Results 11 to 20 of about 6,735 (249)
Animals, 2020 In this study, the yak’s TLR4 gene alternative spliceosomes were investigated using PCR amplification and cloning to improve disease-resistance in yak and promote efficient utilization of yak’s resources.
Xingdong Wang +7 more
doaj +2 more sources
Stem Cell Research, 2017 Spliceosomes are the core host of pre-mRNA splicing, allowing multiple protein isoforms to be produced from a single gene. Herein, we reveal that spliceosomes are more abundant in human pluripotent stem cells (hPSs), including human embryonic stem cells (
Young-Dae Kim +8 more
doaj +2 more sources
Spliceosomal snRNA Epitranscriptomics [PDF]
Frontiers in Genetics, 2021 Small nuclear RNAs (snRNAs) are critical components of the spliceosome that catalyze the splicing of pre-mRNA. snRNAs are each complexed with many proteins to form RNA-protein complexes, termed as small nuclear ribonucleoproteins (snRNPs), in the cell ...
Pedro Morais, Hironori Adachi, Yi-Tao Yu
doaj +3 more sources
Synergistic assembly of human pre-spliceosomes across introns and exons
eLife, 2018 Most human genes contain multiple introns, necessitating mechanisms to effectively define exons and ensure their proper connection by spliceosomes. Human spliceosome assembly involves both cross-intron and cross-exon interactions, but how these work ...
Joerg E Braun +3 more
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Multiple quality control checkpoints safeguard small nuclear RNA biogenesis and prevent assembly of aberrant spliceosomes. [PDF]
Genes DevDefective small nuclear (sn)RNAs are produced from hundreds of human snRNA pseudogenes and mutant snRNA genes associated with human developmental disorders. Machineries that prevent defective snRNAs from disrupting pre-mRNA splicing remain poorly defined.
Ma T +5 more
europepmc +2 more sources
Regulation of Hair Follicle Growth and Development by Different Alternative Spliceosomes of FGF5 in Rabbits. [PDF]
Genes (Basel)This study investigated the regulatory effect of alternative spliceosomes of the fibroblast growth factor 5 (FGF5) gene on hair follicle (HF) growth and development in rabbits.
Sun S +9 more
europepmc +2 more sources
Spliceosome Database: a tool for tracking components of the spliceosome [PDF]
Nucleic Acids Research, 2012 The spliceosome is the extremely complex macromolecular machine responsible for pre-mRNA splicing. It assembles from five U-rich small nuclear RNAs (snRNAs) and over 200 proteins in a highly dynamic fashion. One important challenge to studying the spliceosome is simply keeping track of all these proteins, a situation further complicated by the variety ...
Cvitkovic, Ivan, Jurica, Melissa S
openaire +4 more sources
Spliceosomal immunophilins [PDF]
FEBS Letters, 2008 The spliceosome is a dynamic, macromolecular complex, which removes non‐protein‐coding introns from pre‐mRNA to form mature mRNA in a process known as splicing. This ribonucleoprotein assembly is comprised of five uridine‐rich small nuclear RNAs (snRNAs) as well as over 300 proteins.
Mesa, Annia +2 more
openaire +2 more sources
Spliceosomal intronogenesis [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Eukaryotic transcripts contain spliceosomal introns that need to be removed by pre-mRNA splicing. Although several models have been proposed to identify the mechanism of intron gain over the evolution of eukaryotes, they remain models due to a lack of experimental validation.
Sujin, Lee, Scott W, Stevens
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Purification and electron microscopic visualization of functional human spliceosomes [PDF]
, 2002 Pre-mRNA splicing takes place in a large and highly dynamic complex known as the spliceosome. Here we report the optimization of a maltose-binding protein (MBP) affinity-purification method to isolate functional spliceosomes for electron microscopic ...
Griffith, J. +7 more
core +1 more source