki   +8 more
core   +1 more source

The Ubiquitin Ligase Rsp5p is Required for the Modification and Sorting of Biosynthetic Membrane Proteins into Multivesicular Bodies in S. cerevisiae. [PDF]

open access: yes, 2004
Precursor forms of vacuolar proteins with transmembrane domains, such as the carboxypeptidase S Cps1p and the polyphosphatase Phm5p, are selectively sorted in endosomal compartments to vesicles that invaginate, budding into the lumen of the late ...
Haguenauer-Tsapis, Rosine   +3 more
core   +1 more source

Deciphering interplay between Salmonella invasion effectors. [PDF]

open access: yes, 2008
Bacterial pathogens have evolved a specialized type III secretion system (T3SS) to translocate virulence effector proteins directly into eukaryotic target cells.
Robert J Cain   +15 more
core   +2 more sources

Major contribution of transcription initiation to 5’-end formation of mitochondrial steady-state transcripts in maize

open access: yes, 2018
In plant mitochondria, some steady-state transcripts contain primary 5ʹ ends derived from transcription initiation, while the others have processed 5ʹ termini generated by post-transcriptional processing.
Tengxiang Lian (6156227)   +6 more
core   +1 more source

Home - About - Disclaimer - Privacy