Results 101 to 110 of about 9,210,402 (249)

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Analytical errors in the laboratory of a general hospital of the Thai Red Cross Society: A 3-year experience

Journal of Associated Medical Sciences
Background:Laboratory test results are critical to clinical decision-making, including patient screening, diagnosis, treatment planning, and monitoring therapeutic responses. Therefore, laboratory errors can significantly impact patient care and outcomes.
Srivilai Trakulkaseamsiri, Kittipong Chumchujan   +1 more
doaj   +1 more source

The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters [PDF]

Journal of Medical Biochemistry
Background: The pre-analytical (PA) phase is the most vulnerable phase of the laboratory testing procedure, with critical procedures-collection, handling, sample transport, and time and temperature of sample storage.
Gojković Amara, Vladimirov Sandra, Antonić Tamara, Bogavać-Stanojević Nataša, Novović Katarina, Spasojević-Kalimanovska Vesna, Filipić Brankica   +6 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Countercurrent chromatography in analytical chemistry (IUPAC technical report) [PDF]

, 2009
© 2009 IUPACCountercurrent chromatography (CCC) is a generic term covering all forms of liquid-liquid chromatography that use a support-free liquid stationary phase held in place by a simple centrifugal or complex centrifugal force field. Biphasic liquid
Sutherland, IA, Ian A. Sutherland, Tatyana Maryutina, Maryutina, T, Spivakov, B, Boris Spivakov, Shpigun, O, Berthod, A, Alain Berthod, Oleg Shpigun, Sutherland, Ian A., Iqbal, M. S., Torto, N., Lobinski, Ryszard, Labuda, J., Mester, Z., Motomizu, S., Trendafilova, N., Shpigun, Oleg, Smith, R. M., Knox, D. E., Lund, W., Fajgelj, A., Spivakov, Boris, Camoes, M., Maryutina, Tatyana, Berthod, Alain, Hibbert, B., Chai, Z., Apak, R., Minkkinen, P., Felinger, A., Aggarwal, S. K., Pingaron, J., Jarosz, M., Kim, H., de Zorzi, P., de Bieyre, P.   +37 more
core   +2 more sources

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Lipemic Samples: Underlying Mechanisms, Analytical Challenges, and Management

Korean Journal of Clinical Laboratory Science
Laboratory errors contribute significantly to overall healthcare errors, patient harm, and subsequent higher healthcare costs. Specifically, two-thirds of laboratory errors usually occur in the preanalytical phase.
Taha Sayed Mostafa MOHAMED
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source