Results 11 to 20 of about 106,307 (216)
Genome Biology, 2018 Understanding the functional impact of genomic variants is a major goal of modern genetics and personalized medicine. Although many synonymous and non-coding variants act through altering the efficiency of pre-mRNA splicing, it is difficult to predict ...
Scott I. Adamson +2 more
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The Drosophila splicing factor PSI is phosphorylated by casein kinase II and tousled-like kinase. [PDF]
PLoS ONE, 2013 Alternative splicing of pre-mRNA is a highly regulated process that allows cells to change their genetic informational output. These changes are mediated by protein factors that directly bind specific pre-mRNA sequences.
J Matthew Taliaferro +6 more
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BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. [PDF]
PLoS ONE, 2015 Previously, we showed that BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. In this study, we provide strong evidence that BCAS2 regulates the activity of Delta-Notch signaling via Delta pre-mRNA splicing.
Meng-Hsuan Chou +8 more
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Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development
eLife, 2014 Drosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being ...
Leonardo Gastón Guilgur +5 more
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The influence of 4-thiouridine labeling on pre-mRNA splicing outcomes.
PLoS ONE, 2021 Metabolic labeling is a widely used tool to investigate different aspects of pre-mRNA splicing and RNA turnover. The labeling technology takes advantage of native cellular machineries where a nucleotide analog is readily taken up and incorporated into ...
Jessie A C Altieri, Klemens J Hertel
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Six Exonic Variants in the SLC5A2 Gene Cause Exon Skipping in a Minigene Assay
Frontiers in Genetics, 2020 BackgroundFamilial renal glucosuria is a rare renal tubular disorder caused by SLC5A2 gene variants. Most of them are exonic variants and have been classified as missense variants.
Sai Wang +10 more
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A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants. [PDF]
PLoS Genetics, 2012 Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to allow
Laura-Oana Albulescu +6 more
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eLife, 2022 Splicing is highly regulated and is modulated by numerous factors. Quantitative predictions for how a mutation will affect precursor mRNA (pre-mRNA) structure and downstream function are particularly challenging.
Jayashree Kumar +7 more
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Cell Reports, 2019 Summary: In the eukaryotic cell, spliceosomes assemble onto pre-mRNA cotranscriptionally. Spliceosome assembly takes place in the context of the chromatin environment, suggesting that the state of the chromatin may affect splicing.
Calvin S. Leung +6 more
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G3: Genes, Genomes, Genetics, 2016 Pre-mRNA splicing is an essential component of eukaryotic gene expression and is highly conserved from unicellular yeasts to humans. Here, we present the development and implementation of a sequencing-based reverse genetic screen designed to identify ...
Amy Larson +2 more
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