Results 171 to 180 of about 2,754 (203)

Isolation and characterization of microsatellites in Portunus trituberculatus

Conservation Genetics Resources, 2011
Thirty polymorphic microsatellite markers for swimming crab P. trituberculatus are developed from EST sequences, genomic libraries and cross-species amplification in P. pelagicus, respectively. They are characterized using 24 P. trituberculatus individuals from Yingkou natural population.
Zhaoxia Cui, Yuan Liu, Hongxia Wang, Danhua Wu, Weisha Luan, Feng Tan, Minde Huang   +6 more
openaire   +1 more source

Bioconversion of progesterone by the ovaries of crab, Portunus trituberculatus

General and Comparative Endocrinology, 1971
Abstract In vitro bioconversion of progesterone-4- 14 C by the sliced ovaries of the crab Portunus trituberculatus has been investigated. The ovaries of the crab converted progesterone to 17α-hydroxyprogesterone, testosterone, and deoxycorticosterone.
S, Teshima, A, Kanazawa
openaire   +2 more sources

Gut Microbiota and Metabolic Phenotype of Portunus Trituberculatus

Chinese Journal of Analytical Chemistry, 2016
16S rRNA gene high-throughput sequencing and nuclear magnetic resonance (NMR)-based metabolomics techniques were established for the composition analysis of gut microbiota and metabolites of Portunus trituberculatus. Swimming crab gut samples were used for the extraction of bacterial genomic DNA, followed by amplification of hypervariable domain V3-V4 ...
Ting-Lan ZENG, Yang-Fang YE, Chang-Kao MU, Kai WANG, Rong-Hua LI, Chun-Lin WANG   +5 more
openaire   +1 more source

Identification of sex-linked DNA markers by AFLP in Portunus trituberculatus

Gene, 2014
To investigate the mechanism of sex determination of crabs, amplified fragment-length polymorphism DNA analysis was applied to identify sex-specific markers for Portunus trituberculatus. Eleven pairs of primers were used to amplify DNA isolated from male and female crabs.
XiaoFang, Lai, Huan, Gao, Binlun, Yan, Wenhua, Yan, Shanrui, Shen   +4 more
openaire   +2 more sources

Rapid immunochromatographic test strip to detect swimming crab Portunus trituberculatus reovirus

Diseases of Aquatic Organisms, 2015
Swimming crab reovirus (SCRV) is the causative agent of a serious disease with high mortality in cultured Portunus trituberculatus. A rapid immunochromatographic assay (ICA) was developed in a competitive assay format and optimized for the detection of SCRV.
LiPing, Zhang, DengFeng, Li, LianGuo, Liu, Ge, Zhang   +3 more
openaire   +2 more sources

Immune response and antioxidant status of Portunus trituberculatus inoculated with pathogens

Fish & Shellfish Immunology, 2017
The white spot syndrome virus (WSSV), Vibrio parahaemolyticus and V. alginolyticus are serious epidemic pathogen affecting the cultured Portunus trituberculatus and resulted in severe economic losses to local farmers. The immune and antioxidant systems are believed to be closely involved in host responses to pathogens in aerobic animals, including ...
Xianyun, Ren, Jianjian, Lv, Baoquan, Gao, Jian, Li, Ping, Liu   +4 more
openaire   +2 more sources

Quality changes in gazami crab (Portunus trituberculatus) during refrigeration

Food Chemistry
Gazami crab (Portunus trituberculatus) is prone to spoilage during storage and transportation. More research is needed to determine how to reliably show its freshness and explain the mechanism of quality deterioration. We hypothesized that proteins extracted from crabs can be biomarkers to detect crab muscle quality changes.
Yanmei, Ren, Jianan, Sun, Xiangzhao, Mao
openaire   +2 more sources

Dietary lysine requirement of juvenile swimming crab, Portunus trituberculatus

Aquaculture, 2015
Abstract An 8-week feeding trial was conducted to determine the optimal dietary lysine requirement for juvenile swimming crab reared in cement pools. Six iso-nitrogenous and iso-lipidic experimental diets (49.5% crude protein and 7.0% crude lipid) were formulated to contain graded levels of lysine, which were 1.58%, 1.89%, 2.15%, 2.41%, 2.67% and 2 ...
Min Jin, Meng-Qiang Wang, Ya-Wen Huo, Wen-Wen Huang, Kang-Sen Mai, Qi-Cun Zhou   +5 more
openaire   +1 more source

Expression and functional analysis of cytoplasmic dynein during spermatogenesis in Portunus trituberculatus

Cell and Tissue Research, 2021
The mechanism of acrosome formation in the crab sperm is a hot topic in crustacean reproduction research. Dynein is a motor protein that performs microtubule-dependent retrograde transport and plays an essential role in spermatogenesis. However, whether cytoplasmic dynein participates in acrosome formation in the crab sperm remains poorly understood ...
Chao-Guang Wei, Dan-Li Mu, Dao-Jun Tang, Jun-Quan Zhu, Cong-Cong Hou   +4 more
openaire   +2 more sources

Dynamic metabolite alterations of Portunus trituberculatus during larval development

Journal of Oceanology and Limnology, 2018
A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insufficient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs.
Ce Shi, Tinglan Zeng, Ronghua Li, Chunlin Wang, Yangfang Ye, Changkao Mu   +5 more
openaire   +1 more source