Polysaccharide utilization loci in Bacteroides determine population fitness and community-level interactions. [PDF]
Cell Host Microbe, 2022 Polysaccharide utilization loci (PULs) are co-regulated bacterial genes that sense nutrients and enable glycan digestion. Human gut microbiome members, notably Bacteroides, contain numerous PULs that enable glycan utilization and shape ecological dynamics.
Feng J +9 more
europepmc +7 more sources
Multi-omic Directed Discovery of Cellulosomes, Polysaccharide Utilization Loci, and Lignocellulases from an Enriched Rumen Anaerobic Consortium. [PDF]
Appl Environ Microbiol, 2020 The lignocellulolytic ERAC displays a unique set of plant polysaccharide-degrading enzymes (with multimodular characteristics), cellulosomal complexes, and PULs. The MAGs described here represent an expansion of the genetic content of rumen bacterial genomes dedicated to plant polysaccharide degradation, therefore providing a valuable resource for the ...
Tomazetto G +4 more
europepmc +7 more sources
Polysaccharide Utilization Loci: Fueling Microbial Communities. [PDF]
J Bacteriol, 2017 ABSTRACT The complex carbohydrates of terrestrial and marine biomass represent a rich nutrient source for free-living and mutualistic microbes alike. The enzymatic saccharification of these diverse substrates is of critical importance for fueling a variety of complex microbial communities, including marine, soil, ruminant, and ...
Grondin JM +4 more
europepmc +7 more sources
PULDB: the expanded database of Polysaccharide Utilization Loci. [PDF]
Nucleic Acids Res, 2018 The Polysaccharide Utilization Loci (PUL) database was launched in 2015 to present PUL predictions in ∼70 Bacteroidetes species isolated from the human gastrointestinal tract, as well as PULs derived from the experimental data reported in the literature.
Terrapon N +6 more
europepmc +10 more sources
Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans. [PDF]
Biotechnol Biofuels, 2021 AbstractBackgroundNowadays there is a strong trend towards a circular economy using lignocellulosic biowaste for the production of biofuels and other bio-based products. The use of enzymes at several stages of the production process (e.g., saccharification) can offer a sustainable route due to avoidance of harsh chemicals and high temperatures.
Hameleers L +9 more
europepmc +14 more sources
Research advances in polysaccharide utilization loci of rumen microorganism
浙江大学学报. 农业与生命科学版, 2020 Polysaccharide utilization loci (PULs) are gene clusters that orchestrate the breakdown of a specific glycan, encode cell surface polysaccharide binding proteins, outer membrane transport proteins, carbohydrate-active enzymes (CAZymes) and transcription ...
GAO Ge, WANG Jiakun
doaj +3 more sources
Small RNAs Repress Expression of Polysaccharide Utilization Loci of Gut Bacteroides Species. [PDF]
J Bacteriol, 2016 ABSTRACT Bacteroides species can metabolize numerous plant polysaccharides and host glycans present in the mammalian gut. The regulatory systems governing the induction of particular polysaccharide utilization loci when the cognate glycan is present are known, but how expression is repressed when a higher-priority ...
Comstock LE.
europepmc +5 more sources
Metagenomics reveals functional synergy and novel polysaccharide utilization loci in the Castor canadensis fecal microbiome. [PDF]
ISME J, 2018 Abstract The North American beaver (Castor canadensis) has long been considered an engineering marvel, transforming landscapes and shaping biological diversity through its dam building behavior. While the beaver possesses conspicuous morphological features uniquely adapted for the use of woody plants as construction materials and dietary
Armstrong Z +9 more
europepmc +5 more sources
Polysaccharide utilization loci of North Sea Flavobacteriia as basis for using SusC/D-protein expression for predicting major phytoplankton glycans. [PDF]
ISME J, 2019 Abstract Marine algae convert a substantial fraction of fixed carbon dioxide into various polysaccharides. Flavobacteriia that are specialized on algal polysaccharide degradation feature genomic clusters termed polysaccharide utilization loci (PULs). As knowledge on extant PUL diversity is sparse, we sequenced the genomes of 53 North Sea
Kappelmann L +10 more
europepmc +6 more sources
Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds. [PDF]
Appl Environ MicrobiolABSTRACT Lignocellulose is a renewable but complex material exhibiting high recalcitrance to enzymatic hydrolysis, which is attributed, in part, to the presence of covalent linkages between lignin and polysaccharides in the plant cell wall. Glucuronoyl esterases from carbohydrate esterase family 15 (CE15) have been proposed as an
Seveso A +5 more
europepmc +6 more sources