Results 51 to 60 of about 3,016,110 (399)

Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes [PDF]

, 2002
Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of ...
Buitenweg, Jan Reinoud, Marani, Enrico, Rutten, Wim L.C.   +2 more
core   +3 more sources

MJK2, a K+Channel from M. Jannaschii Mediates pH Dependent Potassium Transport Activity [PDF]

, 2014
MjK2 was expressed in E. coli cells as a fusion protein containing N-or C-terminal an antibody binding site and a histidinehexamer. The C-terminal tagged fusion protein allows the expression and purification of an extra soluble RCK domain at p34 kDa ...
Zeilinger, Carsten
core   +2 more sources

A hydrophobic barrier deep within the inner pore of the TWIK-1 K2P potassium channel

Nature Communications, 2014
Recent X-ray crystal structures of the two-pore domain (K2P) family of potassium channels have revealed a unique structural architecture at the point where the cytoplasmic bundle-crossing gate is found in most other tetrameric K+ channels.
Prafulla Aryal, Firdaus Abd-Wahab, Giovanna Bucci, M. Sansom, S. Tucker   +4 more
semanticscholar   +1 more source

Nitric Oxide and Small and Intermediate Calcium-Activated Potassium Channels Mediate the Vasodilation Induced by Apigenin in the Resistance Vessels of Hypertensive Rats

Molecules
Background: Apigenin (4′,5,7-trihydroxyflavone), a flavonoid with potential cardiovascular benefits, has unclear mechanisms of action. This study investigates its effects on vascular function in Spontaneously Hypertensive Rats (SHRs). Methods: Mesenteric
Lislaine Maria Klider, Maria Luiza Fidelis da Silva, Gustavo Ratti da Silva, João Ricardo Cray da Costa, Marcia Alessandra Arantes Marques, Emerson Luiz Botelho Lourenço, Francislaine Aparecida dos Reis Lívero, Jane Manfron, Arquimedes Gasparotto Junior   +8 more
doaj   +1 more source

Single-channel recordings from cultured human retinal pigment epithelial cells. [PDF]

, 1988
We have applied patch-clamp techniques to on-cell and excised-membrane patches from human retinal pigment epithelial cells in tissue culture. Single-channel currents from at least four ion channel types were observed: three or more potassium-selective ...
Fain, GL, Fox, JA, Pfeffer, BA
core  

Selective disruption of high sensitivity heat activation but not capsaicin activation of TRPV1 channels by pore turret mutations. [PDF]

, 2012
The capsaicin receptor transient receptor potential vanilloid (TRPV)1 is a highly heat-sensitive ion channel. Although chemical activation and heat activation of TRPV1 elicit similar pungent, painful sensation, the molecular mechanism underlying ...
Cao, Xu, Cui, Yuanyuan, Wang, KeWei, Yang, Fan, Yarov-Yarovoy, Vladimir, Zheng, Jie   +5 more
core   +1 more source

On ionic conduction in potassium channels [PDF]

Proceedings of the National Academy of Sciences, 2010
In ref. 1, we presented an alternative mechanism for conduction of K+ in K+ channels where site vacancies are involved, and we proposed that coexistence of several ion permeation mechanisms is energetically possible. Specifically, we found that conduction can be described as a more anarchic phenomenon than previously habitually characterized by the ...
Carmen Domene, Carmen Domene, Simone Furini   +2 more
openaire   +4 more sources

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

The conduction pore of a cardiac potassium channel. [PDF]

, 1998
Ion channels form transmembrane water-filled pores that allow ions to cross membranes in a rapid and selective fashion. The amino acid residues that line these pores have been sought to reveal the mechanisms of ion conduction and selectivity. The pore (P)
Goldstein, SA, Tai, KK
core  

The response of the tandem pore potassium channel TASK-3 (K2P9.1) to voltage : gating at the cytoplasmic mouth [PDF]

, 2009
Although the tandem pore potassium channel TASK-3 is thought to open and shut at its selectivity filter in response to changes of extracellular pH, it is currently unknown whether the channel also shows gating at its inner, cytoplasmic mouth through ...
Andres-Enguix, Ashmole, Ashmole, Ben-Abu, Bichet, Boeckmann, Brickley, Carter, Chemin, Colquhoun, Colquhoun, Colquhoun, Cordero-Morales, Czirják, Czirják, D. V. Vavoulis, De La Cruz, Doyle, Duprat, Fanjul, Fersht, Gao, Goldstein, Han, Han, Hidalgo, Horovitz, I. Ashmole, J. F. Feng, Jiang, Jiang, Kim, Kim, Kim, Kuo, Leonoudakis, Lopes, Lopes, Lopes, M. J. Sutcliffe, Millar, Morton, P. J. Stansfeld, P. R. Stanfield, Patel, Puja R. Mehta, Rajan, Sigworth, Stanfield, Stansfeld, Talley, Talley, Veale, Washburn, Yellen, Yuill, Yuill, Zilberberg   +57 more
core   +2 more sources