Results 21 to 30 of about 8,766 (233)
Animals, 2020 In this study, the yak’s TLR4 gene alternative spliceosomes were investigated using PCR amplification and cloning to improve disease-resistance in yak and promote efficient utilization of yak’s resources.
Xingdong Wang +7 more
doaj +1 more source
Human spliceosomal snRNA sequence variants generate variant spliceosomes
RNA, 2021 Human pre-mRNA splicing is primarily catalyzed by the major spliceosome, comprising five small nuclear ribonucleoprotein complexes, U1, U2, U4, U5, and U6 snRNPs, each of which contains the corresponding U-rich snRNA. These snRNAs are encoded by large gene families exhibiting significant sequence variation, but it remains unknown if most human snRNA ...
Justin W. Mabin +3 more
openaire +2 more sources
eLife, 2016 The DEAH-box NTPase Prp43 and its cofactors Ntr1 and Ntr2 form the NTR complex and are required for disassembling intron-lariat spliceosomes (ILS) and defective earlier spliceosomes.
Jean-Baptiste Fourmann +7 more
doaj +1 more source
Structural basis for dual roles of Aar2p in U5 snRNP assembly [PDF]
, 2013 Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase.
Beggs, J.D. +8 more
core +1 more source
A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor [PDF]
, 1997 We have identified a human splicing factor required for the second step of pre-mRNA splicing. This new protein, hPrp18, is 30% identical to the yeast splicing factor Prp18.
Horowitz, D. S., Krainer, A. R.
core +1 more source
SnapShot: Spliceosome Dynamics I
Cell, 2015 Spliceosomes are multi-megadalton RNA-protein molecular machines that carry out pre-mRNA splicing, that is, the removal of non-coding intervening sequences (introns) from eukaryotic pre-mRNAs and the ligation of neighboring coding regions (exons) to produce mature mRNA for protein biosynthesis on the ribosome.
Wahl, M., Lührmann, R.
openaire +7 more sources
Bidirectional coupling of splicing and ATM signaling in response to transcription-blocking DNA damage [PDF]
, 2016 In response to DNA damage cells activate intricate protein networks to ensure genomic fidelity and tissue homeostasis. DNA damage response signaling pathways coordinate these networks and determine cellular fates, in part, by modulating RNA metabolism ...
Marteijn, J.A. (Jurgen) +2 more
core +3 more sources
Cloning and tissue expression of alternative spliceosome in chicken G-protein alpha subunit gene
浙江大学学报. 农业与生命科学版, 2019 In order to research deeply the transcription and splicing situations of chicken G-protein alpha subunit gene (GNAS), we used 5´ and 3´ rapid-amplification cDNA end (RACE) technology to clone and sequence the chicken GNAS gene, and used quantitative ...
ZHANG Xuedong +6 more
doaj +1 more source
Therapeutic Targeting of Alternative Splicing: A New Frontier in Cancer Treatment
Frontiers in Oncology, 2022 The ability for cells to harness alternative splicing enables them to diversify their proteome in order to carry out complex biological functions and adapt to external and internal stimuli. The spliceosome is the multiprotein-RNA complex charged with the
Anthony J. Murphy +3 more
doaj +1 more source
Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
doaj +1 more source