The 35S U5 snRNP Is Generated from the Activated Spliceosome during In vitro Splicing. [PDF]
PLoS ONE, 2015 Primary gene transcripts of eukaryotes contain introns, which are removed during processing by splicing machinery. Biochemical studies In vitro have identified a specific pathway in which introns are recognised and spliced out. This occurs by progressive
Olga V Makarova, Evgeny M Makarov
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Surprisingly high number of Twintrons in vertebrates [PDF]
Biology Direct, 2013 Twintrons represent a special intronic arrangement in which introns of two different types occupy the same gene position. Consequently, alternative splicing of these introns requires two different spliceosomes competing for the same RNA molecule. So far,
Janice Jessin +2 more
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Structure of a step II catalytically activated spliceosome from Chlamydomonas reinhardtii [PDF]
The EMBO JournalPre-mRNA splicing, a fundamental step in eukaryotic gene expression, is executed by the spliceosomes. While there is extensive knowledge of the composition and structure of spliceosomes in yeasts and humans, the structural diversity of spliceosomes in ...
Yichen Lu, Ke Liang, Xiechao Zhan
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Crosstalk between chromatin state and ATM signalling in DNA damage-induced transcription stress [PDF]
The EMBO JournalThe DNA Damage Response (DDR) is a highly regulated process that safeguards genomic integrity against DNA lesions. Increasing evidence supports a reciprocal relationship between damaged chromatin architecture and the signalling pathways that coordinate ...
Irene Salas-Armenteros +3 more
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Nuclear Pre-snRNA Export Is an Essential Quality Assurance Mechanism for Functional Spliceosomes
Cell Reports, 2019 Summary: Removal of introns from pre-mRNAs is an essential step in eukaryotic gene expression, mediated by spliceosomes that contain snRNAs as key components. Although snRNAs are transcribed in the nucleus and function in the same compartment, all except
Thomas Lingner, Heike Krebber
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PRPF8-mediated dysregulation of hBrr2 helicase disrupts human spliceosome kinetics and 5´-splice-site selection causing tissue-specific defects [PDF]
Nature CommunicationsThe carboxy-terminus of the spliceosomal protein PRPF8, which regulates the RNA helicase Brr2, is a hotspot for mutations causing retinitis pigmentosa-type 13, with unclear role in human splicing and tissue-specificity mechanism.
Robert Atkinson +31 more
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Animals, 2022 In mammals, the testis-specific serine/threonine kinase (TSSK) is essential for spermatogenesis and male fertility. TSSK4 belongs to the family of the testis-specific serine/threonine-protein kinase (TSSK), with a crucial role in spermatogenesis.
Xingdong Wang +11 more
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Which Kind of Causal Specificity Matters Biologically? [PDF]
, 2017 Griffiths et al. (2015) have proposed a quantitative measure of causal specificity and used it to assess various attempts to single out genetic causes as being causally more specific than other cellular mechanisms, for example, alternative splicing ...
Weber, Marcel
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Frontiers in Genetics, 2021 Many eukaryotic species contain two separate molecular machineries for removing non-coding intron sequences from pre-mRNA molecules. The majority of introns (more than 99.5% in humans) are recognized and excised by the major spliceosome, which utilizes ...
Maureen V. Akinyi, Mikko J. Frilander
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Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription. [PDF]
, 2020 Deregulated expression of MYC induces a dependence on the NUAK1 kinase, but the molecular mechanisms underlying this dependence have not been fully clarified.
Ade, C.P. +18 more
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