Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8 [PDF]
, 2009 The U5 snRNA loop 1 aligns the 5' and 3' exons for ligation during the second step of pre-mRNA splicing. U5 is intimately associated with Prp8, which mediates pre-mRNA repositioning within the catalytic core of the spliceosome and interacts directly with
David Barrass, J. +5 more
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Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development
eLife, 2014 Drosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being ...
Leonardo Gastón Guilgur +5 more
doaj +1 more source
The influence of 4-thiouridine labeling on pre-mRNA splicing outcomes.
PLoS ONE, 2021 Metabolic labeling is a widely used tool to investigate different aspects of pre-mRNA splicing and RNA turnover. The labeling technology takes advantage of native cellular machineries where a nucleotide analog is readily taken up and incorporated into ...
Jessie A C Altieri, Klemens J Hertel
doaj +1 more source
Post-transcriptional regulation of 5-lipoxygenase mRNA expression via alternative splicing and nonsense-mediated mRNA decay [PDF]
, 2012 5-Lipoxygenase (5-LO) catalyzes the two initial steps in the biosynthesis of leukotrienes (LT), a group of inflammatory lipid mediators derived from arachidonic acid.
Laura Pufahl (182448) +26 more
core +2 more sources
Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]
, 2009 As evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Lewis, Joe +7 more
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A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants. [PDF]
PLoS Genetics, 2012 Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to allow
Laura-Oana Albulescu +6 more
doaj +1 more source
A mutation in a splicing factor that causes retinitis pigmentosa has a transcriptome-wide effect on mRNA splicing [PDF]
, 2014 Background: Substantial progress has been made in the identification of sequence elements that control mRNA splicing and the genetic variants in these elements that alter mRNA splicing (referred to as splicing quantitative trait loci – sQTLs).
Ramesar, Raj +7 more
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Six Exonic Variants in the SLC5A2 Gene Cause Exon Skipping in a Minigene Assay
Frontiers in Genetics, 2020 BackgroundFamilial renal glucosuria is a rare renal tubular disorder caused by SLC5A2 gene variants. Most of them are exonic variants and have been classified as missense variants.
Sai Wang +10 more
doaj +1 more source
Multiple tandem splicing silencer elements suppress aberrant splicing within the long exon 26 of the human Apolipoprotein B gene. [PDF]
, 2013 : BACKGROUND: Apolipoprotein B (APOB) is an integral component of the chylomicron and the atherogenic lipoproteins LDL and Lp(a). Exon 26 of the APOB pre-mRNA is unusually long at 7,572 nt and is constitutively spliced.
Srirangalingam, U +5 more
core +1 more source
eLife, 2022 Splicing is highly regulated and is modulated by numerous factors. Quantitative predictions for how a mutation will affect precursor mRNA (pre-mRNA) structure and downstream function are particularly challenging.
Jayashree Kumar +7 more
doaj +1 more source