Results 191 to 200 of about 1,927,312 (260)

Cathepsin L-dependent positive selection shapes clonal composition and functional fitness of CD4⁺ T cells. [PDF]

Nat Immunol
Petrozziello E, Sayed A, Freitas JA, Federle C, Nedjic J, Ravens S, Akçabozan B, Schulz AM, Zehn D, Schmidt-Supprian M, Obst R, Prinz I, Verdoes M, Kisielow J, Reinheckel T, Straub T, Daley SR, Klein L.   +17 more
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Phylogenetic analysis and detection of positive selection in the SIRT gene family across vertebrates. [PDF]

Sci Rep
Zhou Q, Yang X, Li D, Li J, Peng L, He W.   +5 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Positive selection and relaxed purifying selection contribute to rapid evolution of sex-biased genes in green seaweed Ulva. [PDF]

BMC Ecol Evol
Liu X, Blomme J, Bogaert KA, D'hondt S, Coulembier Vandelannoote E, Wichard T, De Clerck O.   +6 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Single-cell DNA sequencing reveals pervasive positive selection throughout preleukemic evolution. [PDF]

Cell Genom
Poon G, Vedi A, Sanders M, Laurenti E, Valk P, Blundell JR.   +5 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Positive Selection of a Starch Synthesis Gene and Phenotypic Differentiation of Starch Accumulation in Symbiotic and Free-Living Coral Symbiont Dinoflagellate Species. [PDF]

Genome Biol Evol
Ishii Y, Kanamori S, Deguchi R, Kawata M, Maruyama S, Yoshida T, Kamikawa R.   +6 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source