Epigenetic quantification of immunosenescent CD8+ TEMRA cells in human blood
We studied old individuals over 65 years of age. We studied their CD4+ and CD8+ T cell subpopulations, quantified the CMV‐specific antibodies, inflammation‐associated proteins, and DNA methylation levels of selected CpGs. Based on our data we developed an epigenetic model to estimate CD8+ TEMRA cell levels in whole blood.
Ahto Salumets +11 more
wiley +1 more source
The association of cytomegalovirus and multiple sclerosis was assessed in a case–control study of presymptomatically collected samples. Cytomegalovirus seropositivity was found to reduce the risk of developing multiple sclerosis by 30%. Interactions between cytomegalovirus, Epstein–Barr virus and human herpesvirus 6A serostatus further impact the risk ...
Viktor Grut +20 more
wiley +1 more source
Tetraspanins CD9 and CD81 localize to the assembly compartment but not MViBs. [PDF]
S7A-B HFF cells were infected at an MOI of 1 with HCMV-pp150-SNAP-gM-mScarlet-I. Cells were fixed at 4 dpi and stained with specific antibodies for CD9 (⍺CD9) and CD81 (⍺CD81).
Timothy K. Soh (13199761) +9 more
core +1 more source
Tetraspanin CD63 localizes to MViBs. [PDF]
5A HFF cells were infected at an MOI of 1 with HCMV-pp150-SNAP-gM-mScarlet-I, fixed at 4 dpi, stained for CD63, and whole cells were imaged using confocal laser scanning microscopy. From a representative cell, two slices are shown.
Timothy K. Soh (13199761) +9 more
core +1 more source
SCP mediates pp150 binding to the capsid. [PDF]
(A–C) Density slices showing that pp150 tegument protein binds to the capsid triplex with its LHB (lower helix bundle). The binding sites on the triplex are labeled with “*”.
Sakar Shivakoti (446632) +10 more
core +1 more source
Human cytomegaloviruses expressing yellow fluorescent fusion proteins--characterization and use in antiviral screening. [PDF]
Recombinant viruses labelled with fluorescent proteins are useful tools in molecular virology with multiple applications (e.g., studies on intracellular trafficking, protein localization, or gene activity).
Sarah Straschewski +5 more
doaj +1 more source
Indonesian Cryptocurrencies Legislative Readiness: Lessons from the United States
Cryptocurrencies can facilitate cross-border global transfers easily and pseudonymously. It can be converted into fiat currencies, making it suitable for money laundering crimes.
Hanif Nur Widhiyanti +2 more
doaj +1 more source
Whole CMV proteome pattern recognition analysis after HSCT identifies unique epitope targets associated with the CMV status. [PDF]
Cytomegalovirus (CMV) infection represents a vital complication after Hematopoietic Stem Cell Transplantation (HSCT). We screened the entire CMV proteome to visualize the humoral target epitope-focus profile in serum after HSCT.
Lena Pérez-Bercoff +8 more
doaj +1 more source
EVAs are extracellular accumulations of viral products and other vesicular material. [PDF]
1A Overview indicates the subfigures’ positions in relation to the whole cell. 1B Quantification of EVA occurrence. HFF cells were infected with HCMV-pp150-SNAP-gM-mScarlet-I or HCMV-TB40-WT at an MOI of 1 and fixed at 120 hpi.
Timothy K. Soh (13199761) +9 more
core +1 more source
A CRISPR‐Cas9 tool to explore the genetics of Bacillus subtilis phages
Significance and Impact of the Study: This study presents a new CRISPR‐Cas9 vector specifically designed for the use with Bacillus phages. It is more efficient than existing systems, and in combination with the established vector‐specific construction of artificial CRISPR arrays, it will contribute to better exploitation and understanding of viral ...
K. Otte +6 more
wiley +1 more source

