Results 21 to 30 of about 6,340 (193)

Structural Determinants Responsible for Substrate Recognition and Mode of Action in Family 11 Polysaccharide Lyases [PDF]

open access: hybridJournal of Biological Chemistry, 2009
A saprophytic Bacillus subtilis secretes two types of rhamnogalacturonan (RG) lyases, endotype YesW and exotype YesX, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 YesW and YesX with a significant sequence identity (67.8%) cleave glycoside bonds between rhamnose ...
Akihito Ochiai   +4 more
openalex   +5 more sources

Genome Analysis of a Novel Polysaccharide-Degrading Bacterium Paenibacillus algicola and Determination of Alginate Lyases

open access: yesMarine Drugs, 2022
Carbohydrate-active enzymes (CAZymes) are an important characteristic of bacteria in marine systems. We herein describe the CAZymes of Paenibacillus algicola HB172198T, a novel type species isolated from brown algae in Qishui Bay, Hainan, China.
Huiqin Huang   +7 more
doaj   +1 more source

Crystal Structure of Family 14 Polysaccharide Lyase with pH-dependent Modes of Action [PDF]

open access: hybridJournal of Biological Chemistry, 2009
The Chlorella virus enzyme vAL-1 (38 kDa), a member of polysaccharide lyase family 14, degrades the Chlorella cell wall by cleaving the glycoside bond of the glucuronate residue (GlcA) through a beta-elimination reaction. The enzyme consists of an N-terminal cell wall-attaching domain (11 kDa) and a C-terminal catalytic module (27 kDa).
Kohei Ogura   +5 more
openalex   +5 more sources

Gellan lyases-novel polysaccharide lyases [PDF]

open access: yesMicrobiology, 1994
A number of bacterial strains capable of degrading the bacterial exopolysaccharide gellan have been isolated by standard enrichment procedures. They include several pink-pigmented Gram-negative rod-shaped bacteria. A red-pigmented Gram-positive bacillus earlier found to degrade the exopolysaccharide xanthan from Xanthomonas campestris also showed ...
Lynn Kennedy, Ian W. Sutherland
openaire   +3 more sources

Structural and Biochemical Analysis Reveals Catalytic Mechanism of Fucoidan Lyase from Flavobacterium sp. SA-0082

open access: yesMarine Drugs, 2022
Fucoidans represent a type of polyanionic fucose-containing sulfated polysaccharides (FCSPs) that are cleaved by fucoidan-degrading enzymes, producing low-molecular-weight fucoidans with multiple biological activities suitable for pharmacological use ...
Juanjuan Wang   +7 more
doaj   +1 more source

Structural insights into the mechanism of pH-selective substrate specificity of the polysaccharide lyase Smlt1473 [PDF]

open access: yesJournal of Biological Chemistry, 2021
Polysaccharide lyases (PLs) are a broad class of microbial enzymes that degrade anionic polysaccharides. Equally broad diversity in their polysaccharide substrates has attracted interest in biotechnological applications such as biomass conversion to value-added chemicals and microbial biofilm removal.
Shubhant Pandey   +5 more
openaire   +3 more sources

Phylogenetic Distribution of Polysaccharide-Degrading Enzymes in Marine Bacteria

open access: yesFrontiers in Microbiology, 2021
Deconstruction is an essential step of conversion of polysaccharides, and polysaccharide-degrading enzymes play a key role in this process. Although there is recent progress in the identification of these enzymes, the diversity and phylogenetic ...
Zhong-Zhi Sun   +12 more
doaj   +1 more source

Genome-Wide Analysis of PL7 Alginate Lyases in the Genus Zobellia

open access: yesMolecules, 2021
We carried out a detailed investigation of PL7 alginate lyases across the Zobellia genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to
Nadezhda Chernysheva   +4 more
doaj   +1 more source

Polysaccharide lyases from gellan-producing Sphingomonas spp. [PDF]

open access: yesMicrobiology, 1996
A number ofSphingomonasstrains capable of synthesizing the bacterial exopolysaccharide gellan and related polymers were shown to possess constitutive gellanase activity. In each case, the degradation of deacylated gellan was due to extracellular, eliminase-type enzymes (lyases) which cleave the sequence …ß-D-glucosyl 1,4-ß-D-glucuronosyl… in the ...
Ian W. Sutherland, Lynn Kennedy
openaire   +3 more sources

Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

open access: yesBiotechnology for Biofuels, 2020
Background As the most abundant polysaccharide in brown algae, alginate has become a promising economical material for bioethanol production. Recently, exo-type alginate lyases have received extensive attention because the unsaturated monosaccharides ...
Luyao Tang   +6 more
doaj   +1 more source

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