Results 81 to 90 of about 2,648 (172)

Exploring the Potential and Advancements of Circular RNA Therapeutics

Exploration, Volume 5, Issue 4, August 2025.
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang, Lianqing Wang, Chunbo Dong, Jialong Liu, Guoxin Cui, Shan Gao, Zhida Liu   +6 more
wiley   +1 more source

A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae

The Plant Journal, Volume 122, Issue 6, June 2025.
Significance Statement Protein tags for detection, purification and fluorescence labelling are vital in microalgal research, yet the suitability of specific tags remains largely unexplored. This review discusses the pros and cons of a comprehensive list of protein tags and provides important guidance for experimental design within a microalgal context.
Jonathan Scarfe, Darius Kosmützky, R. Ellen R. Nisbet   +2 more
wiley   +1 more source

Endocytic turnover of Rab8 controls cell polarization [PDF]

, 2017
Adaptation of cell shape and polarization through the formation and retraction of cellular protrusions requires balancing of endocytosis and exocytosis combined with fine-tuning of the local activity of small GTPases like Rab8.
Francis, Monika K., Hachimi, Mariam, Holst, Mikkel R., Larsson, Elin, Lundmark, Richard, Martin-Belmonte, Fernando, Peranen, Johan, Vidal-Quadras, Maite, Yau, Wai-Lok   +8 more
core   +1 more source

Autologous Peripheral Vγ9Vδ2 T Cell Synergizes with αβ T Cell Through Antigen Presentation and BTN3A1 Blockade in Immunotherapy of Cervical Cancer

Advanced Science, Volume 12, Issue 18, May 15, 2025.
This study provides proof‐of‐concept evidence showing that through direct killing, antigen presentation, and competitively binding to BTN3A1, Vγ9Vδ2 T cells amplified from cervical cancer (CC) patients can synergize with αβ T cells to exert anti‐CC function both in vitro and in vivo.
Min Wu, Jian Liu, Liting Liu, Yifan Yang, Hong Liu, Long Yu, Haihong Zeng, Shuo Yuan, Ruiyi Xu, Hangyu Liu, Han Jiang, Shen Qu, Liming Wang, Ying Chen, Jingyu Wang, Yuwei Zhang, Shan He, Ling Feng, Junyan Han, Wanjiang Zeng, Hui Wang, Yafei Huang   +21 more
wiley   +1 more source

A system for production of defective interfering particles in the absence of infectious influenza A virus

, 2019
Influenza A virus (IAV) infection poses a serious health threat and novel antiviral strategies are needed. Defective interfering particles (DIPs) can be generated in IAV infected cells due to errors of the viral polymerase and may suppress spread of wild
Arora, P., Bdeir, N., Gärtner, S., Hoffmann, M., Pöhlmann, S., Reichl, U., Winkler, M.   +6 more
core   +2 more sources

Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells [PDF]

, 2017
The expression of p63 in surface ectodermal cells during development of the cornea, skin, oral mucosa and olfactory placodes is integral to the process of cellular self-renewal and the maintenance of the epithelial stem cell status.
Hayashi, R., Kobayashi, Y., Nishida, K., Quantock, Andrew J.   +3 more
core   +3 more sources

Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ+ Cell Ablation in Adult Mice

Journal of Neurochemistry, Volume 169, Issue 3, March 2025.
Pericytes are essential for central nervous system vascular function, yet studying their role in adult pathologies has been challenging due to the scarcity of well‐characterized models that preserve normal vascular development. A tamoxifen‐inducible pericyte ablation model was developed to investigate pericyte dynamics after the acute loss of pericytes.
Dila Atak, Erdost Yıldız, Esra Özkan, Mohammadreza Yousefi, Ayşe Özkan, Aysu Bilge Yılmaz, Ali Burak Kızılırmak, Iman Asaad Alnajjar, Çiçek Kanar, Zeynep Lal Caan, Şakir Ümit Zeybek, Cem İsmail Küçükali, Erdem Tüzün, Yasemin Gürsoy‐Özdemir, Atay Vural   +14 more
wiley   +1 more source

Doenças do sistema nervoso central de suínos no sul do Brasil [PDF]

, 2021
Brazil is the fourth largest exporter of pork worldwide, with its production concentrated in the Southern region of the country. To reduce the economic impacts and increase herd health, an efficient diagnosis of diseases is necessary.
Almeida, Bruno Albuquerque de, Driemeier, David, Hammerschmitt, Marcia Elisa, Pavarini, Saulo Petinatti, Pereira, Paula Reis, Sonne, Luciana   +5 more
core  

Biomimetic Triplet Messenger RNA Formulation in MXene Hydrogel Microneedles for Wound Healing

Aggregate, Volume 6, Issue 2, February 2025.
The current direction in the messenger RNA (mRNA) field is focused on imparting mRNA with competitive synthetic functions and delicating carrier systems. By leveraging advanced artificial intelligence technology, a novel triplet mRNA formulation has been developed and delivered via photothermal‐responsive microneedles for the treatment of diabetic ...
Ji Wang, Yu Wang, Minhui Lu, Xinyue Cao, Ming Xia, Miaoqing Zhao, Yuanjin Zhao   +6 more
wiley   +1 more source

Rapid generation of drug-resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis [PDF]

, 2017
Genetic alterations conferring resistance to the effects of chemical inhibitors are valuable tools for validating on-target effects in cells. Unfortunately, for many therapeutic targets such alleles are not available.
Arai, E., Ipsaro, J. J., Joshua-Tor, L., Kinney, J. B., Shen, C., Shi, J., Vakoc, C. R., Xu, Y.   +7 more
core   +1 more source